FastQCFastQC Report
Thu 26 May 2016
SRR1546842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546842_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1112419
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89010.8001481456177933No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12030.10814270522168355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG302.1654396E-645.0000041
GCGATAC302.1654396E-645.0000049
TATCGAT207.0331874E-445.00000411
TCGAGTA253.8907252E-545.030
CCCGATA253.8907252E-545.016
CGGTCTA1200.043.12500431
CGTTTTT51750.042.6521721
TCTCACG1300.039.8076923
GTGTACG403.4580808E-739.3750041
TACGGGA2650.039.0566024
AGGGCGA9650.038.2383426
GACCGAT3850.037.987019
GGGCGAT16800.037.7678537
CGATACG301.1398982E-437.50000410
TGTAGCG900.037.51
ATAGGGC4750.037.421054
GTTTGCG850.037.0588261
ACGATGG850.037.0588262
AAGGGCG4200.036.9642835
CTCACGA1350.036.66666424
Produced by FastQC (version 0.11.5)