FastQCFastQC Report
Thu 26 May 2016
SRR1546841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546841_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3242818
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288530.8897508278293756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46250.14262286690156525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150200.043.0076521
CGGTCTA3800.040.26315731
TAGTACG1350.040.01
TACGGGA10050.037.835824
ACGGGAT7400.037.70275
GTTACGG2800.036.9642832
ATACGGG8000.036.843753
CGCATCG1650.036.8181821
GGGCGAT40900.036.4731037
ATGGGAC21000.036.3214265
TAGGGAC29250.036.0769235
GTTTTTT191650.036.0540052
CGCGAAA250.002107673336.042
TAATCGG1000.036.02
ATAGGGA34800.035.9482774
TAACGGG7350.035.8163263
AGGGCGA23000.035.8043486
ACGACGG4350.035.68965527
TATGGGA24850.035.674044
CGTAAGG5300.035.6603782
Produced by FastQC (version 0.11.5)