##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546844_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 4525372 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915874982211406 33.0 31.0 34.0 30.0 34.0 2 32.312151354628966 33.0 31.0 34.0 31.0 34.0 3 32.36117163406677 34.0 31.0 34.0 30.0 34.0 4 35.98289731761278 37.0 35.0 37.0 35.0 37.0 5 35.940758019451216 37.0 35.0 37.0 35.0 37.0 6 35.986575910223515 37.0 35.0 37.0 35.0 37.0 7 36.22009218247693 37.0 35.0 37.0 35.0 37.0 8 36.08456498161919 37.0 35.0 37.0 35.0 37.0 9 37.90063711889321 39.0 38.0 39.0 35.0 39.0 10 37.62229624437505 39.0 37.0 39.0 35.0 39.0 11 37.40755831785763 39.0 37.0 39.0 35.0 39.0 12 36.87258196674218 39.0 35.0 39.0 33.0 39.0 13 36.725262144195 39.0 35.0 39.0 33.0 39.0 14 37.88482560107766 40.0 35.0 41.0 33.0 41.0 15 37.99950987454733 40.0 35.0 41.0 33.0 41.0 16 38.07315243918069 40.0 35.0 41.0 34.0 41.0 17 38.0060534250002 40.0 35.0 41.0 34.0 41.0 18 37.89417842334288 40.0 36.0 41.0 33.0 41.0 19 37.83017904384435 40.0 36.0 41.0 34.0 41.0 20 37.685756662656686 40.0 35.0 41.0 34.0 41.0 21 37.558526680237556 40.0 35.0 41.0 33.0 41.0 22 37.52120709634479 39.0 35.0 41.0 33.0 41.0 23 37.43388676113256 39.0 35.0 41.0 33.0 41.0 24 37.41638963603434 39.0 35.0 41.0 33.0 41.0 25 37.31472860131719 39.0 35.0 41.0 33.0 41.0 26 37.269115555583056 39.0 35.0 41.0 33.0 41.0 27 37.199315989934085 39.0 35.0 41.0 33.0 41.0 28 37.13825051288601 39.0 35.0 41.0 33.0 41.0 29 37.041322348748345 39.0 35.0 41.0 33.0 41.0 30 36.98427422099222 39.0 35.0 41.0 32.0 41.0 31 36.78700204093719 39.0 35.0 41.0 32.0 41.0 32 36.5992943784511 39.0 35.0 41.0 31.0 41.0 33 36.44360286844927 39.0 35.0 41.0 31.0 41.0 34 36.237949719934626 39.0 35.0 41.0 30.0 41.0 35 36.094064753129686 39.0 35.0 41.0 30.0 41.0 36 36.00881783862189 39.0 35.0 41.0 30.0 41.0 37 35.763872892659435 38.0 35.0 41.0 29.0 41.0 38 35.84774997502968 38.0 35.0 41.0 29.0 41.0 39 35.74382857365096 38.0 35.0 41.0 29.0 41.0 40 35.63888670367872 38.0 35.0 41.0 28.0 41.0 41 35.56003130792342 38.0 35.0 41.0 28.0 41.0 42 35.55992700710571 38.0 35.0 41.0 28.0 41.0 43 35.43778213150212 38.0 35.0 40.0 28.0 41.0 44 35.29327798908024 38.0 35.0 40.0 27.0 41.0 45 35.23543368368391 37.0 35.0 40.0 27.0 41.0 46 35.05767702633065 37.0 34.0 40.0 26.0 41.0 47 35.0264088786513 37.0 34.0 40.0 26.0 41.0 48 34.88669483967285 37.0 34.0 40.0 26.0 41.0 49 34.85904760978766 36.0 34.0 40.0 26.0 41.0 50 34.7362627867941 36.0 34.0 40.0 26.0 41.0 51 34.16955556360892 36.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 5.0 11 8.0 12 12.0 13 22.0 14 52.0 15 107.0 16 300.0 17 707.0 18 1534.0 19 3006.0 20 5506.0 21 9123.0 22 13945.0 23 20354.0 24 29236.0 25 44304.0 26 62009.0 27 72629.0 28 74983.0 29 76388.0 30 83695.0 31 99021.0 32 120737.0 33 160490.0 34 305647.0 35 575473.0 36 288767.0 37 384219.0 38 633998.0 39 1458487.0 40 603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.96074400071419 22.187767105113128 28.3123022814478 15.539186612724876 2 33.51333326851362 24.407650906930968 28.991804430663382 13.087211393892037 3 28.13326727614879 25.370400488622813 32.87676681607611 13.619565419152282 4 25.3263599103013 26.596487537378145 32.685710699584476 15.391441852736085 5 23.695068604304794 30.659357948915584 30.453872079466613 15.191701367313007 6 20.9112532627152 39.81195358083269 28.56448044492254 10.712312711529572 7 84.03110285740046 4.670113307812042 9.06979580905172 2.228988025735785 8 84.6072985823044 4.175855598169608 8.598983685761082 2.617862133764915 9 79.73729010565319 5.531898814064347 10.644229910822801 4.086581169459659 10 44.88391230599385 24.680711331576717 17.32520553006471 13.11017083236472 11 37.797865015295976 23.26157937955156 23.253535841915316 15.687019763237146 12 32.32932010893248 21.729174971692935 28.91139115193182 17.030113767442764 13 23.675247029415484 29.039380629923905 29.581656491444242 17.703715849216373 14 18.74855812958581 32.375327376401316 30.9369041926277 17.939210301385167 15 18.195034573953254 23.355450115482217 40.97884107649051 17.470674234074014 16 20.66104178838778 20.81236636457732 38.74375410463494 19.782837742399963 17 21.084631274511796 21.026912262682494 30.19102076028225 27.69743570252346 18 23.4122631244459 23.204589589540923 32.561875576195725 20.821271709817445 19 26.57262209603984 24.998652044517 27.8535775622424 20.57514829720076 20 28.831022068461998 24.13870064162681 26.637301861592817 20.39297542831838 21 25.534652178870598 25.702218513748704 28.75653095480327 20.006598352577424 22 23.499990718995033 23.2944827519152 29.376723062766995 23.828803466322768 23 22.347731854972363 26.652350348214465 28.037429851070804 22.96248794574236 24 22.251297793860925 24.587216255370826 32.98984039323176 20.171645557536486 25 21.765591867364716 24.480727772214088 31.72859601376417 22.025084346657025 26 20.251572688388933 27.950762942803376 28.863328804792182 22.93433556401551 27 20.64782740512824 27.163424354948056 30.789778166303233 21.398970073620465 28 19.110804592418038 26.15298808584134 34.784366898456085 19.95184042328454 29 20.209609287369084 25.17572036066869 33.642736994881304 20.97193335708092 30 22.53995914590005 25.71399655100177 31.402081420046795 20.343962883051383 31 26.889988270577536 24.3755430492786 27.378257522254522 21.35621115788934 32 27.633684037466978 25.805259766489915 27.4180774530801 19.14297874296301 33 26.391023765560046 25.369494485757194 27.017314819643556 21.222166929039204 34 22.562520827017092 26.21039773083848 29.234524808126274 21.992556634018154 35 22.254568243229507 25.638245872383532 29.552929571314802 22.554256313072162 36 26.169229844529905 25.943414154681648 27.376688590462837 20.510667410325603 37 22.575249062397525 28.21593451322897 28.275951678668626 20.932864745704883 38 22.22745445015349 29.495188461854628 25.518896568061145 22.758460519930736 39 21.909138077488436 27.557844968325256 27.578683034234537 22.954333919951775 40 23.138009427733234 25.212645501850456 28.087591473142982 23.56175359727333 41 20.30149123652155 24.778382860016812 28.621845894657945 26.298280008803697 42 22.832089825985577 26.458576223125966 26.643356612450862 24.06597733843759 43 22.70447600771826 25.914355770089177 27.315831715050166 24.065336507142394 44 22.008179659042394 27.500391128066376 28.04839027598173 22.443038936909495 45 20.81970277802576 29.427702297181312 26.37948880224653 23.37310612254639 46 23.289952737587097 27.57086047290698 27.78419100131437 21.354995788191555 47 22.65829195920247 26.477315014102707 28.504529572375485 22.359863454319335 48 23.063672997490592 24.984023412881857 29.346184136906313 22.606119452721234 49 21.956206031238978 24.299239929888635 30.237447882737595 23.50710615613479 50 21.16153102993522 26.86722771078267 29.433359290683725 22.537881968598384 51 20.982297145958388 28.156712862500587 27.53382484357087 23.327165147970156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3376.0 1 5534.5 2 7693.0 3 37864.5 4 68036.0 5 45881.5 6 23727.0 7 23639.0 8 23551.0 9 24354.0 10 25157.0 11 24743.5 12 24330.0 13 23408.0 14 22486.0 15 21818.5 16 21151.0 17 19731.0 18 18311.0 19 19021.0 20 19731.0 21 18950.5 22 18170.0 23 20014.0 24 21858.0 25 24320.5 26 32863.5 27 38944.0 28 44484.5 29 50025.0 30 60589.0 31 71153.0 32 76609.5 33 82066.0 34 90911.5 35 99757.0 36 112034.0 37 124311.0 38 139950.5 39 155590.0 40 194862.5 41 234135.0 42 267298.5 43 300462.0 44 316531.5 45 332601.0 46 347686.5 47 362772.0 48 359451.0 49 356130.0 50 351245.0 51 346360.0 52 323566.5 53 300773.0 54 281459.5 55 262146.0 56 246902.0 57 231658.0 58 222419.5 59 213181.0 60 198105.5 61 183030.0 62 166098.5 63 149167.0 64 128674.5 65 108182.0 66 89742.0 67 71302.0 68 61291.0 69 51280.0 70 41255.0 71 31230.0 72 25973.5 73 20717.0 74 16459.0 75 8993.0 76 5785.0 77 4391.5 78 2998.0 79 2371.0 80 1744.0 81 1240.5 82 737.0 83 518.0 84 299.0 85 208.0 86 117.0 87 107.5 88 98.0 89 62.5 90 27.0 91 21.5 92 16.0 93 11.5 94 7.0 95 6.0 96 5.0 97 5.5 98 6.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4525372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.161419987861972 #Duplication Level Percentage of deduplicated Percentage of total 1 81.1707102625248 15.553460700532959 2 7.3923473243110935 2.8329574355454517 3 2.343586529836373 1.3471933732827226 4 1.1556638419806051 0.8857664096390612 5 0.7327965165464565 0.7020710909594448 6 0.4684653339822533 0.538587660851279 7 0.37759812963575196 0.5064721444007256 8 0.29952996613102634 0.4591535583989334 9 0.24458447622666954 0.42179272843414123 >10 3.0178957927700796 13.939558185962062 >50 1.4007836944203658 19.67073873843371 >100 1.3876590128373743 40.26407017224129 >500 0.00436658303511791 0.5736139138119091 >1k 0.0037765042465884475 1.2749488473040818 >5k 1.1801575770588899E-4 0.13412063876127187 >10k+ 1.1801575770588899E-4 0.8954944014409655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39600 0.875066182404452 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 5931 0.13106104868284862 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2097630868799294E-5 0.0 0.0 0.03259400553147896 0.0 2 2.2097630868799294E-5 0.0 0.0 0.11320616294085878 0.0 3 2.2097630868799294E-5 0.0 0.0 0.17313493785704248 0.0 4 2.2097630868799294E-5 0.0 0.0 0.2681326529620106 0.0 5 2.2097630868799294E-5 0.0 0.0 0.5316910963341798 0.0 6 2.2097630868799294E-5 0.0 0.0 0.8358428876123333 0.0 7 2.2097630868799294E-5 0.0 0.0 1.0702987511302937 0.0 8 2.2097630868799294E-5 0.0 0.0 1.6262972414201529 0.0 9 2.2097630868799294E-5 0.0 0.0 1.9353989020129174 0.0 10 2.2097630868799294E-5 0.0 0.0 2.460085049361688 0.0 11 2.2097630868799294E-5 0.0 0.0 2.775086777396422 0.0 12 2.2097630868799294E-5 0.0 0.0 3.0931600761219187 0.0 13 2.2097630868799294E-5 0.0 0.0 3.217326663973702 0.0 14 2.2097630868799294E-5 0.0 0.0 3.2677976528780395 0.0 15 2.2097630868799294E-5 0.0 0.0 3.3377145569469207 0.0 16 7.955147112767746E-4 0.0 0.0 3.4676265288245918 0.0 17 7.955147112767746E-4 0.0 0.0 3.6018696363525473 0.0 18 8.176123421455739E-4 0.0 0.0 3.8235088739666043 0.0 19 8.176123421455739E-4 0.0 0.0 3.9280748632377627 0.0 20 8.176123421455739E-4 0.0 0.0 4.040065656480837 0.0 21 8.176123421455739E-4 0.0 0.0 4.170419580975884 0.0 22 8.176123421455739E-4 0.0 0.0 4.308596066798486 0.0 23 8.176123421455739E-4 0.0 0.0 4.460959231638857 0.0 24 8.176123421455739E-4 0.0 0.0 4.582871861142023 0.0 25 8.176123421455739E-4 0.0 0.0 4.687128483581018 0.0 26 8.176123421455739E-4 0.0 0.0 4.7921806207312905 0.0 27 8.397099730143732E-4 0.0 0.0 4.907044105987309 0.0 28 8.618076038831724E-4 0.0 0.0 5.019918804465136 0.0 29 8.839052347519718E-4 0.0 0.0 5.138317910660162 0.0 30 8.839052347519718E-4 0.0 0.0 5.300160959143248 0.0 31 8.839052347519718E-4 0.0 0.0 5.431310398349572 0.0 32 8.839052347519718E-4 0.0 0.0 5.556603965375664 0.0 33 8.839052347519718E-4 0.0 0.0 5.68846052876979 0.0 34 8.839052347519718E-4 0.0 0.0 5.824648227814199 0.0 35 8.839052347519718E-4 0.0 0.0 5.983773267700423 0.0 36 8.839052347519718E-4 0.0 0.0 6.116933591315808 0.0 37 8.839052347519718E-4 0.0 0.0 6.260877558795166 0.0 38 8.839052347519718E-4 0.0 0.0 6.417129906668446 0.0 39 8.839052347519718E-4 0.0 0.0 6.634128641800055 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 22000 0.0 43.25114 1 CGGTCTA 590 0.0 41.949154 31 ACGGGAT 925 0.0 38.18919 5 GGGCGAT 6460 0.0 37.825073 7 AGGGCGA 3510 0.0 37.820515 6 ACGGGTA 405 0.0 37.77778 5 GCGATAT 495 0.0 37.727272 9 CGTAAGG 675 0.0 37.666668 2 TAGGGCG 1375 0.0 37.636368 5 TACGGGA 1435 0.0 37.63066 4 GCGTAAG 230 0.0 37.173912 1 TCACGAC 660 0.0 37.159092 25 TTACGGG 1535 0.0 36.498367 3 ACGGTCT 700 0.0 35.67857 30 TAAGGGC 2405 0.0 35.644493 4 GTTTTTT 28400 0.0 35.580105 2 TAGGGTA 2090 0.0 35.41866 5 TTAGACG 255 0.0 35.294117 1 CACGACG 695 0.0 35.287766 26 TAGGGAC 2865 0.0 35.183247 5 >>END_MODULE