FastQCFastQC Report
Thu 26 May 2016
SRR1546838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546838_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2130262
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223331.0483686983103486No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24710.11599512172681106No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.0346444E-445.028
CGTTTTT151600.043.6494061
CGGTCTA1900.040.2631631
TATAGCG900.040.0000041
TATCGCG403.4600816E-739.3751
TACGGGT5450.038.807344
CTATACG659.094947E-1238.076921
ATAGCGG3550.038.028172
ACGGGTA4050.037.777785
ACGGGTG8000.037.406255
TTTAGCG1150.037.1739161
CGAGGGT7650.037.0588234
GTTTTTT184100.037.055952
TCACGAC1950.036.92307725
CGACGGT1950.036.92307728
TAGGGTA19600.036.3903055
ACGGGTC4900.036.275515
ACGGTCT2050.036.21951330
ATAGGGT20250.036.111114
TTACGGG5800.036.0775873
Produced by FastQC (version 0.11.5)