##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546845_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1381180
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.905360633661072	31.0	31.0	34.0	30.0	34.0
2	32.29939834054939	33.0	31.0	34.0	31.0	34.0
3	32.33678376460708	34.0	31.0	34.0	30.0	34.0
4	35.97073154838616	37.0	35.0	37.0	35.0	37.0
5	35.92795290983072	37.0	35.0	37.0	35.0	37.0
6	35.97742654831376	37.0	35.0	37.0	35.0	37.0
7	36.20809307983029	37.0	35.0	37.0	35.0	37.0
8	36.08079323477027	37.0	35.0	37.0	35.0	37.0
9	37.88759973356116	39.0	38.0	39.0	35.0	39.0
10	37.6175009774251	39.0	37.0	39.0	35.0	39.0
11	37.3911742133538	39.0	37.0	39.0	35.0	39.0
12	36.72601833215077	39.0	35.0	39.0	33.0	39.0
13	36.55103317453192	39.0	35.0	39.0	33.0	39.0
14	37.648804645303294	40.0	35.0	41.0	33.0	41.0
15	37.77726509216757	40.0	35.0	41.0	33.0	41.0
16	37.84732257924384	40.0	35.0	41.0	33.0	41.0
17	37.76684356854284	40.0	35.0	41.0	33.0	41.0
18	37.61641060542435	39.0	35.0	41.0	33.0	41.0
19	37.54736964045237	39.0	35.0	41.0	33.0	41.0
20	37.40162180164786	39.0	35.0	41.0	33.0	41.0
21	37.25896769429039	39.0	35.0	41.0	33.0	41.0
22	37.23019229933825	39.0	35.0	41.0	33.0	41.0
23	37.15612519729507	39.0	35.0	41.0	33.0	41.0
24	37.140309735154	39.0	35.0	41.0	33.0	41.0
25	37.02368264817041	38.0	35.0	41.0	33.0	41.0
26	36.972039125964756	38.0	35.0	41.0	33.0	41.0
27	36.913556524131536	38.0	35.0	41.0	33.0	41.0
28	36.843178296818664	38.0	35.0	41.0	33.0	41.0
29	36.72712970069071	38.0	35.0	41.0	32.0	41.0
30	36.65617008644782	38.0	35.0	41.0	32.0	41.0
31	36.44501078787703	38.0	35.0	41.0	31.0	41.0
32	36.21827567731939	38.0	35.0	41.0	31.0	41.0
33	36.03880088040661	38.0	35.0	41.0	30.0	41.0
34	35.80506885416818	38.0	35.0	41.0	30.0	41.0
35	35.655766808091634	38.0	35.0	41.0	29.0	41.0
36	35.565043658321144	38.0	35.0	41.0	28.0	41.0
37	35.306315614184975	38.0	35.0	41.0	27.0	41.0
38	35.39836371798028	38.0	35.0	41.0	27.0	41.0
39	35.309991456580605	38.0	35.0	40.0	27.0	41.0
40	35.202247353712046	37.0	35.0	40.0	27.0	41.0
41	35.13750778319987	37.0	35.0	40.0	26.0	41.0
42	35.128577013857715	37.0	35.0	40.0	27.0	41.0
43	35.01653079251075	37.0	35.0	40.0	26.0	41.0
44	34.887730780926454	37.0	34.0	40.0	26.0	41.0
45	34.83856267828958	37.0	34.0	40.0	25.0	41.0
46	34.669767879639146	36.0	34.0	40.0	24.0	41.0
47	34.64265989950622	36.0	34.0	40.0	24.0	41.0
48	34.51635051188114	36.0	34.0	40.0	24.0	41.0
49	34.49026629403843	36.0	34.0	40.0	24.0	41.0
50	34.37412936764216	35.0	34.0	40.0	24.0	41.0
51	33.84606713100393	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	0.0
13	10.0
14	15.0
15	35.0
16	79.0
17	202.0
18	534.0
19	1102.0
20	1968.0
21	3090.0
22	4708.0
23	6966.0
24	10379.0
25	15633.0
26	21623.0
27	24513.0
28	24357.0
29	24721.0
30	27090.0
31	31527.0
32	38871.0
33	51448.0
34	101985.0
35	202738.0
36	87830.0
37	112425.0
38	181255.0
39	405901.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.868561664663545	23.032262268495053	27.916057284351066	16.183118782490336
2	34.35627506914378	24.587381803964725	27.74641972805862	13.309923398832883
3	28.65535266945655	24.18381384033942	33.300511157126515	13.860322333077512
4	25.761884765200772	26.165090719529676	32.65772745044092	15.415297064828625
5	22.869068477678507	30.57595679057038	31.46164873513952	15.093325996611592
6	21.091385626782895	38.59221824816461	29.767445227993456	10.548950897059036
7	82.85545692813392	4.949608305941297	9.898058182134118	2.296876583790672
8	83.47586846030207	4.063337146497922	9.832606901345226	2.6281874918547907
9	78.46971430226328	6.045048436843858	11.735834576231918	3.749402684660942
10	46.37302885938111	24.409635239432948	17.53725075659943	11.680085144586513
11	40.30510143500485	21.50110774844698	23.50482920401396	14.688961612534209
12	35.89771065320957	20.61367815925513	28.03696838934824	15.451642798187057
13	24.453800373593594	29.818416136926395	28.854168175038737	16.873615314441277
14	18.634138924687583	32.74185841092399	31.063004097945235	17.560998566443185
15	17.610014625175573	23.037402800503916	42.53160341157561	16.820979162744898
16	20.977280296558014	20.096366874701342	40.82987011106445	18.096482717676192
17	21.339506798534586	20.842757642016245	30.109182003793855	27.708553555655307
18	24.20691003344966	23.59272506117957	32.47491275575957	19.725452149611204
19	27.922645853545518	26.18384280108313	26.92407941035926	18.96943193501209
20	29.367569759191415	24.856065103751863	26.191444996307506	19.584920140749215
21	25.53866983304131	26.733517716734966	28.79248179093239	18.93533065929133
22	23.68214135739006	24.259690988864595	29.00469164048133	23.05347601326402
23	21.736920604121114	28.296746260443967	28.6583935475463	21.307939587888615
24	23.166495315599704	23.942715648937867	33.69437727160833	19.196411763854098
25	21.910757468251784	24.736167624784606	31.67255535122142	21.68051955574219
26	19.708944525695422	28.687716300554598	28.887979843322377	22.715359330427606
27	20.1083131814825	27.5688179672454	30.736182105156463	21.586686746115642
28	18.305941296572495	27.23721745174416	34.77483021763999	19.682011034043352
29	20.068854168175037	26.044034810813944	33.48513589828987	20.401975122721154
30	21.990037504163105	25.973298194297627	31.975412328588597	20.061251972950668
31	28.47369640452367	24.480227052230703	26.663143109515055	20.382933433730578
32	28.834619673033203	25.714389145513255	26.469974948956686	18.98101623249685
33	27.15149365035694	25.63597793191329	26.70144369307404	20.511084724655728
34	22.041008413096048	26.1024631112527	29.82819038792916	22.028338087722094
35	21.11636426823441	27.298107415398427	30.350931811928934	21.234596504438233
36	26.27912364789528	27.985128658103942	26.470988574986603	19.264759119014176
37	22.40859265265932	30.64727262196093	27.048538206461142	19.895596518918605
38	22.332208691119188	32.16973891889543	24.662028120882145	20.83602426910323
39	22.361386640408927	29.432803834402467	26.5299960902996	21.675813434889008
40	23.1969040964972	26.403292836560045	27.0372435164135	23.36255955052926
41	19.30450773975876	26.064234929552992	27.597199496083057	27.03405783460519
42	22.059543289071666	27.378545881058226	25.087099436713533	25.47481139315658
43	21.354421581546216	26.937401352466733	26.569020692451385	25.13915637353567
44	21.42957471147859	28.231874194529315	27.635572481501324	22.702978612490767
45	20.8615097235697	30.7821572857991	25.275271868981598	23.081061121649604
46	23.054706844871774	29.373072300496677	26.52782403452121	21.04439682011034
47	22.130497111165816	27.067073082436753	28.177283192632384	22.62514661376504
48	23.052317583515546	24.588033420698245	29.467484324997468	22.892164670788745
49	21.876076977656787	24.152898246426968	30.061107169232105	23.90991760668414
50	20.061613982247064	27.92908961902142	29.085998928452483	22.923297470279035
51	19.433600254854543	29.204882781389824	27.713114872789934	23.648402090965696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1202.0
1	1851.0
2	2500.0
3	13763.5
4	25027.0
5	16545.0
6	8063.0
7	8191.0
8	8319.0
9	8798.0
10	9277.0
11	9604.0
12	9931.0
13	9679.0
14	9427.0
15	9126.5
16	8826.0
17	8248.0
18	7670.0
19	7574.5
20	7479.0
21	7078.5
22	6678.0
23	6696.0
24	6714.0
25	7515.0
26	8896.0
27	9476.0
28	11874.5
29	14273.0
30	15881.0
31	17489.0
32	20209.0
33	22929.0
34	25165.0
35	27401.0
36	30692.0
37	33983.0
38	40417.0
39	46851.0
40	61411.5
41	75972.0
42	87455.0
43	98938.0
44	101876.0
45	104814.0
46	106114.5
47	107415.0
48	104607.0
49	101799.0
50	99719.5
51	97640.0
52	92447.0
53	87254.0
54	80652.5
55	74051.0
56	71355.5
57	68660.0
58	68255.5
59	67851.0
60	65564.5
61	63278.0
62	57150.5
63	51023.0
64	42702.0
65	34381.0
66	28038.5
67	21696.0
68	17807.0
69	13918.0
70	11308.0
71	8698.0
72	7305.0
73	5912.0
74	4503.5
75	2248.0
76	1401.0
77	1069.0
78	737.0
79	590.5
80	444.0
81	310.5
82	177.0
83	155.5
84	134.0
85	80.0
86	26.0
87	20.5
88	15.0
89	13.5
90	12.0
91	8.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1381180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.33653051716595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5041189026779	20.14358534818089
2	6.137325811465271	3.1099708543196023
3	2.3282655169723125	1.7697051096850245
4	1.279191571361532	1.296411051404117
5	0.9341777868715628	1.183441200276495
6	0.6839484981261719	1.039732919696614
7	0.5419823905116682	0.9612367363875804
8	0.4782040739812644	0.9692825691087512
9	0.39501113724450937	0.9007390560074346
>10	6.165726235750643	38.54626685065893
>50	1.4300750088918315	24.047305732872445
>100	0.11878612142448086	4.268166612971685
>500	0.0020280557314600635	0.33737197956753284
>1k	8.691667420543131E-4	0.288483872867799
>5k	0.0	0.0
>10k+	2.8972224735143763E-4	1.138300105995155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15507	1.122735631851026	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1711	0.12387958122764592	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.172055778392389E-4	0.0	0.0	0.018896885272013785	0.0
2	2.172055778392389E-4	0.0	0.0	0.060455552498588166	0.0
3	2.172055778392389E-4	0.0	0.0	0.11048523726089286	0.0
4	2.172055778392389E-4	0.0	0.0	0.18245268538496068	0.0
5	2.172055778392389E-4	0.0	0.0	0.36222650197657075	0.0
6	2.172055778392389E-4	0.0	0.0	0.7052665112440087	0.0
7	2.172055778392389E-4	0.0	0.0	0.9532428792771398	0.0
8	2.172055778392389E-4	0.0	0.0	1.5703239259184176	0.0
9	2.172055778392389E-4	0.0	0.0	1.9427590900534326	0.0
10	2.172055778392389E-4	0.0	0.0	2.4098958861263555	0.0
11	2.172055778392389E-4	0.0	0.0	2.731432543187709	0.0
12	2.172055778392389E-4	0.0	0.0	2.9955545258402236	0.0
13	2.172055778392389E-4	0.0	0.0	3.1196513126457086	0.0
14	2.172055778392389E-4	0.0	0.0	3.1705498197193704	0.0
15	2.172055778392389E-4	0.0	0.0	3.226661260661174	0.0
16	2.172055778392389E-4	0.0	0.0	3.3325851807874427	0.0
17	2.172055778392389E-4	0.0	0.0	3.4373506711652357	0.0
18	2.172055778392389E-4	0.0	0.0	3.613287189215019	0.0
19	2.172055778392389E-4	0.0	0.0	3.700314224069274	0.0
20	2.896074371189852E-4	0.0	0.0	3.8064553497733824	0.0
21	2.896074371189852E-4	0.0	0.0	3.93265179049798	0.0
22	2.896074371189852E-4	0.0	0.0	4.061671903734488	0.0
23	2.896074371189852E-4	0.0	0.0	4.2106025282729265	0.0
24	2.896074371189852E-4	0.0	0.0	4.323477026890051	0.0
25	2.896074371189852E-4	0.0	0.0	4.4194818922949946	0.0
26	2.896074371189852E-4	0.0	0.0	4.515197150262819	7.24018592797463E-5
27	2.896074371189852E-4	0.0	0.0	4.60902995988937	1.448037185594926E-4
28	2.896074371189852E-4	0.0	0.0	4.712564618659407	1.448037185594926E-4
29	2.896074371189852E-4	0.0	0.0	4.820950202001187	1.448037185594926E-4
30	2.896074371189852E-4	0.0	0.0	4.978858657090314	1.448037185594926E-4
31	2.896074371189852E-4	0.0	0.0	5.104548284799954	1.448037185594926E-4
32	3.620092963987315E-4	0.0	0.0	5.227052230701284	1.448037185594926E-4
33	3.620092963987315E-4	0.0	0.0	5.3590408201682616	1.448037185594926E-4
34	3.620092963987315E-4	0.0	0.0	5.493273867272912	1.448037185594926E-4
35	3.620092963987315E-4	0.0	0.0	5.648286247990848	1.448037185594926E-4
36	3.620092963987315E-4	0.0	0.0	5.785560173185248	1.448037185594926E-4
37	3.620092963987315E-4	0.0	0.0	5.9292778638555435	1.448037185594926E-4
38	3.620092963987315E-4	0.0	0.0	6.121287594665431	1.448037185594926E-4
39	3.620092963987315E-4	0.0	0.0	6.357752067073083	1.448037185594926E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	20	7.033781E-4	45.0	28
TACGACG	20	7.033781E-4	45.0	1
GTCGTTT	20	7.033781E-4	45.0	9
CGTTTTT	8270	0.0	43.612453	1
GTATGCG	70	0.0	41.785717	1
CGACGGT	195	0.0	40.384617	28
ATAACGG	45	1.9288564E-8	40.0	2
ATGGGAC	625	0.0	39.600002	5
CGTAAGG	200	0.0	39.375004	2
CGTATGG	135	0.0	38.333336	2
ATAACGC	100	0.0	38.250004	11
GTTTTTT	10045	0.0	37.89945	2
CTCACGA	215	0.0	37.67442	24
TATGCGG	275	0.0	37.636364	2
CGTGGTA	30	1.14004084E-4	37.500004	20
TTAGCCG	30	1.14004084E-4	37.500004	1
GGGACCG	600	0.0	37.500004	7
TACGACT	30	1.14004084E-4	37.500004	13
AATGTCG	30	1.14004084E-4	37.500004	15
TACCGTT	30	1.14004084E-4	37.500004	34
>>END_MODULE