FastQCFastQC Report
Thu 26 May 2016
SRR1546843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546843_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4788354
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1073442.241772433700599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT606900.043.7839851
CGGTCTA6800.041.0294131
CACGACG6950.040.14388326
GTTTTTT703550.038.536712
TCACGAC7250.038.4827625
ACGGGAT10950.036.7808235
GGGCGAT64000.036.6328127
TACGGGA12050.036.410794
ACGGTCT7800.035.4807730
ATAGGGA43400.035.460834
TAGGGAC33950.035.390285
TAGGGAT38300.035.0130545
GTAGGGA48000.034.968754
AGGGCGA35300.034.737966
TAAGGGA45150.034.534884
CGTTAGG8350.034.491022
AGGGATT43150.034.4669766
CGACGGT8500.034.41176628
ATAGCGG8000.034.31252
GGCGATA14300.034.30078
Produced by FastQC (version 0.11.5)