FastQCFastQC Report
Thu 26 May 2016
SRR1546846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546846_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1970995
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82940.42080269102661344No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG71120.36083298029675365No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC40620.20608880286352832No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC35620.1807209049236553No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25330.12851377096339667No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC207.0345146E-445.00000436
CGGTCTA3600.041.87531
CGTTTTT61850.041.8714641
TATAGCG1300.041.5384641
CGCATCG1100.040.90909221
CGTATGG1600.040.7812542
CGATGAA19800.039.88636419
TCGATTG800.039.3750041
ACGTTAG750.039.0000041
AGTACGG3700.038.918922
CACGACG4100.038.41463526
GATGAAT21150.038.4042520
CCGATGA20050.038.1546118
TCACGAC4150.037.95180525
GGGCGAT27900.037.5806437
ACGTTAC301.14021794E-437.50000424
CGTAGAT301.14021794E-437.50000442
GCGATCG301.14021794E-437.50000433
AGGGCGA13050.037.4137956
TCTCACG4200.036.96428723
Produced by FastQC (version 0.11.5)