##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546841_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3242818
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938226258766296	33.0	31.0	34.0	30.0	34.0
2	32.33188942456839	34.0	31.0	34.0	31.0	34.0
3	32.39262548807858	34.0	31.0	34.0	30.0	34.0
4	35.99689961015388	37.0	35.0	37.0	35.0	37.0
5	35.95351635521944	37.0	35.0	37.0	35.0	37.0
6	36.00741052997732	37.0	35.0	37.0	35.0	37.0
7	36.23633210374434	37.0	35.0	37.0	35.0	37.0
8	36.105968019173446	37.0	35.0	37.0	35.0	37.0
9	37.91329269789424	39.0	39.0	39.0	35.0	39.0
10	37.64982925344562	39.0	37.0	39.0	35.0	39.0
11	37.42573403749455	39.0	37.0	39.0	35.0	39.0
12	36.91616612464838	39.0	35.0	39.0	33.0	39.0
13	36.78383369032736	39.0	35.0	39.0	33.0	39.0
14	37.926811803807674	40.0	36.0	41.0	33.0	41.0
15	38.049422755146914	40.0	36.0	41.0	33.0	41.0
16	38.125339442423225	40.0	36.0	41.0	34.0	41.0
17	38.069923443128786	40.0	36.0	41.0	34.0	41.0
18	37.95936743906072	40.0	36.0	41.0	34.0	41.0
19	37.88377916984549	40.0	36.0	41.0	34.0	41.0
20	37.764175171101186	40.0	35.0	41.0	34.0	41.0
21	37.627726255374185	40.0	35.0	41.0	33.0	41.0
22	37.591793002259145	40.0	35.0	41.0	33.0	41.0
23	37.49970797004334	39.0	35.0	41.0	33.0	41.0
24	37.49767794553996	39.0	35.0	41.0	33.0	41.0
25	37.385405533088814	39.0	35.0	41.0	33.0	41.0
26	37.35777555200446	39.0	35.0	41.0	33.0	41.0
27	37.30168544765694	39.0	35.0	41.0	33.0	41.0
28	37.23204817538326	39.0	35.0	41.0	33.0	41.0
29	37.13057747921715	39.0	35.0	41.0	33.0	41.0
30	37.071713552842006	39.0	35.0	41.0	33.0	41.0
31	36.888384732044784	39.0	35.0	41.0	32.0	41.0
32	36.738973633426234	39.0	35.0	41.0	31.0	41.0
33	36.59205974556697	39.0	35.0	41.0	31.0	41.0
34	36.403851835039774	39.0	35.0	41.0	31.0	41.0
35	36.27294377914517	39.0	35.0	41.0	30.0	41.0
36	36.19089507952651	39.0	35.0	41.0	30.0	41.0
37	35.929096853415764	39.0	35.0	41.0	29.0	41.0
38	36.011246391256	39.0	35.0	41.0	30.0	41.0
39	35.90541405653971	38.0	35.0	41.0	29.0	41.0
40	35.79115818402389	38.0	35.0	41.0	29.0	41.0
41	35.721221789196925	38.0	35.0	41.0	29.0	41.0
42	35.71267336002205	38.0	35.0	41.0	29.0	41.0
43	35.61298722284137	38.0	35.0	41.0	29.0	41.0
44	35.487115527297554	38.0	35.0	40.0	28.0	41.0
45	35.44932925622098	38.0	35.0	40.0	28.0	41.0
46	35.27847692963342	38.0	35.0	40.0	27.0	41.0
47	35.257960822963234	37.0	35.0	40.0	27.0	41.0
48	35.12071136893899	37.0	35.0	40.0	27.0	41.0
49	35.08720995134479	37.0	35.0	40.0	27.0	41.0
50	34.973188442891335	37.0	34.0	40.0	27.0	41.0
51	34.42938795825113	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	10.0
12	11.0
13	27.0
14	54.0
15	121.0
16	201.0
17	530.0
18	997.0
19	2108.0
20	3653.0
21	5922.0
22	8795.0
23	12973.0
24	19517.0
25	30279.0
26	42369.0
27	50089.0
28	51197.0
29	52248.0
30	58384.0
31	68404.0
32	84825.0
33	112469.0
34	213449.0
35	407508.0
36	202496.0
37	272363.0
38	452319.0
39	1089086.0
40	409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.90295107526848	22.916549741613622	28.154278161771646	15.026221021346247
2	33.34923514054751	24.439576935862572	28.85391656269331	13.357271360896602
3	29.500360488932774	24.4239423859125	32.367188044472435	13.708509080682296
4	26.252321283525625	26.147659227252344	31.980919064838055	15.619100424383978
5	23.33677067291473	30.750754436419186	30.478090352280024	15.434384538386059
6	21.26425226454275	39.48257349009411	28.448559246926592	10.804614998436545
7	83.8421089311827	4.918006499285498	8.783656683785521	2.4562278857462863
8	84.4981741189299	3.9217125352085747	8.84912443436542	2.7309889114961123
9	79.43702051734016	5.837947118833064	10.762398629833681	3.962633733993089
10	44.516127639602345	27.274271944956517	16.054184971219478	12.155415444221662
11	35.70999050825547	23.159455757307377	25.117043262989164	16.01351047144798
12	32.339280218624666	21.784201271856762	29.285547323346545	16.590971186172027
13	23.69920236041616	29.733830267378558	29.33223511156038	17.234732260644908
14	17.897643346003385	32.51298716116662	31.74754179852215	17.841827694307852
15	17.69905064052315	24.025430967757057	40.97164873267633	17.30386965904346
16	21.025879343213216	21.828884630589815	38.36838823517077	18.7768477910262
17	21.276155491920914	21.550577306527842	29.901123035582017	27.272144165969227
18	23.909297407378396	23.642153213655533	32.27017365760274	20.178375721363334
19	26.796693493128508	25.502232934441587	27.51875066685827	20.182322905571638
20	27.99185769907531	24.85646743048793	26.9631228147864	20.188552055650362
21	24.90956939304025	25.858589658747423	29.704103036309775	19.52773791190255
22	23.852679983890553	23.395824249156135	29.651556146536745	23.099939620416563
23	21.373601602063392	26.835240213912716	28.963420087097084	22.827738096926808
24	21.748337402839134	24.94120854145993	33.47079607921258	19.83965797648835
25	21.581784731674738	25.298367037558073	31.641769596690285	21.478078634076905
26	19.625584907941178	28.753294202758216	28.91676313625988	22.704357753040718
27	20.291980616858545	27.50552143228513	31.14109394976838	21.061404001087944
28	18.571594212194455	26.563285389435976	34.633951088220186	20.231169310149383
29	20.212666884172965	25.552497858344193	33.56336988384794	20.671465373634906
30	21.383962960610187	26.044199828667537	32.011293880816005	20.560543329906274
31	25.960414676370984	25.226022551990273	27.850375815108958	20.963186956529782
32	26.05234089609716	25.846470569732865	28.002743293024768	20.098445241145203
33	25.14088055512212	25.75257075790254	27.99127178891939	21.115276898055953
34	21.082620116207572	26.458253284643174	30.211100345440293	22.248026253708968
35	20.901512203275054	26.34862641073289	30.50553561747838	22.244325768513683
36	25.968555743800607	25.652071747473958	27.91279066540275	20.46658184332269
37	22.342018577669176	28.580481544138465	28.203802988635196	20.87369688955717
38	22.130782547771723	29.45746569804411	26.097918538752406	22.313833215431764
39	22.358917460060972	27.412485067000368	27.305047646830626	22.923549826108033
40	22.671053386283166	25.62533574193803	28.112401004311682	23.59120986746712
41	20.21692244214754	25.162343369254764	28.646751066510674	25.973983122087024
42	22.591153743441662	26.688454301166452	26.438856574744555	24.281535380647327
43	22.256969092930902	26.61021987666283	27.2848491651397	23.84796186526657
44	21.245811513319588	28.362677153019376	28.23726154227588	22.154249791385148
45	21.070686051452782	29.576744670838757	26.5009938886487	22.85157538905976
46	22.884108821401632	28.03595514765244	28.206578352531658	20.873357678414266
47	22.144165969228	26.94156748852387	28.46228187952577	22.45198466272236
48	22.345503201228066	25.926431887327627	29.351385122445972	22.376679788998334
49	21.77861970668721	24.778788078763593	30.302810703530074	23.13978151101912
50	20.891520893247787	27.756876889174787	28.87550272633247	22.47609949124496
51	20.665390410439315	28.75384927553751	27.63004275910643	22.950717554916743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2389.0
1	4082.0
2	5775.0
3	27011.0
4	48247.0
5	32387.0
6	16527.0
7	16658.0
8	16789.0
9	17269.0
10	17749.0
11	17389.5
12	17030.0
13	16202.0
14	15374.0
15	14879.0
16	14384.0
17	13859.5
18	13335.0
19	13264.0
20	13193.0
21	13487.0
22	13781.0
23	14837.5
24	15894.0
25	18339.5
26	26889.5
27	32994.0
28	37859.0
29	42724.0
30	48263.0
31	53802.0
32	59216.5
33	64631.0
34	73459.0
35	82287.0
36	89911.0
37	97535.0
38	114746.5
39	131958.0
40	154268.0
41	176578.0
42	206293.0
43	236008.0
44	236616.0
45	237224.0
46	239646.0
47	242068.0
48	243680.5
49	245293.0
50	246374.0
51	247455.0
52	231677.0
53	215899.0
54	196479.0
55	177059.0
56	168000.0
57	158941.0
58	152599.0
59	146257.0
60	133403.5
61	120550.0
62	110449.0
63	100348.0
64	88687.0
65	77026.0
66	61649.0
67	46272.0
68	38399.5
69	30527.0
70	25394.0
71	20261.0
72	16522.0
73	12783.0
74	10425.5
75	5845.0
76	3622.0
77	2684.5
78	1747.0
79	1318.5
80	890.0
81	606.0
82	322.0
83	292.5
84	263.0
85	187.5
86	112.0
87	75.5
88	39.0
89	27.5
90	16.0
91	9.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3242818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.959874043342822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24638780343386	16.216676670331243
2	6.670804314250366	2.6629682776045036
3	2.161205210877182	1.2941215137277413
4	1.1173547428663635	0.8920903971737728
5	0.6850060225140931	0.6836316964156277
6	0.4831494844494468	0.5786161712230187
7	0.39092289778365286	0.5461940260294204
8	0.33757213368270467	0.5390317815079416
9	0.2638123104076144	0.4739094438137316
>10	3.715882513814465	18.466018778585333
>50	1.8753736319122045	27.297715780497533
>100	1.0460466779044282	28.09561185556864
>500	0.0044269066073990195	0.6382468135074032
>1k	0.001897245688885294	0.7046434165046628
>5k	0.0	0.0
>10k+	1.5810380740710785E-4	0.9105233775094735
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28853	0.8897508278293756	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4625	0.14262286690156525	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.251212988209637E-5	0.0	0.0	0.02423817802910925	0.0
2	9.251212988209637E-5	0.0	0.0	0.08569706964744861	0.0
3	9.251212988209637E-5	0.0	0.0	0.1332791417834735	0.0
4	9.251212988209637E-5	0.0	0.0	0.20735052044240534	0.0
5	9.251212988209637E-5	0.0	0.0	0.37936140726984985	0.0
6	9.251212988209637E-5	0.0	0.0	0.6497743629152176	0.0
7	9.251212988209637E-5	0.0	0.0	0.8524684394868908	0.0
8	9.251212988209637E-5	0.0	0.0	1.3328530925879898	0.0
9	9.251212988209637E-5	0.0	0.0	1.5890500176081421	0.0
10	9.251212988209637E-5	0.0	0.0	1.9498781615249452	0.0
11	9.251212988209637E-5	0.0	0.0	2.2064451350646257	0.0
12	1.2334950650946184E-4	0.0	0.0	2.4372012243672017	0.0
13	1.2334950650946184E-4	0.0	0.0	2.5319644827430956	0.0
14	1.2334950650946184E-4	0.0	0.0	2.5754143464110535	0.0
15	1.2334950650946184E-4	0.0	0.0	2.6251241975343667	0.0
16	1.8502425976419274E-4	0.0	0.0	2.725808232222715	0.0
17	1.8502425976419274E-4	0.0	0.0	2.843082775536586	0.0
18	1.8502425976419274E-4	0.0	0.0	3.0240365015859663	0.0
19	1.8502425976419274E-4	0.0	0.0	3.11201553710384	0.0
20	1.8502425976419274E-4	0.0	0.0	3.2094924846229422	0.0
21	1.8502425976419274E-4	0.0	0.0	3.3285247584045727	0.0
22	1.8502425976419274E-4	0.0	0.0	3.458658487772055	0.0
23	1.8502425976419274E-4	0.0	0.0	3.598814364543431	0.0
24	1.8502425976419274E-4	0.0	0.0	3.7076086292847763	0.0
25	1.8502425976419274E-4	0.0	0.0	3.800059084413618	0.0
26	1.8502425976419274E-4	0.0	0.0	3.897505194556093	0.0
27	1.8502425976419274E-4	0.0	0.0	3.9976341564651485	0.0
28	1.8502425976419274E-4	0.0	0.0	4.103838081569795	0.0
29	1.8502425976419274E-4	0.0	0.0	4.226200792027181	0.0
30	1.8502425976419274E-4	0.0	0.0	4.382268755138278	0.0
31	1.8502425976419274E-4	0.0	0.0	4.513019232038308	0.0
32	1.8502425976419274E-4	0.0	0.0	4.639298289327369	0.0
33	1.8502425976419274E-4	0.0	0.0	4.769586205577988	0.0
34	1.8502425976419274E-4	0.0	0.0	4.898023879230966	0.0
35	1.8502425976419274E-4	0.0	0.0	5.052549973510693	0.0
36	1.8502425976419274E-4	0.0	0.0	5.190084673268744	0.0
37	2.158616363915582E-4	0.0	0.0	5.327064300247501	0.0
38	2.466990130189237E-4	0.0	0.0	5.493185248139119	0.0
39	2.466990130189237E-4	0.0	0.0	5.732791664533748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15020	0.0	43.007652	1
CGGTCTA	380	0.0	40.263157	31
TAGTACG	135	0.0	40.0	1
TACGGGA	1005	0.0	37.83582	4
ACGGGAT	740	0.0	37.7027	5
GTTACGG	280	0.0	36.964283	2
ATACGGG	800	0.0	36.84375	3
CGCATCG	165	0.0	36.81818	21
GGGCGAT	4090	0.0	36.473103	7
ATGGGAC	2100	0.0	36.321426	5
TAGGGAC	2925	0.0	36.076923	5
GTTTTTT	19165	0.0	36.054005	2
CGCGAAA	25	0.0021076733	36.0	42
TAATCGG	100	0.0	36.0	2
ATAGGGA	3480	0.0	35.948277	4
TAACGGG	735	0.0	35.816326	3
AGGGCGA	2300	0.0	35.804348	6
ACGACGG	435	0.0	35.689655	27
TATGGGA	2485	0.0	35.67404	4
CGTAAGG	530	0.0	35.660378	2
>>END_MODULE