FastQCFastQC Report
Thu 26 May 2016
SRR1546836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546836_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1920713
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC95590.49767976787786616No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.312852570894246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19960.10391974230402981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC302.1662654E-645.00000436
GTTTACG600.045.0000041
TTCACCG302.1662654E-645.00000415
ACGATTC302.1662654E-645.00000426
GTCACAA302.1662654E-645.00000421
GATATCG302.1662654E-645.00000411
CCGACAC302.1662654E-645.00000445
CACGTTA302.1662654E-645.00000412
ACGAGTT302.1662654E-645.00000429
TAGTCGA302.1662654E-645.00000431
TCACGGT207.034471E-445.022
TCTGCGT253.8917868E-545.045
TCGCCTC207.034471E-445.036
CTATGCG1350.045.01
CACGGTT207.034471E-445.016
ACGCATC253.8917868E-545.018
CGACGAT207.034471E-445.038
TCCGATT207.034471E-445.037
CCCTAGT207.034471E-445.015
CGTTGAT502.1827873E-1145.025
Produced by FastQC (version 0.11.5)