FastQCFastQC Report
Thu 26 May 2016
SRR1546844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546844_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4525372
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396000.875066182404452No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59310.13106104868284862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220000.043.251141
CGGTCTA5900.041.94915431
ACGGGAT9250.038.189195
GGGCGAT64600.037.8250737
AGGGCGA35100.037.8205156
ACGGGTA4050.037.777785
GCGATAT4950.037.7272729
CGTAAGG6750.037.6666682
TAGGGCG13750.037.6363685
TACGGGA14350.037.630664
GCGTAAG2300.037.1739121
TCACGAC6600.037.15909225
TTACGGG15350.036.4983673
ACGGTCT7000.035.6785730
TAAGGGC24050.035.6444934
GTTTTTT284000.035.5801052
TAGGGTA20900.035.418665
TTAGACG2550.035.2941171
CACGACG6950.035.28776626
TAGGGAC28650.035.1832475
Produced by FastQC (version 0.11.5)