FastQCFastQC Report
Thu 26 May 2016
SRR1546847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546847_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2516114
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518992.062664887203044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT274100.043.9410821
CGCATCG1650.040.9090921
CACGACG3600.039.37526
CGGTCTA3550.039.29577631
GCGCGAC7450.038.9597329
GTTTTTT319150.038.7748682
CGACGGT3850.038.57142628
GGGCGAT34700.037.2190177
TACGGGA6900.036.8478284
ACGGGAT6050.036.8181845
GCGTAAG1550.036.290321
AGGGCGA17750.036.253526
CGCGACC7950.036.22641410
GTAGGGT11350.036.0792964
TAACGCC2500.036.00000412
CCGTAGA250.002107584435.99999617
CGTAGTC250.002107584435.99999632
GCGTATC250.002107584435.99999645
TATGGGC9400.035.9042554
TCACGAC3950.035.88607825
Produced by FastQC (version 0.11.5)