##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546838_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2130262
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.914566846707118	33.0	31.0	34.0	30.0	34.0
2	32.3264682935714	33.0	31.0	34.0	31.0	34.0
3	32.37513413842992	34.0	31.0	34.0	30.0	34.0
4	36.01147886973527	37.0	35.0	37.0	35.0	37.0
5	35.976118430502915	37.0	35.0	37.0	35.0	37.0
6	36.02177478638778	37.0	35.0	37.0	35.0	37.0
7	36.26624142945797	37.0	36.0	37.0	35.0	37.0
8	36.1439926168706	37.0	36.0	37.0	35.0	37.0
9	37.97120025611873	39.0	39.0	39.0	35.0	39.0
10	37.54467056164923	39.0	37.0	39.0	35.0	39.0
11	37.457449834809054	39.0	37.0	39.0	35.0	39.0
12	37.145155854068655	39.0	37.0	39.0	34.0	39.0
13	37.07890250119469	39.0	37.0	39.0	33.0	39.0
14	38.30040060800033	40.0	37.0	41.0	33.0	41.0
15	38.37901769829251	40.0	37.0	41.0	34.0	41.0
16	38.423487345687995	40.0	37.0	41.0	34.0	41.0
17	38.36373272395602	40.0	37.0	41.0	34.0	41.0
18	38.212062178267274	40.0	37.0	41.0	34.0	41.0
19	38.1071750798728	40.0	37.0	41.0	34.0	41.0
20	37.96311392683153	40.0	35.0	41.0	34.0	41.0
21	37.85311572003819	40.0	35.0	41.0	34.0	41.0
22	37.830292705779854	40.0	35.0	41.0	34.0	41.0
23	37.72376965838005	40.0	35.0	41.0	33.0	41.0
24	37.71645084031917	40.0	35.0	41.0	33.0	41.0
25	37.62684120544797	40.0	35.0	41.0	33.0	41.0
26	37.58655695872151	40.0	35.0	41.0	33.0	41.0
27	37.537504776407786	40.0	35.0	41.0	33.0	41.0
28	37.47088480196333	40.0	35.0	41.0	33.0	41.0
29	37.37597628836265	39.0	35.0	41.0	33.0	41.0
30	37.294419184119135	39.0	35.0	41.0	33.0	41.0
31	37.10436697457872	39.0	35.0	41.0	33.0	41.0
32	36.86994369706637	39.0	35.0	41.0	31.0	41.0
33	36.6544373415101	40.0	35.0	41.0	31.0	41.0
34	36.40164026772294	40.0	35.0	41.0	30.0	41.0
35	36.22897230481509	40.0	35.0	41.0	30.0	41.0
36	36.12435700397416	39.0	35.0	41.0	29.0	41.0
37	35.869233455790884	39.0	35.0	41.0	27.0	41.0
38	35.95602231087068	39.0	35.0	41.0	28.0	41.0
39	35.85782499992958	39.0	35.0	41.0	27.0	41.0
40	35.76652167667639	39.0	35.0	41.0	27.0	41.0
41	35.70213757744352	39.0	35.0	41.0	27.0	41.0
42	35.69605147160302	39.0	35.0	41.0	27.0	41.0
43	35.60482701188868	39.0	35.0	41.0	26.0	41.0
44	35.47804401524319	39.0	35.0	41.0	26.0	41.0
45	35.430553143228394	39.0	35.0	41.0	26.0	41.0
46	35.267031003698136	38.0	35.0	40.0	24.0	41.0
47	35.25508881067211	38.0	35.0	40.0	24.0	41.0
48	35.119255284091814	38.0	35.0	40.0	24.0	41.0
49	35.09404242295079	38.0	35.0	40.0	24.0	41.0
50	34.98304903340528	38.0	35.0	40.0	24.0	41.0
51	34.450886322903	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	2.0
14	18.0
15	50.0
16	91.0
17	270.0
18	624.0
19	1255.0
20	2234.0
21	3806.0
22	5811.0
23	8632.0
24	13533.0
25	22668.0
26	33274.0
27	38646.0
28	37774.0
29	36240.0
30	37719.0
31	42389.0
32	51486.0
33	67225.0
34	117702.0
35	214178.0
36	129089.0
37	171571.0
38	305904.0
39	787813.0
40	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.21641281682723	22.262519821505524	27.51614590130228	18.00492146036497
2	34.28747262073867	24.010567714206047	28.961038595252603	12.740921069802683
3	27.92252783929864	24.584346901930374	34.64193606232473	12.851189196446258
4	25.237599882080232	26.223957428710648	34.29657948177267	14.241863207436456
5	22.807664033813683	30.77879622318757	32.69452302111196	13.719016721886792
6	20.146864564077095	38.98140228760594	30.150469754424574	10.721263393892395
7	84.2724040517082	3.779112616194628	10.168561425777673	1.7799219063195044
8	84.80919248430475	3.0773679481678777	10.065522456862114	2.047917110665261
9	79.51838787904963	4.39917719041132	12.80781425007816	3.274620680460901
10	35.342694936115834	22.77743301058743	28.800541905174104	13.079330148122626
11	32.123842043842494	23.676195697993958	27.45225704631637	16.74770521184718
12	29.285646554273605	23.449040540553227	29.89078338720777	17.374529517965396
13	23.41068845052862	27.807236856311572	31.025338667262524	17.756736025897286
14	18.932976319344757	30.523475516157166	32.79399435374616	17.749553810751916
15	18.262072928118698	25.333832176511624	39.72793017948027	16.676164715889406
16	20.907146632667718	22.941309566616688	38.21689538657686	17.934648414138728
17	21.379389014121266	22.8278024017703	32.01254118038063	23.7802674037278
18	22.561966556226416	24.208524585238813	33.658301185487986	19.57120767304679
19	24.504966994670138	25.88662802979164	30.47873923489223	19.129665740645986
20	25.515499971365024	25.77748652513165	29.739816041407114	18.967197462096212
21	23.519970782936557	26.267332375078745	31.79852994608175	18.414166895902945
22	22.42940070282435	23.578367355752487	32.34142091442273	21.650811027000437
23	20.07889170440068	27.053151208630677	32.03746769176749	20.830489395201155
24	20.307032656077045	25.616802064722556	35.2382476897208	18.837917589479606
25	20.129777463992692	26.71812199626149	33.07372520375428	20.078375335991534
26	19.449391671071446	29.088112166484688	31.051485685798276	20.411010476645597
27	20.015894758485107	27.389635641061993	32.380946569013574	20.213523031439326
28	18.341499777961584	27.20416549701398	35.414798743065404	19.039535981959027
29	19.27331004355333	25.639897815386085	35.06033530147935	20.026456839581236
30	20.60474251523991	26.095851120660274	32.97815010547998	20.321256258619833
31	23.73970901231867	25.20117243794425	30.503008550122	20.55610999961507
32	24.047934009995014	26.53743999564373	30.032503044226484	19.38212295013477
33	23.313329534113645	26.345585660355393	29.764836437959275	20.57624836757169
34	20.14371941103958	27.153796105831113	32.161959420953856	20.54052506217545
35	20.939020646286703	27.229890032305885	31.03097177718046	20.800117544226953
36	23.097487539091436	27.6620434481768	29.79211007847861	19.448358934253157
37	21.30127655659257	29.128107246902026	29.642973493401282	19.927642703104127
38	20.976762482736866	30.20731722201307	28.288773869129713	20.527146426120353
39	21.090410475331204	28.75190938954927	28.5714151592621	21.586264975857432
40	21.909042174155104	27.450003802349194	29.236309899909024	21.404644123586678
41	19.675701862024482	26.83698061552992	29.384366805585415	24.10295071686018
42	21.594573812986383	27.8132455068907	28.2808875152446	22.31129316487831
43	21.37488252618692	28.120015284504913	28.590943273644275	21.914158915663894
44	21.161387660297184	28.566861728745103	29.267432832205614	21.004317778752096
45	20.738716646121464	29.644522598628715	27.963180115873072	21.653580639376752
46	21.98818736850209	28.97047405436514	28.679007558694657	20.362331018438105
47	21.32296402977662	27.7267772696504	29.69526753047278	21.254991170100205
48	21.637714046441236	26.555982315790267	29.994948978106915	21.811354659661582
49	21.30470336512598	25.877333398427048	30.682892526834728	22.135070709612243
50	19.933698296265906	27.967921316720666	30.338944223762148	21.75943616325128
51	19.790007050775916	28.875086726421443	29.208050465154052	22.12685575764859
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1508.0
1	2236.0
2	2964.0
3	21481.5
4	39999.0
5	27680.0
6	15361.0
7	15395.0
8	15429.0
9	15726.5
10	16024.0
11	15604.0
12	15184.0
13	14590.5
14	13997.0
15	13330.5
16	12664.0
17	12165.5
18	11667.0
19	11374.0
20	11081.0
21	11222.5
22	11364.0
23	12457.0
24	13550.0
25	15231.0
26	19152.5
27	21393.0
28	25957.5
29	30522.0
30	36612.5
31	42703.0
32	48342.0
33	53981.0
34	58654.0
35	63327.0
36	71651.5
37	79976.0
38	87377.0
39	94778.0
40	110717.0
41	126656.0
42	139076.5
43	151497.0
44	156778.0
45	162059.0
46	166318.5
47	170578.0
48	171274.0
49	171970.0
50	163980.5
51	155991.0
52	146557.0
53	137123.0
54	122146.0
55	107169.0
56	100186.5
57	93204.0
58	85104.0
59	77004.0
60	69463.5
61	61923.0
62	54369.5
63	46816.0
64	39535.5
65	32255.0
66	25617.5
67	18980.0
68	16252.5
69	13525.0
70	10397.0
71	7269.0
72	6381.0
73	5493.0
74	4417.0
75	2460.0
76	1579.0
77	1237.0
78	895.0
79	608.5
80	322.0
81	221.0
82	120.0
83	80.0
84	40.0
85	30.0
86	20.0
87	23.0
88	26.0
89	21.0
90	16.0
91	10.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2130262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.311979539550272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73003377779162	18.353529365759798
2	5.788626788950592	2.6988869853221757
3	2.2830790958206655	1.5966927950683885
4	1.3043573780560498	1.2162861003801628
5	0.9199592926784042	1.0723036104069046
6	0.70619910269413	0.9877739419712591
7	0.5913006317408159	0.9649071760205534
8	0.48449321820550845	0.9035596791886141
9	0.4452794559303509	0.9342311009427376
>10	6.9847761258998275	40.49179265564271
>50	1.6407049129942735	25.388731182423264
>100	0.11769830844122434	3.695817215942867
>500	0.0028756912442491076	0.4556672397451136
>1k	4.108130348927296E-4	0.1704203138138776
>5k	0.0	0.0
>10k+	2.054065174463648E-4	1.0694006373715448
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22333	1.0483686983103486	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2471	0.11599512172681106	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028353319920272717	0.0
2	0.0	0.0	0.0	0.10177151918402526	0.0
3	0.0	0.0	0.0	0.17242010607145974	0.0
4	0.0	0.0	0.0	0.26316011833286235	0.0
5	0.0	0.0	0.0	0.4885314576329109	0.0
6	0.0	0.0	0.0	0.8833185777148539	0.0
7	0.0	0.0	0.0	1.167368145326725	0.0
8	0.0	0.0	0.0	1.7970559489865565	0.0
9	0.0	0.0	0.0	2.134526175653511	0.0
10	0.0	0.0	0.0	2.5659754527846808	0.0
11	0.0	0.0	0.0	2.8257557051667823	0.0
12	0.0	0.0	0.0	3.0719695511631904	0.0
13	0.0	0.0	0.0	3.193034471816143	0.0
14	0.0	0.0	0.0	3.2473939825242155	0.0
15	0.0	0.0	0.0	3.305555842426894	0.0
16	4.694258264945814E-5	0.0	0.0	3.408219270681259	0.0
17	4.694258264945814E-5	0.0	0.0	3.5182526844115887	0.0
18	4.694258264945814E-5	0.0	0.0	3.6909544459789454	0.0
19	4.694258264945814E-5	0.0	0.0	3.772493712041054	0.0
20	4.694258264945814E-5	0.0	0.0	3.870603709778422	0.0
21	4.694258264945814E-5	0.0	0.0	3.9798390996037107	0.0
22	4.694258264945814E-5	0.0	0.0	4.10381446038093	0.0
23	4.694258264945814E-5	0.0	0.0	4.23717833768804	0.0
24	4.694258264945814E-5	0.0	0.0	4.348854741811101	0.0
25	4.694258264945814E-5	0.0	0.0	4.450673203577776	0.0
26	4.694258264945814E-5	0.0	0.0	4.550238421377276	0.0
27	4.694258264945814E-5	0.0	0.0	4.648113706201396	0.0
28	4.694258264945814E-5	0.0	0.0	4.752514010013792	0.0
29	4.694258264945814E-5	0.0	0.0	4.869494925976241	0.0
30	4.694258264945814E-5	0.0	0.0	5.031259065786275	0.0
31	4.694258264945814E-5	0.0	0.0	5.154342517493154	0.0
32	4.694258264945814E-5	0.0	0.0	5.283575447527111	0.0
33	4.694258264945814E-5	0.0	0.0	5.401823813221097	0.0
34	4.694258264945814E-5	0.0	0.0	5.526597197903357	0.0
35	4.694258264945814E-5	0.0	0.0	5.65972636229722	0.0
36	4.694258264945814E-5	0.0	0.0	5.7881143258434875	0.0
37	4.694258264945814E-5	0.0	0.0	5.91091612205447	0.0
38	4.694258264945814E-5	0.0	0.0	6.060616018123592	0.0
39	4.694258264945814E-5	0.0	0.0	6.263971286161045	4.694258264945814E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0346444E-4	45.0	28
CGTTTTT	15160	0.0	43.649406	1
CGGTCTA	190	0.0	40.26316	31
TATAGCG	90	0.0	40.000004	1
TATCGCG	40	3.4600816E-7	39.375	1
TACGGGT	545	0.0	38.80734	4
CTATACG	65	9.094947E-12	38.07692	1
ATAGCGG	355	0.0	38.02817	2
ACGGGTA	405	0.0	37.77778	5
ACGGGTG	800	0.0	37.40625	5
TTTAGCG	115	0.0	37.173916	1
CGAGGGT	765	0.0	37.058823	4
GTTTTTT	18410	0.0	37.05595	2
TCACGAC	195	0.0	36.923077	25
CGACGGT	195	0.0	36.923077	28
TAGGGTA	1960	0.0	36.390305	5
ACGGGTC	490	0.0	36.27551	5
ACGGTCT	205	0.0	36.219513	30
ATAGGGT	2025	0.0	36.11111	4
TTACGGG	580	0.0	36.077587	3
>>END_MODULE