##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546846_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1970995
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9585432738287	33.0	31.0	34.0	30.0	34.0
2	32.350453958533635	34.0	31.0	34.0	31.0	34.0
3	32.41841607918843	34.0	31.0	34.0	30.0	34.0
4	36.01535265183321	37.0	35.0	37.0	35.0	37.0
5	35.96896745045015	37.0	35.0	37.0	35.0	37.0
6	36.02894680098123	37.0	35.0	37.0	35.0	37.0
7	36.210313065228476	37.0	35.0	37.0	35.0	37.0
8	36.06366936496541	37.0	35.0	37.0	35.0	37.0
9	37.89262022481031	39.0	39.0	39.0	35.0	39.0
10	37.655666807881296	39.0	37.0	39.0	35.0	39.0
11	37.44094327991699	39.0	37.0	39.0	35.0	39.0
12	36.84669925595956	39.0	35.0	39.0	33.0	39.0
13	36.65411936610697	39.0	35.0	39.0	33.0	39.0
14	37.81944449377091	40.0	35.0	41.0	33.0	41.0
15	37.929524428017324	40.0	35.0	41.0	33.0	41.0
16	38.036462801782854	40.0	35.0	41.0	34.0	41.0
17	37.93012209569279	40.0	35.0	41.0	34.0	41.0
18	37.883569973541285	40.0	36.0	41.0	34.0	41.0
19	37.85496665389816	40.0	36.0	41.0	34.0	41.0
20	37.65694230578971	40.0	35.0	41.0	34.0	41.0
21	37.59098983000972	40.0	35.0	41.0	33.0	41.0
22	37.531033310586785	39.0	35.0	41.0	33.0	41.0
23	37.427813363301276	39.0	35.0	41.0	33.0	41.0
24	37.45901181890365	39.0	35.0	41.0	33.0	41.0
25	37.40646323303712	39.0	35.0	41.0	33.0	41.0
26	37.335598517500046	39.0	35.0	41.0	33.0	41.0
27	37.247266989515445	39.0	35.0	41.0	33.0	41.0
28	37.20797110089067	39.0	35.0	41.0	33.0	41.0
29	37.12152643715484	39.0	35.0	41.0	33.0	41.0
30	37.10049594240473	39.0	35.0	41.0	33.0	41.0
31	36.928303724768455	39.0	35.0	41.0	32.0	41.0
32	36.77140429072626	39.0	35.0	41.0	32.0	41.0
33	36.65506305191033	39.0	35.0	41.0	31.0	41.0
34	36.47293372129305	39.0	35.0	41.0	31.0	41.0
35	36.35112316368129	39.0	35.0	41.0	31.0	41.0
36	36.288092055028045	39.0	35.0	41.0	31.0	41.0
37	36.02392953812668	39.0	35.0	41.0	30.0	41.0
38	36.112900337139365	39.0	35.0	41.0	30.0	41.0
39	36.026270487748576	38.0	35.0	41.0	30.0	41.0
40	35.939753779182595	38.0	35.0	41.0	30.0	41.0
41	35.8865324366627	38.0	35.0	41.0	30.0	41.0
42	35.89423007161358	38.0	35.0	41.0	30.0	41.0
43	35.797803140038404	38.0	35.0	41.0	30.0	41.0
44	35.71045487177796	38.0	35.0	40.0	29.0	41.0
45	35.66613055842354	38.0	35.0	40.0	29.0	41.0
46	35.503199145609194	38.0	35.0	40.0	29.0	41.0
47	35.43824565765007	37.0	35.0	40.0	29.0	41.0
48	35.3644316702985	37.0	35.0	40.0	29.0	41.0
49	35.344522943995294	37.0	35.0	40.0	28.0	41.0
50	35.211079175746264	37.0	35.0	40.0	28.0	41.0
51	34.68672117382337	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	9.0
13	12.0
14	18.0
15	37.0
16	94.0
17	206.0
18	536.0
19	1152.0
20	2109.0
21	3835.0
22	6489.0
23	9905.0
24	13052.0
25	17420.0
26	22959.0
27	25989.0
28	26573.0
29	28186.0
30	31798.0
31	38721.0
32	49656.0
33	67509.0
34	133441.0
35	280632.0
36	116842.0
37	159010.0
38	270412.0
39	664107.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.01196350066844	22.18128407225792	28.139290054008253	13.667462373065382
2	31.78343932886689	26.14075631850918	28.96298570011593	13.112818652507997
3	29.31904951559999	26.571300282344705	30.582624511985063	13.527025690070243
4	26.05425178653421	26.065819547994796	32.36274064622183	15.517188019249161
5	25.102701934809573	30.76258437997052	28.62944857800248	15.50526510721742
6	20.975344939992237	41.30715704504578	26.81194016220234	10.905557852759646
7	84.7058465394382	4.448869733307289	8.820874735856762	2.024408991397746
8	85.43040444039686	5.360591985266325	6.782158250020928	2.426845324315891
9	80.9786935025203	4.953234280147844	8.553700034754021	5.5143721825778345
10	46.15815869649593	25.92944172867004	15.006126347352481	12.906273227481552
11	39.55479339115523	22.618017803190774	21.934099274731796	15.8930895309222
12	34.7302758251543	22.04729083533951	25.96921859263976	17.25321474686643
13	23.584382507312295	32.386180583918275	27.288501492900796	16.740935415868634
14	17.517598979195785	36.701158551898914	28.27399359206898	17.507248876836318
15	16.24291284351305	24.470330974964423	41.823444503918076	17.46331167760446
16	19.129728893274716	21.22095692784609	38.820595688979424	20.82871848989977
17	19.842871240160427	22.02354648286779	28.86719651749497	29.266385759476815
18	23.251555686341163	24.176266302045413	30.867607477441595	21.704570534171825
19	26.567647305041366	25.528375262240644	25.94699631404443	21.956981118673564
20	29.34259092488819	24.533547776630584	25.748568616358742	20.37529268212248
21	24.583877686143293	27.86658515115462	27.98601721465554	19.563519948046544
22	23.5277613591105	23.273169135385935	29.023056882437547	24.176012623066015
23	21.993307948523462	28.194084713558382	27.05288445683525	22.759722881082904
24	21.43638111715149	26.514729869938787	32.59531353453459	19.453575478375136
25	20.247032590138485	27.126299153473244	31.15299632926517	21.473671927123103
26	18.784573273904805	30.87882008833102	27.933556401715887	22.40305023604829
27	20.49203574844178	29.334118046976272	29.276228503877483	20.897617700704465
28	17.661384224718987	28.003724007417574	35.30085058561793	19.034041182245513
29	19.361895895220435	26.407525133244885	33.78141497061129	20.449164000923393
30	20.522578697561382	28.506312801402338	30.73488263542018	20.2362258656161
31	26.972823370937014	25.953033873754116	25.22735978528611	21.846782970022755
32	28.163643236030534	28.07774753360612	25.5329922196657	18.225617010697643
33	26.013358735055135	26.4528321989655	26.073379181580876	21.460429884398486
34	21.006141568091245	27.28941473722663	29.052788058823083	22.651655635859047
35	22.068295454833724	26.098391928949592	29.21509186984239	22.61822074637429
36	26.726856232512002	26.719753221088844	26.603365305340702	19.95002524105845
37	22.268397433783445	28.87708999769152	28.206007625590118	20.648504942934913
38	22.244653081311725	30.763396152704598	23.93775732561473	23.05419344036895
39	21.896656257372545	28.775263255360873	26.52548585866529	22.80259462860129
40	22.56246210670245	25.74643771293179	26.716404658560776	24.974695521804975
41	19.483763276923586	24.714623832125397	27.99215624595699	27.809456644994025
42	22.45885961151601	27.336599027394794	25.364549377344947	24.83999198374425
43	22.20365855824089	27.39322017559659	25.64785806153745	24.755263204625074
44	21.938208874198057	28.974046103617717	27.917879040789046	21.169865981395183
45	20.40167529597995	30.844421218724555	25.772211497238708	22.98169198805679
46	23.495290449747465	29.56983655463357	26.76637941750233	20.168493578116635
47	22.247595757472748	27.483682099650174	28.181299292996687	22.08742284988039
48	23.127202250639904	25.69534676648089	28.94467007780334	22.232780905075863
49	21.201981740187062	24.827206563182553	30.504846536901415	23.46596515972897
50	20.65550648276632	28.346545780177017	27.89814281619182	23.09980492086484
51	20.34343060231	30.4562923802445	26.507018028965067	22.693258988480437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1018.0
1	2030.5
2	3043.0
3	9474.0
4	15905.0
5	11451.0
6	6997.0
7	7142.5
8	7288.0
9	7667.0
10	8046.0
11	7988.0
12	7930.0
13	7821.5
14	7713.0
15	7340.5
16	6968.0
17	6569.5
18	6171.0
19	6100.0
20	6029.0
21	6415.0
22	6801.0
23	7672.5
24	8544.0
25	9549.5
26	12486.5
27	14418.0
28	17352.5
29	20287.0
30	24575.5
31	28864.0
32	33053.5
33	37243.0
34	42562.5
35	47882.0
36	54397.5
37	60913.0
38	70489.0
39	80065.0
40	100273.0
41	120481.0
42	140623.0
43	160765.0
44	162077.0
45	163389.0
46	172497.0
47	181605.0
48	173647.0
49	165689.0
50	160349.5
51	155010.0
52	145356.5
53	135703.0
54	124004.0
55	112305.0
56	102365.0
57	92425.0
58	85429.0
59	78433.0
60	71631.0
61	64829.0
62	57786.5
63	50744.0
64	42901.0
65	35058.0
66	29570.5
67	24083.0
68	19383.5
69	14684.0
70	11996.0
71	9308.0
72	7732.5
73	6157.0
74	5095.5
75	2997.0
76	1960.0
77	1433.5
78	907.0
79	611.5
80	316.0
81	278.0
82	240.0
83	180.0
84	120.0
85	76.0
86	32.0
87	20.0
88	8.0
89	8.5
90	9.0
91	7.0
92	5.0
93	8.0
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1970995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.097920285316206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10242699092622	16.29990112609313
2	6.287433774517803	2.5272868559892725
3	1.9918089789092361	1.2009365424508471
4	1.0804670090057131	0.868605592716434
5	0.6988591341275947	0.7022807584180754
6	0.5246850592421684	0.6327047097327307
7	0.4091331954508431	0.5755908443773418
8	0.3222265288174168	0.518086647198927
9	0.2852595103299633	0.5159810609315942
>10	4.313825821828419	22.100871700850703
>50	2.2234071406280105	32.0415783005795
>100	0.7520314255570061	19.50161793142562
>500	0.005112382218000839	0.7192377519981425
>1k	0.0028118102199004616	1.003850405288836
>5k	5.11238221800084E-4	0.7914697719488132
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8294	0.42080269102661344	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	7112	0.36083298029675365	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	4062	0.20608880286352832	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	3562	0.1807209049236553	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2533	0.12851377096339667	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.073579587974602E-5	0.0	0.0	0.0286657246720565	0.0
2	5.073579587974602E-5	0.0	0.0	0.1320652766749789	0.0
3	5.073579587974602E-5	0.0	0.0	0.1853378623487122	0.0
4	5.073579587974602E-5	0.0	0.0	0.3095898264582102	0.0
5	5.073579587974602E-5	0.0	0.0	0.6776780255657675	0.0
6	5.073579587974602E-5	0.0	0.0	0.9051265984946689	0.0
7	5.073579587974602E-5	0.0	0.0	1.0967556995324697	0.0
8	5.073579587974602E-5	0.0	0.0	1.4708814583497167	0.0
9	5.073579587974602E-5	0.0	0.0	1.6356713233671318	0.0
10	5.073579587974602E-5	0.0	0.0	2.285038774832001	0.0
11	5.073579587974602E-5	0.0	0.0	2.5382611320678135	0.0
12	5.073579587974602E-5	0.0	0.0	2.957998371380952	0.0
13	5.073579587974602E-5	0.0	0.0	3.068145784235881	0.0
14	5.073579587974602E-5	0.0	0.0	3.1137572647317726	0.0
15	5.073579587974602E-5	0.0	0.0	3.205335376294714	0.0
16	5.073579587974602E-5	0.0	0.0	3.3391256700296044	0.0
17	5.073579587974602E-5	0.0	0.0	3.469364458052912	0.0
18	5.073579587974602E-5	0.0	0.0	3.6736775080606496	0.0
19	5.073579587974602E-5	0.0	0.0	3.8215723530501093	0.0
20	5.073579587974602E-5	0.0	0.0	3.924667490277753	0.0
21	5.073579587974602E-5	0.0	0.0	4.055464372055738	0.0
22	5.073579587974602E-5	0.0	0.0	4.1877833277101155	0.0
23	5.073579587974602E-5	0.0	0.0	4.328422953888772	0.0
24	5.073579587974602E-5	0.0	0.0	4.440549062783011	0.0
25	5.073579587974602E-5	0.0	0.0	4.527104330553858	0.0
26	5.073579587974602E-5	0.0	0.0	4.620255251789071	0.0
27	5.073579587974602E-5	0.0	0.0	4.725836443014822	0.0
28	5.073579587974602E-5	0.0	0.0	4.823147699512175	0.0
29	5.073579587974602E-5	0.0	0.0	4.929185512900845	0.0
30	5.073579587974602E-5	0.0	0.0	5.074797247075716	0.0
31	5.073579587974602E-5	0.0	0.0	5.191489577599131	0.0
32	5.073579587974602E-5	0.0	0.0	5.298745050088915	0.0
33	5.073579587974602E-5	0.0	0.0	5.412291761267786	0.0
34	5.073579587974602E-5	0.0	0.0	5.534311350358575	0.0
35	5.073579587974602E-5	0.0	0.0	5.675864220863066	0.0
36	1.0147159175949204E-4	0.0	0.0	5.798289696320894	0.0
37	1.0147159175949204E-4	0.0	0.0	5.915793799578386	0.0
38	1.0147159175949204E-4	0.0	0.0	6.048975263762719	0.0
39	1.0147159175949204E-4	0.0	0.0	6.217113691308197	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.0345146E-4	45.000004	36
CGGTCTA	360	0.0	41.875	31
CGTTTTT	6185	0.0	41.871464	1
TATAGCG	130	0.0	41.538464	1
CGCATCG	110	0.0	40.909092	21
CGTATGG	160	0.0	40.781254	2
CGATGAA	1980	0.0	39.886364	19
TCGATTG	80	0.0	39.375004	1
ACGTTAG	75	0.0	39.000004	1
AGTACGG	370	0.0	38.91892	2
CACGACG	410	0.0	38.414635	26
GATGAAT	2115	0.0	38.40425	20
CCGATGA	2005	0.0	38.15461	18
TCACGAC	415	0.0	37.951805	25
GGGCGAT	2790	0.0	37.580643	7
ACGTTAC	30	1.14021794E-4	37.500004	24
CGTAGAT	30	1.14021794E-4	37.500004	42
GCGATCG	30	1.14021794E-4	37.500004	33
AGGGCGA	1305	0.0	37.413795	6
TCTCACG	420	0.0	36.964287	23
>>END_MODULE