FastQCFastQC Report
Thu 26 May 2016
SRR1546837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546837_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2369456
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542032.287571493203503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT322000.043.93091
AGGGCGA17300.038.8872836
GTTTTTT372800.038.8559532
GGGCGAT32450.038.274277
TACGGGT3800.037.8947374
TAGTACG950.037.8947371
ATTAGCG1150.037.1739161
GTACGAG2950.036.610171
TATACGG1500.036.02
GGCGATA6100.035.778698
TAGGGAC14850.035.7575765
CGAATAT1450.035.68965514
TGGGCGA10350.035.6521726
CTATGCG950.035.5263141
TAGGGTA16200.035.4166645
GTAGGGT15050.035.282394
TATGGGC8050.035.2173924
TAGGGTC12100.035.144635
TCGTTAG900.035.01
TAGGGCG7700.034.7727285