##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546839_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3476261
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937721304585587	33.0	31.0	34.0	30.0	34.0
2	32.33400484025797	34.0	31.0	34.0	31.0	34.0
3	32.384046537357236	34.0	31.0	34.0	30.0	34.0
4	35.99998590439556	37.0	35.0	37.0	35.0	37.0
5	35.96196689489081	37.0	35.0	37.0	35.0	37.0
6	36.01196745583833	37.0	35.0	37.0	35.0	37.0
7	36.244993111852075	37.0	35.0	37.0	35.0	37.0
8	36.11478597262979	37.0	36.0	37.0	35.0	37.0
9	37.90924473162401	39.0	39.0	39.0	35.0	39.0
10	37.60321017322922	39.0	37.0	39.0	35.0	39.0
11	37.450172757454055	39.0	37.0	39.0	35.0	39.0
12	36.995026265289056	39.0	35.0	39.0	34.0	39.0
13	36.87868200920472	39.0	35.0	39.0	33.0	39.0
14	38.07783880439357	40.0	36.0	41.0	33.0	41.0
15	38.188128279205735	40.0	36.0	41.0	33.0	41.0
16	38.24707408333264	40.0	36.0	41.0	34.0	41.0
17	38.18742407431433	40.0	36.0	41.0	34.0	41.0
18	38.07909618984305	40.0	36.0	41.0	34.0	41.0
19	38.001091977846315	40.0	36.0	41.0	34.0	41.0
20	37.8597573657444	40.0	35.0	41.0	34.0	41.0
21	37.734055641967046	40.0	35.0	41.0	33.0	41.0
22	37.68824320153176	40.0	35.0	41.0	33.0	41.0
23	37.58891895631542	40.0	35.0	41.0	33.0	41.0
24	37.55552877071083	39.0	35.0	41.0	33.0	41.0
25	37.4541891417244	39.0	35.0	41.0	33.0	41.0
26	37.41923348103034	39.0	35.0	41.0	33.0	41.0
27	37.35959900594345	39.0	35.0	41.0	33.0	41.0
28	37.30019782749339	39.0	35.0	41.0	33.0	41.0
29	37.19091949655103	39.0	35.0	41.0	33.0	41.0
30	37.13990549041053	39.0	35.0	41.0	33.0	41.0
31	36.955807403414184	39.0	35.0	41.0	32.0	41.0
32	36.77343128148318	39.0	35.0	41.0	31.0	41.0
33	36.626093380215124	39.0	35.0	41.0	31.0	41.0
34	36.43636453074151	39.0	35.0	41.0	31.0	41.0
35	36.29381712132662	39.0	35.0	41.0	30.0	41.0
36	36.19403750178712	39.0	35.0	41.0	30.0	41.0
37	35.927230722894514	39.0	35.0	41.0	29.0	41.0
38	36.00250642860246	39.0	35.0	41.0	30.0	41.0
39	35.89869086354563	39.0	35.0	41.0	29.0	41.0
40	35.78215415931082	38.0	35.0	41.0	29.0	41.0
41	35.705886007983864	38.0	35.0	41.0	28.0	41.0
42	35.69971443456058	38.0	35.0	41.0	29.0	41.0
43	35.58308682806038	38.0	35.0	41.0	28.0	41.0
44	35.445949541763405	38.0	35.0	40.0	27.0	41.0
45	35.39355071440263	38.0	35.0	40.0	27.0	41.0
46	35.22511888491687	38.0	35.0	40.0	27.0	41.0
47	35.19600542076674	37.0	35.0	40.0	27.0	41.0
48	35.06356801172294	37.0	35.0	40.0	26.0	41.0
49	35.02209414080243	37.0	35.0	40.0	26.0	41.0
50	34.90454456670543	37.0	34.0	40.0	26.0	41.0
51	34.35944654328314	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	5.0
12	7.0
13	17.0
14	33.0
15	88.0
16	185.0
17	479.0
18	1012.0
19	2190.0
20	3871.0
21	6330.0
22	9606.0
23	14218.0
24	21066.0
25	32703.0
26	46938.0
27	55217.0
28	56511.0
29	56688.0
30	61760.0
31	72030.0
32	87952.0
33	116846.0
34	215107.0
35	417741.0
36	223496.0
37	299233.0
38	499367.0
39	1175075.0
40	484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.91023832790461	22.266423608584052	26.919612767856037	15.903725295655303
2	34.9169696981901	23.847403862943548	27.86896611042727	13.366660328439089
3	29.75910036674461	24.28416623492885	32.21029721301134	13.7464361853152
4	26.12729596540651	26.67342872126115	31.905112993529546	15.294162319802801
5	23.806123878500493	30.486404789513795	30.337106448566438	15.370364883419283
6	20.759833625841097	40.008762287987004	28.502520380374204	10.728883705797694
7	83.86565335571754	4.483035077055492	9.265529832196144	2.3857817350308275
8	84.1794675371038	3.8740761985363013	9.139215956454363	2.807240307905534
9	78.88334621594869	5.582118258669301	11.495138023295718	4.039397502086294
10	40.794721685166905	25.910568855445547	20.549118722673583	12.745590736713957
11	36.20596957478164	23.718472232090743	24.091746850998817	15.983811342128798
12	31.09231441482673	22.493305307052605	29.272312982253055	17.142067295867598
13	22.794001946344075	29.664832416208103	30.40050790202462	17.140657735423204
14	18.45807895322014	32.622838158584756	31.21028599406086	17.70879689413424
15	17.784913158131683	23.99776656585912	40.875728260910215	17.341592015098982
16	20.15749105144867	21.47146028448382	39.002278597608175	19.368770066459337
17	20.42910471912207	21.56650493159173	30.669503814586996	27.334886534699205
18	23.179818776553315	23.57619292682569	32.293087314214894	20.950900982406097
19	26.20180130318178	24.886134844305417	28.64068607046479	20.27137778204801
20	27.459273052282324	24.323892826229102	27.160446238070158	21.05638788341842
21	25.036123582205132	25.41670490219233	29.133514428289477	20.413657087313062
22	23.705124557678495	23.408627833180535	29.249443583206208	23.63680402593476
23	21.67518491850871	27.313656828414207	28.43722033529703	22.57393791778005
24	21.292676240362848	25.080855551409975	33.51733370998323	20.109134498243947
25	21.023162530086204	25.49696354790391	32.43643098144817	21.043442940561714
26	20.033449732341733	28.986373577818235	28.942504604803837	22.037672085036192
27	20.771196409015317	27.189557976227906	31.202288896029383	20.836956718727393
28	19.363505789697612	25.974430573538637	34.736287062450145	19.92577657431361
29	19.749696584922706	25.45450413533391	34.02365357491857	20.77214570482481
30	21.52562767870422	25.7793646679579	31.711082683377338	20.98392496996054
31	26.026411710743236	24.897986658654226	27.836632519825177	21.238969110777354
32	25.691454122691017	26.166677358230583	28.35385490329984	19.788013615778564
33	24.56219484095124	25.611598208534975	28.403218285393418	21.422988665120368
34	20.826802130219797	27.55846008110438	30.064054453908955	21.550683334766866
35	21.740600029744602	26.32132627555871	30.254604012759685	21.683469681937
36	25.45899171552424	25.921011109349962	28.297040987428733	20.32295618769707
37	22.23604614268031	28.752472843667377	28.114172094672984	20.89730891897933
38	22.30796249188424	29.99219563778439	26.246964770481846	21.45287709984952
39	22.267372904393543	27.541459056152572	27.115973167722444	23.075194871731437
40	22.954519237767247	25.788771326433775	27.871526332458927	23.385183103340054
41	20.186660322685782	25.417999396477995	28.429741034979823	25.965599245856396
42	22.808011251169	27.06275506931154	26.678376566086374	23.45085711343308
43	22.26553184585392	27.040230868740867	27.357525801428608	23.336711483976604
44	21.627748894573795	28.558442533515176	28.544375695610885	21.26943287630014
45	21.08219146951279	29.538921272021867	27.011234196741846	22.3676530617235
46	23.00839896659083	27.625284752784673	28.52504458094487	20.841271699679627
47	22.634894215365303	26.87209044430208	28.50401624043764	21.988999099894972
48	23.388261123085982	25.376575579336535	28.80508684474497	22.430076452832513
49	21.542916369052843	25.068284573569134	29.95413750578567	23.434661551592356
50	20.50309225918307	27.982478876010745	29.16357546225672	22.350853402549465
51	20.95556116183451	29.044855952990872	27.348608174127314	22.6509747110473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2485.0
1	4298.5
2	6112.0
3	30826.5
4	55541.0
5	37513.5
6	19486.0
7	19632.5
8	19779.0
9	20236.0
10	20693.0
11	20477.0
12	20261.0
13	19561.0
14	18861.0
15	18295.0
16	17729.0
17	16905.0
18	16081.0
19	15445.5
20	14810.0
21	15195.0
22	15580.0
23	17447.5
24	19315.0
25	20820.5
26	25515.5
27	28705.0
28	33749.5
29	38794.0
30	46374.5
31	53955.0
32	62385.0
33	70815.0
34	77213.5
35	83612.0
36	91796.5
37	99981.0
38	112203.0
39	124425.0
40	158385.5
41	192346.0
42	221739.5
43	251133.0
44	267624.5
45	284116.0
46	280185.0
47	276254.0
48	272648.5
49	269043.0
50	258912.0
51	248781.0
52	236323.5
53	223866.0
54	206663.5
55	189461.0
56	179400.0
57	169339.0
58	161177.0
59	153015.0
60	140025.0
61	127035.0
62	113648.0
63	100261.0
64	88398.0
65	76535.0
66	63069.0
67	49603.0
68	42799.5
69	35996.0
70	30345.5
71	24695.0
72	19866.0
73	15037.0
74	12564.0
75	7952.0
76	5813.0
77	4013.5
78	2214.0
79	1697.5
80	1181.0
81	918.0
82	655.0
83	444.0
84	233.0
85	183.0
86	133.0
87	84.5
88	36.0
89	31.5
90	27.0
91	17.5
92	8.0
93	5.0
94	2.0
95	2.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3476261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.96117377927913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1268295142431	16.193867420957574
2	6.725773934383106	2.685086846087341
3	2.1716439106813574	1.3004568446347171
4	1.1299666796412742	0.9022184502845799
5	0.7575155363474773	0.7560449630767914
6	0.5143176471561821	0.6159830359580709
7	0.4118853856328354	0.5755201031833699
8	0.30248724543755756	0.4830400377755634
9	0.27862435223789517	0.5005502202743733
>10	3.679482596084542	18.204752974309944
>50	1.8472734984091268	26.960305563716346
>100	1.047395126080173	28.45916277217494
>500	0.004585690948421016	0.641813413520721
>1k	0.002070957202512717	0.7308688632436918
>5k	0.0	0.0
>10k+	1.4792551446519408E-4	0.9903284908019626
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33539	0.964800974380232	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4482	0.1289316308528042	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4383269840785833E-4	0.0	0.0	0.028219975427621804	0.0
2	1.4383269840785833E-4	0.0	0.0	0.09334742126670005	0.0
3	1.7259923808942999E-4	0.0	0.0	0.1512832321853854	0.0
4	1.7259923808942999E-4	0.0	0.0	0.2368349211983795	0.0
5	1.7259923808942999E-4	0.0	0.0	0.4431773103342931	0.0
6	1.7259923808942999E-4	0.0	0.0	0.793352397878065	0.0
7	1.7259923808942999E-4	0.0	0.0	1.0349625646635854	0.0
8	1.7259923808942999E-4	0.0	0.0	1.652982903182471	0.0
9	1.7259923808942999E-4	0.0	0.0	1.9783900000604098	0.0
10	2.0136577777100166E-4	0.0	0.0	2.4590501115997907	0.0
11	2.0136577777100166E-4	0.0	0.0	2.744241586002892	0.0
12	2.0136577777100166E-4	0.0	0.0	3.020141468088846	0.0
13	2.0136577777100166E-4	0.0	0.0	3.135926790307172	0.0
14	2.0136577777100166E-4	0.0	0.0	3.182327218813547	0.0
15	2.3013231745257331E-4	0.0	0.0	3.238076772716433	0.0
16	2.3013231745257331E-4	0.0	0.0	3.351186806744373	0.0
17	2.3013231745257331E-4	0.0	0.0	3.474192530422773	0.0
18	2.3013231745257331E-4	0.0	0.0	3.6791541256539713	0.0
19	2.3013231745257331E-4	0.0	0.0	3.7727029126984424	0.0
20	2.3013231745257331E-4	0.0	0.0	3.8766651871076423	0.0
21	2.3013231745257331E-4	0.0	0.0	3.999124346532093	0.0
22	2.3013231745257331E-4	0.0	0.0	4.1301271682419705	0.0
23	2.3013231745257331E-4	0.0	0.0	4.277613217189388	0.0
24	2.3013231745257331E-4	0.0	0.0	4.393168407090261	0.0
25	2.3013231745257331E-4	0.0	0.0	4.48996781311875	0.0
26	2.3013231745257331E-4	0.0	0.0	4.586249421432971	0.0
27	2.3013231745257331E-4	0.0	0.0	4.686213146826432	0.0
28	2.3013231745257331E-4	0.0	0.0	4.792937009045063	0.0
29	2.3013231745257331E-4	0.0	0.0	4.909815459771289	0.0
30	2.3013231745257331E-4	0.0	0.0	5.082184565543266	0.0
31	2.58898857134145E-4	0.0	0.0	5.214280515761043	0.0
32	2.58898857134145E-4	0.0	0.0	5.351094178486598	0.0
33	2.58898857134145E-4	0.0	0.0	5.474819065657038	0.0
34	2.58898857134145E-4	0.0	0.0	5.601478139874998	0.0
35	2.58898857134145E-4	0.0	0.0	5.748590223806556	0.0
36	2.58898857134145E-4	0.0	0.0	5.880772473643377	0.0
37	2.58898857134145E-4	0.0	0.0	6.018966930273647	0.0
38	2.58898857134145E-4	0.0	0.0	6.163662624871953	0.0
39	2.58898857134145E-4	0.0	0.0	6.335456399850299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGA	20	7.035261E-4	45.0	15
CTATGCG	155	0.0	43.548386	1
CGTTTTT	19030	0.0	42.883606	1
AGTACGG	290	0.0	42.672413	2
CGGTCTA	425	0.0	39.17647	31
CTCACGA	425	0.0	38.64706	24
TAGGGAC	2365	0.0	38.15011	5
GCGATAT	360	0.0	38.125	9
CGCATAT	30	1.1403972E-4	37.500004	12
TCGAACG	30	1.1403972E-4	37.500004	1
GGGCGAT	5155	0.0	37.230846	7
TACGGGA	845	0.0	37.011837	4
GGCGATA	1285	0.0	36.770428	8
ACGGGTA	365	0.0	36.369865	5
CGACGGT	490	0.0	36.27551	28
GTACGGG	1065	0.0	36.12676	3
GTAGGGA	3770	0.0	35.62997	4
GTTTTTT	24295	0.0	35.562874	2
CGTAAGG	425	0.0	35.47059	2
ACGGGAT	730	0.0	35.445206	5
>>END_MODULE