##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546840_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4750556
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.951114985277513	33.0	31.0	34.0	30.0	34.0
2	32.378915857428055	34.0	31.0	34.0	31.0	34.0
3	32.41239004444953	34.0	31.0	34.0	30.0	34.0
4	36.03328115698457	37.0	35.0	37.0	35.0	37.0
5	36.00077148864259	37.0	35.0	37.0	35.0	37.0
6	36.05301547860924	37.0	35.0	37.0	35.0	37.0
7	36.29400284935068	37.0	36.0	37.0	35.0	37.0
8	36.18005176657216	37.0	37.0	37.0	35.0	37.0
9	38.027278912194696	39.0	39.0	39.0	35.0	39.0
10	37.579879492000515	39.0	37.0	39.0	35.0	39.0
11	37.47621373161373	39.0	37.0	39.0	35.0	39.0
12	37.16371767852016	39.0	37.0	39.0	34.0	39.0
13	37.088633414699245	39.0	37.0	39.0	33.0	39.0
14	38.328781304756745	40.0	37.0	41.0	33.0	41.0
15	38.41673311502906	40.0	37.0	41.0	34.0	41.0
16	38.470292740470796	40.0	37.0	41.0	34.0	41.0
17	38.41401974842524	40.0	37.0	41.0	34.0	41.0
18	38.25378587264312	40.0	37.0	41.0	34.0	41.0
19	38.13668357977466	40.0	37.0	41.0	34.0	41.0
20	37.95449290567252	40.0	35.0	41.0	34.0	41.0
21	37.837784250938206	40.0	35.0	41.0	34.0	41.0
22	37.82631001508034	40.0	35.0	41.0	34.0	41.0
23	37.73273802056012	40.0	35.0	41.0	34.0	41.0
24	37.71372656169088	40.0	35.0	41.0	34.0	41.0
25	37.622073710950886	40.0	35.0	41.0	33.0	41.0
26	37.5997470611861	40.0	35.0	41.0	33.0	41.0
27	37.53754423692722	40.0	35.0	41.0	33.0	41.0
28	37.48146637993532	40.0	35.0	41.0	33.0	41.0
29	37.385501823365516	40.0	35.0	41.0	33.0	41.0
30	37.31136776411014	39.0	35.0	41.0	33.0	41.0
31	37.0908226321298	39.0	35.0	41.0	33.0	41.0
32	36.829096215264066	40.0	35.0	41.0	31.0	41.0
33	36.578018236181194	40.0	35.0	41.0	31.0	41.0
34	36.32078034655312	40.0	35.0	41.0	30.0	41.0
35	36.13820719090565	40.0	35.0	41.0	29.0	41.0
36	36.03689589176509	40.0	35.0	41.0	29.0	41.0
37	35.78011415926893	39.0	35.0	41.0	26.0	41.0
38	35.86926077705431	39.0	35.0	41.0	27.0	41.0
39	35.767553313759485	39.0	35.0	41.0	27.0	41.0
40	35.67857593932163	39.0	35.0	41.0	26.0	41.0
41	35.60608547715257	39.0	35.0	41.0	25.0	41.0
42	35.603410632355455	39.0	35.0	41.0	25.0	41.0
43	35.504441374862225	39.0	35.0	41.0	25.0	41.0
44	35.37822351741565	39.0	35.0	41.0	24.0	41.0
45	35.34944414927432	39.0	35.0	41.0	24.0	41.0
46	35.19101911439419	38.0	35.0	41.0	23.0	41.0
47	35.18529704733509	38.0	35.0	40.0	24.0	41.0
48	35.05288265205168	38.0	35.0	40.0	23.0	41.0
49	35.03447259647081	38.0	35.0	40.0	24.0	41.0
50	34.9347432174255	38.0	35.0	40.0	24.0	41.0
51	34.40529298044271	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	5.0
12	12.0
13	17.0
14	31.0
15	64.0
16	212.0
17	574.0
18	1400.0
19	2849.0
20	5038.0
21	8440.0
22	13123.0
23	19884.0
24	31624.0
25	53048.0
26	79707.0
27	92419.0
28	88925.0
29	82271.0
30	81882.0
31	91970.0
32	111656.0
33	144043.0
34	255084.0
35	484034.0
36	280579.0
37	368398.0
38	657558.0
39	1795026.0
40	677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.347575315394664	23.053512052062956	29.21792733313743	18.380985299404955
2	34.99257771090373	23.832284052645626	28.78886176691739	12.38627646953325
3	27.680675693539875	24.035607621507882	35.24246425050036	13.041252434451883
4	24.455432164150892	25.938795374688773	35.19434356736348	14.411428893796852
5	22.189129019845254	30.57309502298257	33.27905196781177	13.958723989360402
6	19.988607649294103	38.560602169514475	30.956502775675098	10.494287405516323
7	83.6771527374901	3.4806241627295833	11.141222206411207	1.7010008933691132
8	84.92635388362963	2.6390384620242346	10.666730378507273	1.76787727583887
9	80.21435806671893	3.6987459994156473	13.283876666226016	2.803019267639409
10	35.51687423535267	21.459467060276737	30.767640671955032	12.256018032415573
11	32.20058873108748	23.035008954741297	27.92071075469903	16.843691559472195
12	28.680474453937606	22.97592113428407	30.961323264055828	17.382281147722498
13	22.611584833438446	27.54180773787321	31.932599047353616	17.91400838133473
14	18.654426976547587	30.22023106347973	33.26850583384345	17.856836126129235
15	18.361598095044034	24.908200219090144	39.94871337165586	16.78148831420996
16	20.31886372879301	22.658168854340417	38.7576527884315	18.26531462843507
17	20.654719152873895	22.513933105935386	32.670512672621896	24.16083506856882
18	22.055397305073342	24.193252326675026	34.53890449875762	19.21244586949401
19	24.16146657359686	25.299417583962803	31.337973912948296	19.201141929492042
20	25.227426010765896	24.976929016308826	30.63571927159684	19.159925701328433
21	23.191495900690363	25.64261951653659	32.818053297340356	18.347831285432694
22	21.932064373096537	22.774302628997532	33.194956548244036	22.09867644966189
23	19.73206925673542	26.29149093285081	33.061645836824155	20.914793973589617
24	19.831089245132567	25.693855624478484	35.72520774410406	18.74984738628489
25	19.570867073243637	25.988326419054946	34.612243282681014	19.8285632250204
26	19.340746641024754	28.54777419737816	31.953775515960658	20.157703645636428
27	19.339294179460257	27.562605303463428	33.91548273507354	19.18261778200278
28	17.593372228429683	26.952424095200644	37.020003553268296	18.43420012310138
29	18.71551456292695	25.345306949333928	35.82690110378659	20.11227738395253
30	19.608167970233378	25.557113735739563	34.84844721333671	19.986271080690344
31	23.40088191782183	25.24346623847819	31.734474869888913	19.621176973811068
32	23.177750141246626	26.41713938326377	31.28581159763194	19.119298877857666
33	21.901899482923685	26.831743484341626	31.228260439409617	20.038096593325076
34	19.41711664908276	27.65126860940067	31.890098758966317	21.04151598255025
35	19.858349212176428	26.7976632629949	31.810950128784928	21.533037396043746
36	23.218376964717393	27.222287243851035	29.830297758830753	19.729038032600815
37	20.210644817154034	29.085984882611637	30.880406419795914	19.822963880438415
38	20.60381984761363	30.053871588925592	28.299487470519242	21.04282109294154
39	20.238409988220326	28.52501896620101	29.68641986327495	21.550151182303715
40	20.92820293035173	27.35227202878989	29.912456562979155	21.80706847787922
41	19.466058288756095	26.535630776692244	30.287928402485942	23.710382532065722
42	21.641172107012316	27.723365433435582	28.71975406668188	21.915708392870222
43	21.466708317931626	27.821732866637085	29.005362740698143	21.706196074733146
44	20.745150672889658	29.290613561865182	29.564097339343014	20.40013842590215
45	20.09025890864143	29.745149830882955	28.728405685566067	21.436185574909548
46	21.660075157518406	28.37581537824204	29.602029741360802	20.362079722878754
47	21.39614815613162	27.548017537315634	30.060586592390447	20.9952477141623
48	21.299527886841034	26.79793691517372	30.442941836702904	21.45959336128234
49	20.473140407143923	26.16314806098486	31.469853212971284	21.893858318899937
50	19.60524199693678	28.201309488826148	31.017948214903683	21.175500299333383
51	19.899397038999226	29.026686560478392	29.25893305962502	21.81498334089736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3250.0
1	5137.5
2	7025.0
3	55014.5
4	103004.0
5	70871.5
6	38739.0
7	38882.0
8	39025.0
9	39502.5
10	39980.0
11	39352.0
12	38724.0
13	36993.0
14	35262.0
15	33332.5
16	31403.0
17	29725.0
18	28047.0
19	26916.0
20	25785.0
21	26658.5
22	27532.0
23	28839.0
24	30146.0
25	33491.0
26	41646.0
27	46456.0
28	57223.0
29	67990.0
30	79727.0
31	91464.0
32	104289.5
33	117115.0
34	127682.5
35	138250.0
36	153668.5
37	169087.0
38	187087.0
39	205087.0
40	247653.5
41	290220.0
42	324992.0
43	359764.0
44	374786.5
45	389809.0
46	392645.0
47	395481.0
48	385303.0
49	375125.0
50	351754.0
51	328383.0
52	311093.0
53	293803.0
54	268527.5
55	243252.0
56	223705.5
57	204159.0
58	186841.5
59	169524.0
60	150530.5
61	131537.0
62	111580.5
63	91624.0
64	76042.5
65	60461.0
66	49391.5
67	38322.0
68	30863.0
69	23404.0
70	19169.5
71	14935.0
72	12265.5
73	9596.0
74	7518.5
75	4195.0
76	2949.0
77	2137.0
78	1325.0
79	1020.5
80	716.0
81	515.5
82	315.0
83	204.5
84	94.0
85	58.0
86	22.0
87	41.0
88	60.0
89	38.0
90	16.0
91	12.0
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4750556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.859680810569106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73103008977147	16.032924890913115
2	7.056938944276251	2.8029710986600174
3	2.2787699373829278	1.357669307914365
4	1.1858770033964594	0.9420455507219144
5	0.7498564786817776	0.7445955160178711
6	0.5530330852850637	0.6589836330867362
7	0.4168671947873945	0.5795194600212493
8	0.33591889615852183	0.5336993644757562
9	0.2935141263080321	0.5246187175683523
>10	3.582989633090176	17.442682544687223
>50	1.6790191624495359	24.276316016498743
>100	1.1295607142692596	31.388254440652393
>500	0.003909679166424109	0.5720278244346021
>1k	0.0026064527776134367	0.8468047825554504
>5k	0.0	0.0
>10k+	1.0860219906722653E-4	1.2968868517920442
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60130	1.265746577874253	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.210033520286889E-5	0.0	0.0	0.020292361567782802	0.0
2	4.210033520286889E-5	0.0	0.0	0.08784234940078593	0.0
3	6.315050280430332E-5	0.0	0.0	0.14345689220377572	0.0
4	6.315050280430332E-5	0.0	0.0	0.2344988670799797	0.0
5	6.315050280430332E-5	0.0	0.0	0.45796744633680775	0.0
6	6.315050280430332E-5	0.0	0.0	0.7823084287397096	0.0
7	6.315050280430332E-5	0.0	0.0	1.0143444262103214	0.0
8	6.315050280430332E-5	0.0	0.0	1.498035177356082	0.0
9	6.315050280430332E-5	0.0	0.0	1.7548261719259808	0.0
10	6.315050280430332E-5	0.0	0.0	2.1638940789246566	0.0
11	6.315050280430332E-5	0.0	0.0	2.4222848862322643	0.0
12	6.315050280430332E-5	0.0	0.0	2.6651617200176148	0.0
13	6.315050280430332E-5	0.0	0.0	2.764539561263987	0.0
14	6.315050280430332E-5	0.0	0.0	2.8121550403784314	0.0
15	6.315050280430332E-5	0.0	0.0	2.8705903056400137	0.0
16	6.315050280430332E-5	0.0	0.0	2.984303311022962	0.0
17	6.315050280430332E-5	0.0	0.0	3.116077360207942	0.0
18	6.315050280430332E-5	0.0	0.0	3.3086232432582627	0.0
19	6.315050280430332E-5	0.0	0.0	3.4044015058447896	0.0
20	6.315050280430332E-5	0.0	0.0	3.5079472802762455	0.0
21	6.315050280430332E-5	0.0	0.0	3.633995683873635	0.0
22	8.420067040573777E-5	0.0	0.0	3.7743582014399997	0.0
23	8.420067040573777E-5	0.0	0.0	3.926319361354755	0.0
24	8.420067040573777E-5	0.0	0.0	4.0459264136661055	0.0
25	8.420067040573777E-5	0.0	0.0	4.159218415697026	0.0
26	1.0525083800717221E-4	0.0	0.0	4.263774598173351	0.0
27	1.0525083800717221E-4	0.0	0.0	4.377256051712684	0.0
28	1.0525083800717221E-4	0.0	0.0	4.493452976872602	0.0
29	1.0525083800717221E-4	0.0	0.0	4.624195567845111	0.0
30	1.0525083800717221E-4	0.0	0.0	4.791081296589283	0.0
31	1.0525083800717221E-4	0.0	0.0	4.933254128569372	0.0
32	1.0525083800717221E-4	0.0	0.0	5.066838492168075	0.0
33	1.0525083800717221E-4	0.0	0.0	5.201306962806038	0.0
34	1.0525083800717221E-4	0.0	0.0	5.335080777913154	0.0
35	1.0525083800717221E-4	0.0	0.0	5.491757175370631	0.0
36	1.0525083800717221E-4	0.0	0.0	5.630898783216112	0.0
37	1.0525083800717221E-4	0.0	0.0	5.774650377766307	0.0
38	1.0525083800717221E-4	0.0	0.0	5.938673283716685	0.0
39	1.0525083800717221E-4	0.0	0.0	6.142396805763368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	45	3.8562575E-10	45.000004	1
CGTCGAA	20	7.035521E-4	45.0	9
CGTTTTT	37590	0.0	43.850758	1
GTATCGC	50	1.0822987E-9	40.5	9
GCGTAAG	330	0.0	38.863636	1
TACGGGT	1300	0.0	38.596153	4
GTTTTTT	44540	0.0	38.01863	2
CGTCGGA	30	1.1404602E-4	37.500004	11
CGGGTAT	750	0.0	37.5	6
GGGCGAT	6175	0.0	37.457493	7
TAGGGTG	4480	0.0	37.315845	5
TTAGGGT	4175	0.0	37.23952	4
TAGGGTA	3865	0.0	37.082794	5
GGCGATA	1370	0.0	36.952553	8
ATAGGGT	4030	0.0	36.90447	4
CTAGACG	165	0.0	36.81818	1
GACCGAT	1430	0.0	36.81818	9
ACGGGAT	1115	0.0	36.524666	5
ACGGGTA	1085	0.0	36.497696	5
AGGGCGA	3320	0.0	36.460842	6
>>END_MODULE