FastQCFastQC Report
Thu 26 May 2016
SRR1546839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546839_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3476261
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335390.964800974380232No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44820.1289316308528042No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA207.035261E-445.015
CTATGCG1550.043.5483861
CGTTTTT190300.042.8836061
AGTACGG2900.042.6724132
CGGTCTA4250.039.1764731
CTCACGA4250.038.6470624
TAGGGAC23650.038.150115
GCGATAT3600.038.1259
CGCATAT301.1403972E-437.50000412
TCGAACG301.1403972E-437.5000041
GGGCGAT51550.037.2308467
TACGGGA8450.037.0118374
GGCGATA12850.036.7704288
ACGGGTA3650.036.3698655
CGACGGT4900.036.2755128
GTACGGG10650.036.126763
GTAGGGA37700.035.629974
GTTTTTT242950.035.5628742
CGTAAGG4250.035.470592
ACGGGAT7300.035.4452065
Produced by FastQC (version 0.11.5)