##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546847_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2516114
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938615261470666	33.0	31.0	34.0	30.0	34.0
2	32.3184557615434	34.0	31.0	34.0	31.0	34.0
3	32.28582846405211	34.0	31.0	34.0	30.0	34.0
4	35.95680442142129	37.0	35.0	37.0	35.0	37.0
5	35.9365982622409	37.0	35.0	37.0	35.0	37.0
6	35.99053143061086	37.0	35.0	37.0	35.0	37.0
7	36.179554265029324	37.0	35.0	37.0	35.0	37.0
8	36.0749878582608	37.0	35.0	37.0	35.0	37.0
9	37.888994695788824	39.0	38.0	39.0	35.0	39.0
10	37.63421728904175	39.0	37.0	39.0	35.0	39.0
11	37.46893304516409	39.0	37.0	39.0	35.0	39.0
12	37.0076081608385	39.0	35.0	39.0	33.0	39.0
13	36.91023618166744	39.0	35.0	39.0	33.0	39.0
14	38.133401745707864	40.0	36.0	41.0	33.0	41.0
15	38.22365401567656	40.0	36.0	41.0	33.0	41.0
16	38.28767853920768	40.0	36.0	41.0	34.0	41.0
17	38.20793533202391	40.0	36.0	41.0	34.0	41.0
18	37.97727130010802	39.0	36.0	41.0	34.0	41.0
19	37.810788382402386	39.0	36.0	41.0	34.0	41.0
20	37.55911934037965	39.0	35.0	41.0	34.0	41.0
21	37.43973563995908	39.0	35.0	41.0	33.0	41.0
22	37.38950222446201	39.0	35.0	41.0	33.0	41.0
23	37.32658059213533	39.0	35.0	41.0	33.0	41.0
24	37.304581588910516	39.0	35.0	41.0	33.0	41.0
25	37.180419090708924	39.0	35.0	41.0	33.0	41.0
26	37.15856356270026	39.0	35.0	41.0	33.0	41.0
27	37.09111073663594	39.0	35.0	41.0	33.0	41.0
28	36.9506727437628	39.0	35.0	41.0	33.0	41.0
29	36.74214284408417	38.0	35.0	41.0	32.0	41.0
30	36.65859019106448	38.0	35.0	41.0	32.0	41.0
31	36.39182882810556	38.0	35.0	41.0	31.0	41.0
32	36.076927754465814	38.0	35.0	41.0	30.0	41.0
33	35.710080703815485	38.0	35.0	41.0	28.0	41.0
34	35.32605239667201	38.0	35.0	41.0	24.0	41.0
35	35.077537822213145	38.0	35.0	41.0	23.0	41.0
36	34.94068233792269	38.0	35.0	41.0	23.0	41.0
37	34.692821946859326	38.0	34.0	41.0	21.0	41.0
38	34.74571780133969	38.0	35.0	41.0	22.0	41.0
39	34.64358848605429	38.0	34.0	41.0	21.0	41.0
40	34.53651543610504	38.0	34.0	40.0	21.0	41.0
41	34.422567498928906	38.0	34.0	40.0	20.0	41.0
42	34.39162613458691	37.0	34.0	40.0	20.0	41.0
43	34.24914888594078	37.0	34.0	40.0	19.0	41.0
44	34.095893111361406	37.0	34.0	40.0	18.0	41.0
45	34.05946153473173	37.0	34.0	40.0	20.0	41.0
46	33.88535137915055	36.0	34.0	40.0	18.0	41.0
47	33.89254064005049	36.0	34.0	40.0	20.0	41.0
48	33.73133292052745	36.0	33.0	40.0	18.0	41.0
49	33.657477363903226	36.0	33.0	40.0	18.0	41.0
50	33.525914565079326	36.0	33.0	40.0	18.0	41.0
51	33.02376998816429	35.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	4.0
12	8.0
13	15.0
14	29.0
15	73.0
16	171.0
17	424.0
18	900.0
19	1905.0
20	3361.0
21	5662.0
22	8938.0
23	13631.0
24	21649.0
25	36612.0
26	55551.0
27	63650.0
28	59557.0
29	53905.0
30	53032.0
31	57596.0
32	68325.0
33	88400.0
34	157798.0
35	289026.0
36	172911.0
37	211506.0
38	337763.0
39	753392.0
40	314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.930599328965222	22.083260138451596	26.52065844393378	21.465482088649402
2	38.638869304014044	22.911124058766813	25.96344998676531	12.486556650453835
3	26.78841260769584	23.14696392929732	37.18364907154446	12.880974391462392
4	24.400325263481704	24.798558411900256	36.27319747833365	14.52791884628439
5	21.620443270853386	28.434522442146896	35.522118632144654	14.422915654855068
6	19.28497675383548	37.124470512862295	33.35087360906541	10.239679124236819
7	78.48646762428093	4.2024327991498005	14.996975494751034	2.3141240818182323
8	78.8951136554226	3.67670145311381	14.834343753899862	2.5938411375637194
9	74.09501318302748	5.5133829389288405	16.691652286025196	3.6999515920184853
10	39.45135236320771	24.54364150431976	23.279032667041317	12.725973465431217
11	32.9318544390278	22.662526419709124	29.100628985809067	15.304990155454007
12	28.619768420667747	20.17587438407004	34.99583087252803	16.208526322734183
13	22.806001635855928	25.976366730601235	34.48619577650297	16.731435857039862
14	17.53926888845259	30.023122958657677	35.57028815069587	16.867320002193857
15	17.105385527046867	22.751393617300327	43.747024180939334	16.39619667471347
16	19.750973127608685	20.911612112964676	41.57144708069666	17.76596767872998
17	19.863925084475504	20.781053640653802	34.1032640015516	25.251757273319093
18	22.992996342773022	22.970421848930535	35.50884419386403	18.527737614432414
19	25.563229646987377	24.70619375751655	31.446587873204475	18.283988722291596
20	25.35028222091686	23.555371497475868	31.14445529892525	19.949890982682025
21	23.973436815660975	24.984082597211412	33.02247036501525	18.020010222112354
22	21.953735005647598	23.139531833613262	33.36255829425853	21.54417486648061
23	20.336479189734646	25.616883813690478	32.65436303760482	21.392273958970062
24	20.86972211910907	23.784931843310755	35.924644113899454	19.420701923680724
25	20.692067211581033	24.4828731925501	34.176034949131875	20.64902464673699
26	18.300561898228775	26.81957176821082	32.79100231547537	22.08886401808503
27	19.382388874271992	25.270516359751582	34.393990097428016	20.953104668548406
28	18.948426025211894	24.84676767427867	37.18563626290383	19.01917003760561
29	19.043970185770597	25.329973125224054	36.309086154283946	19.3169705347214
30	21.383530316988818	24.8314265569843	34.85732363477966	18.927719491247217
31	24.672053809962506	24.999662177468906	30.888425564183496	19.43985844838509
32	25.20183902637162	26.639969413150595	30.29175148661786	17.86644007385993
33	24.30144262143925	25.99158861641404	30.535579866413048	19.17138889573366
34	21.637692091852752	27.070871987517258	31.64117365111438	19.65026226951561
35	21.57549300230435	27.876280645471546	30.6773063541636	19.8709199980605
36	25.116707748536037	26.744893116925546	29.215170695763387	18.92322843877503
37	22.462138043029846	29.036482448728474	28.926709998036653	19.574669510205023
38	21.93747978032792	29.37903449525737	27.466720506304565	21.216765218110147
39	22.68414706169911	27.544777382900776	28.31823200379633	21.45284355160378
40	23.059169815040175	25.82883764408131	29.0846519672797	22.02734057359881
41	20.22428236558439	25.29221648939595	29.84626292767339	24.637238217346273
42	22.124156536627517	27.711224531161943	27.76225560527067	22.402363326939874
43	21.698897585721475	26.939836589280137	28.68486880960084	22.676397015397555
44	21.070786140850533	27.942215654775577	29.655731020136606	21.33126718423728
45	20.585434523237023	28.99463219870006	28.081279306104573	22.338653971958344
46	22.480499691190463	28.456103340309703	29.044510701820347	20.01888626667949
47	21.913395021052306	26.863528441080177	29.74169691834313	21.481379619524393
48	22.018318724827253	25.79728104529445	30.27156162240662	21.91283860747168
49	21.766501835767375	25.12640524236978	31.15876307671274	21.9483298451501
50	20.23040291497126	26.93526604915358	31.184238869939918	21.65009216593525
51	20.433692591035225	28.185606852471707	29.367786992163314	22.012913564329757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2079.0
1	3255.5
2	4432.0
3	43099.5
4	81767.0
5	54566.0
6	27365.0
7	27182.5
8	27000.0
9	28140.5
10	29281.0
11	29386.0
12	29491.0
13	28524.0
14	27557.0
15	25840.5
16	24124.0
17	22495.0
18	20866.0
19	19403.5
20	17941.0
21	17539.0
22	17137.0
23	17199.0
24	17261.0
25	18369.5
26	20267.5
27	21057.0
28	24619.0
29	28181.0
30	32834.5
31	37488.0
32	41176.0
33	44864.0
34	50845.5
35	56827.0
36	63491.0
37	70155.0
38	76677.5
39	83200.0
40	99921.0
41	116642.0
42	132283.0
43	147924.0
44	157670.0
45	167416.0
46	176734.0
47	186052.0
48	188562.0
49	191072.0
50	182081.0
51	173090.0
52	160592.5
53	148095.0
54	139027.5
55	129960.0
56	124523.5
57	119087.0
58	114410.5
59	109734.0
60	103106.0
61	96478.0
62	86698.5
63	76919.0
64	68505.0
65	60091.0
66	49659.5
67	39228.0
68	31618.5
69	24009.0
70	21122.5
71	18236.0
72	15277.0
73	12318.0
74	9232.0
75	4716.0
76	3286.0
77	2364.5
78	1443.0
79	1075.0
80	707.0
81	569.0
82	431.0
83	266.0
84	101.0
85	71.0
86	41.0
87	33.0
88	25.0
89	18.0
90	11.0
91	9.0
92	7.0
93	6.0
94	5.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2516114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.11820822322615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6790608908957	20.81074303379529
2	6.491532423336823	3.3909439104106993
3	2.5405760026648285	1.9906587913359464
4	1.4657306623465542	1.5312903455333795
5	0.9777652120700576	1.2768737701136317
6	0.718990423621459	1.1267244956790505
7	0.5538808433755483	1.0126462638697076
8	0.4826569818813146	1.0084908442536054
9	0.39160042709773984	0.9205111345718754
>10	5.111669253684218	32.181202225184876
>50	1.4115648132358198	25.173203832176167
>100	0.16780537106171012	5.7628531983928974
>500	0.004985526767443316	0.9263842983751541
>1k	0.002025370249273847	0.775622028961118
>5k	0.0	0.0
>10k+	1.5579771148260363E-4	2.111851827346575
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51899	2.062664887203044	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5897530874992152E-4	0.0	0.0	0.02209756791623909	0.0
2	1.5897530874992152E-4	0.0	0.0	0.09443133339745337	0.0
3	1.5897530874992152E-4	0.0	0.0	0.18484854024897124	0.0
4	1.5897530874992152E-4	0.0	0.0	0.2879440279732953	0.0
5	1.5897530874992152E-4	0.0	0.0	0.5355878151784855	0.0
6	1.5897530874992152E-4	0.0	0.0	1.0365985007038632	0.0
7	1.5897530874992152E-4	0.0	0.0	1.403831463916182	0.0
8	1.5897530874992152E-4	0.0	0.0	2.0919958316674045	0.0
9	1.5897530874992152E-4	0.0	0.0	2.5583498998852994	0.0
10	1.5897530874992152E-4	0.0	0.0	3.1446110947278223	0.0
11	1.5897530874992152E-4	0.0	0.0	3.4770682091510956	0.0
12	1.5897530874992152E-4	0.0	0.0	3.799827829740624	0.0
13	1.5897530874992152E-4	0.0	0.0	4.014762447170518	0.0
14	1.5897530874992152E-4	0.0	0.0	4.125409262060463	0.0
15	1.5897530874992152E-4	0.0	0.0	4.201478947297301	0.0
16	2.782067903123626E-4	0.0	0.0	4.308946256012248	0.0
17	2.782067903123626E-4	0.0	0.0	4.419911021519693	0.0
18	2.782067903123626E-4	0.0	0.0	4.627334055611152	0.0
19	2.782067903123626E-4	0.0	0.0	4.7089678766542375	0.0
20	2.782067903123626E-4	0.0	0.0	4.813414654502936	0.0
21	2.782067903123626E-4	0.0	0.0	4.919928111365383	0.0
22	2.782067903123626E-4	0.0	0.0	5.032204423170016	0.0
23	2.782067903123626E-4	0.0	0.0	5.158589793626203	0.0
24	2.782067903123626E-4	0.0	0.0	5.258982701101778	0.0
25	2.782067903123626E-4	0.0	0.0	5.341888324614862	0.0
26	2.782067903123626E-4	0.0	0.0	5.431113216650756	0.0
27	2.782067903123626E-4	0.0	0.0	5.531466380299144	0.0
28	2.782067903123626E-4	0.0	0.0	5.625261812461598	0.0
29	2.782067903123626E-4	0.0	0.0	5.7331265594484195	0.0
30	2.782067903123626E-4	0.001589753087499215	0.0	5.877714602756473	0.0
31	2.782067903123626E-4	0.001589753087499215	0.0	5.9963101830839145	0.0
32	2.782067903123626E-4	0.001589753087499215	0.0	6.122576322058539	0.0
33	3.1795061749984304E-4	0.001589753087499215	0.0	6.282108044389085	0.0
34	3.9743827187480377E-4	0.001589753087499215	0.0	6.448674424131816	0.0
35	3.9743827187480377E-4	0.001589753087499215	0.0	6.601409952013303	0.0
36	3.9743827187480377E-4	0.001589753087499215	0.0	6.726920958271366	0.0
37	3.9743827187480377E-4	0.001589753087499215	0.0	6.860539705275675	0.0
38	3.9743827187480377E-4	0.001589753087499215	0.0	7.019435526371222	0.0
39	3.9743827187480377E-4	0.001589753087499215	0.0	7.24613431664861	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27410	0.0	43.941082	1
CGCATCG	165	0.0	40.90909	21
CACGACG	360	0.0	39.375	26
CGGTCTA	355	0.0	39.295776	31
GCGCGAC	745	0.0	38.959732	9
GTTTTTT	31915	0.0	38.774868	2
CGACGGT	385	0.0	38.571426	28
GGGCGAT	3470	0.0	37.219017	7
TACGGGA	690	0.0	36.847828	4
ACGGGAT	605	0.0	36.818184	5
GCGTAAG	155	0.0	36.29032	1
AGGGCGA	1775	0.0	36.25352	6
CGCGACC	795	0.0	36.226414	10
GTAGGGT	1135	0.0	36.079296	4
TAACGCC	250	0.0	36.000004	12
CCGTAGA	25	0.0021075844	35.999996	17
CGTAGTC	25	0.0021075844	35.999996	32
GCGTATC	25	0.0021075844	35.999996	45
TATGGGC	940	0.0	35.904255	4
TCACGAC	395	0.0	35.886078	25
>>END_MODULE