FastQCFastQC Report
Thu 26 May 2016
SRR1546840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546840_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4750556
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT601301.265746577874253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG453.8562575E-1045.0000041
CGTCGAA207.035521E-445.09
CGTTTTT375900.043.8507581
GTATCGC501.0822987E-940.59
GCGTAAG3300.038.8636361
TACGGGT13000.038.5961534
GTTTTTT445400.038.018632
CGTCGGA301.1404602E-437.50000411
CGGGTAT7500.037.56
GGGCGAT61750.037.4574937
TAGGGTG44800.037.3158455
TTAGGGT41750.037.239524
TAGGGTA38650.037.0827945
GGCGATA13700.036.9525538
ATAGGGT40300.036.904474
CTAGACG1650.036.818181
GACCGAT14300.036.818189
ACGGGAT11150.036.5246665
ACGGGTA10850.036.4976965
AGGGCGA33200.036.4608426
Produced by FastQC (version 0.11.5)