##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546836_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1920713
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09684164161954	33.0	31.0	34.0	31.0	34.0
2	32.47352363419209	34.0	31.0	34.0	31.0	34.0
3	32.509103650571426	34.0	31.0	34.0	31.0	34.0
4	36.06842719344327	37.0	35.0	37.0	35.0	37.0
5	36.01088710286232	37.0	35.0	37.0	35.0	37.0
6	36.072964050329226	37.0	35.0	37.0	35.0	37.0
7	36.31694948698738	37.0	37.0	37.0	35.0	37.0
8	36.22989275336815	37.0	37.0	37.0	35.0	37.0
9	38.09594874403412	39.0	39.0	39.0	37.0	39.0
10	37.82502487357559	39.0	38.0	39.0	35.0	39.0
11	37.48291493835883	39.0	37.0	39.0	35.0	39.0
12	36.16252193846764	37.0	35.0	39.0	33.0	39.0
13	35.67617858576477	37.0	35.0	39.0	32.0	39.0
14	36.58146323787052	38.0	35.0	41.0	33.0	41.0
15	36.90187550144139	38.0	35.0	41.0	33.0	41.0
16	37.09186380266078	38.0	35.0	41.0	34.0	41.0
17	37.06841730128343	37.0	35.0	41.0	34.0	41.0
18	37.058683936642275	37.0	35.0	41.0	34.0	41.0
19	37.001802455650584	37.0	35.0	41.0	34.0	41.0
20	36.839021238467176	36.0	35.0	41.0	34.0	41.0
21	36.64245360967516	36.0	35.0	41.0	33.0	41.0
22	36.586263017952184	35.0	35.0	41.0	33.0	41.0
23	36.58562679588257	35.0	35.0	41.0	33.0	41.0
24	36.53896808112404	35.0	35.0	41.0	33.0	41.0
25	36.41795260405901	35.0	35.0	40.0	33.0	41.0
26	36.349858620210306	35.0	35.0	40.0	33.0	41.0
27	36.27354685473571	35.0	35.0	40.0	33.0	41.0
28	36.31073200420885	36.0	35.0	40.0	33.0	41.0
29	36.324226472148624	36.0	35.0	40.0	33.0	41.0
30	36.34112384307286	36.0	35.0	40.0	33.0	41.0
31	36.115350393317485	36.0	35.0	40.0	33.0	41.0
32	35.89969922627691	35.0	35.0	40.0	32.0	41.0
33	35.80657547483669	35.0	35.0	40.0	32.0	41.0
34	35.726943067496286	35.0	35.0	40.0	31.0	41.0
35	35.64353393765752	35.0	35.0	40.0	31.0	41.0
36	35.489137627537275	35.0	35.0	40.0	31.0	41.0
37	35.199770085379754	35.0	35.0	40.0	30.0	41.0
38	35.31345495136441	35.0	35.0	40.0	30.0	41.0
39	35.303571642405714	35.0	35.0	40.0	30.0	41.0
40	35.15447805059892	35.0	35.0	40.0	30.0	41.0
41	35.174107740198565	35.0	35.0	40.0	30.0	41.0
42	35.168646747327685	35.0	35.0	40.0	30.0	41.0
43	35.01245162603679	35.0	35.0	40.0	30.0	41.0
44	34.90458178811723	35.0	34.0	40.0	30.0	41.0
45	34.863304408310874	35.0	34.0	40.0	30.0	41.0
46	34.75217848788445	35.0	34.0	40.0	29.0	41.0
47	34.724912050889436	35.0	34.0	40.0	29.0	41.0
48	34.66686589823675	35.0	34.0	40.0	29.0	41.0
49	34.69965216042168	35.0	34.0	39.0	29.0	41.0
50	34.55919234159398	35.0	34.0	39.0	29.0	41.0
51	34.008682192498306	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	6.0
13	4.0
14	16.0
15	31.0
16	65.0
17	202.0
18	489.0
19	1021.0
20	1852.0
21	3149.0
22	4968.0
23	7268.0
24	11086.0
25	16473.0
26	22786.0
27	26596.0
28	27684.0
29	29816.0
30	34235.0
31	42344.0
32	55734.0
33	79094.0
34	184521.0
35	516127.0
36	96374.0
37	107637.0
38	182247.0
39	468646.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.27265760163023	23.000260840635743	31.116517668178435	14.610563889555598
2	29.394448832282592	26.13331611750428	31.34372496047041	13.128510089742715
3	27.63442534100618	25.851701946100224	32.67781287469809	13.836059838195503
4	25.590757182358843	27.567783422093772	32.74726624956462	14.094193145982766
5	23.05430327175377	32.20470731441918	30.537878381621823	14.203111032205229
6	20.9715871137437	39.23350339170922	28.086132597634318	11.708776896912761
7	85.96010960513101	5.737973346356275	6.496493749977223	1.8054232985354919
8	88.86824840567019	3.6772802599867864	5.497229414285216	1.9572419200578117
9	85.55276087577894	4.563565717522607	7.464832070173941	2.418841336524509
10	63.2951409190233	16.283223990257785	11.707110848939951	8.714524241778964
11	59.866726574975026	16.5416176180408	14.865260973398941	8.726394833585237
12	49.80134981124197	20.69101422232265	18.68670644703295	10.82092951940243
13	20.24940738152967	50.53706618323508	19.124564679887104	10.088961755348144
14	12.490205460159846	51.68122462856242	25.574096702630744	10.254473208646997
15	10.239010200899354	21.242371973324488	57.599651795973685	10.918966029802474
16	11.43434755739145	15.10277693752268	54.955946047118964	18.506929457966912
17	12.971589196303665	15.587388641613817	28.033652086490797	43.40737007559172
18	21.788002684419798	21.627593503037673	35.1862563537603	21.398147458782233
19	30.402980559823355	23.88415135421065	24.396356977851454	21.31651110811454
20	33.63886223501377	22.01645951269138	22.669289998037186	21.675388254257662
21	21.234562373451944	28.518055534585336	27.283670178730503	22.96371191323222
22	22.173692790125337	25.532028991317286	24.84608580251188	27.448192416045497
23	17.781677949803015	32.27390036928995	22.88040951459172	27.064012166315322
24	19.547897056978318	23.366739330654816	40.0962559216291	16.98910769073776
25	13.822991774408774	24.231470292542404	40.08178212986532	21.863755803183505
26	14.67580008049094	36.61364295446535	27.735325371359487	20.97523159368422
27	16.86628871674217	35.710801145199724	29.85896383270171	17.563946305356396
28	12.344165942543212	29.082845797367955	43.38966831588061	15.183319944208218
29	12.21343324067677	24.013790712094934	42.683055719412536	21.089720327815765
30	16.504339794649177	30.079715189099048	34.4609007175981	18.95504429865368
31	31.183055459092536	26.11004350988409	23.382827106392263	19.324073924631115
32	31.76950434552169	28.068326709924907	25.109165190218423	15.053003754334979
33	27.241446275419595	28.67482023602693	24.886539529851675	19.197193958701796
34	17.273272998100182	29.617855452636597	29.63477625236045	23.474095296902764
35	19.53321500921793	25.579459294543224	31.809853944863182	23.07747175137566
36	32.89268099919145	21.03984301662976	27.930773624169774	18.136702360009018
37	20.899582602918812	30.067376021300422	31.086684996665305	17.946356379115464
38	19.971125306071237	33.59674245970116	22.804344011833106	23.627788222394496
39	20.052241016747427	31.965004662331125	27.509836191039472	20.472918129881975
40	23.352213474891876	26.0369977190762	23.578223295203397	27.032565510828533
41	16.45435835546487	22.787527340107555	27.300070338462852	33.45804396596472
42	24.08392091895041	24.901638089605267	23.391677986247817	27.62276300519651
43	24.13645349409308	27.604748861490496	23.87056265043242	24.388234993984003
44	20.078273016322584	33.228337601713534	25.98946328785196	20.703926094111928
45	16.66011528010692	39.50001900335969	20.81024078037687	23.02962493615652
46	23.91715992967195	33.069334148308464	24.544479055434103	18.46902686658548
47	22.99651223269692	27.135183653153806	26.81488593038106	23.053418183768215
48	26.380151537475925	21.458229313801695	29.515029054314724	22.64659009440765
49	20.9386826662807	20.44371022635865	32.98728128564757	25.630325821713086
50	19.19193549478761	33.37656380729448	26.00987237551888	21.421628322399027
51	17.88309862014783	36.038752275847564	21.683666430122564	24.39448267388204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2388.0
1	2629.5
2	2871.0
3	6633.0
4	10395.0
5	7788.5
6	5182.0
7	6011.5
8	6841.0
9	8011.5
10	9182.0
11	9268.5
12	9355.0
13	9228.0
14	9101.0
15	8233.0
16	7365.0
17	7161.0
18	6957.0
19	6448.0
20	5939.0
21	5684.0
22	5429.0
23	5587.0
24	5745.0
25	6196.5
26	8383.5
27	10119.0
28	11419.5
29	12720.0
30	14281.5
31	15843.0
32	19397.5
33	22952.0
34	26615.5
35	30279.0
36	35021.0
37	39763.0
38	54408.0
39	69053.0
40	110264.0
41	151475.0
42	196906.5
43	242338.0
44	241181.0
45	240024.0
46	223610.5
47	207197.0
48	192330.0
49	177463.0
50	166142.5
51	154822.0
52	141094.5
53	127367.0
54	109861.5
55	92356.0
56	79377.5
57	66399.0
58	59088.0
59	51777.0
60	45428.0
61	39079.0
62	33677.5
63	28276.0
64	23579.5
65	18883.0
66	15497.5
67	12112.0
68	9033.0
69	5954.0
70	5262.5
71	4571.0
72	3733.0
73	2895.0
74	2334.0
75	1375.5
76	978.0
77	710.5
78	443.0
79	348.0
80	253.0
81	175.0
82	97.0
83	64.0
84	31.0
85	23.5
86	16.0
87	8.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1920713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.85661483674569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9956094431602	13.653117918508334
2	8.5042441845186	2.8670553739212896
3	2.565889783958287	1.2975664740517634
4	1.209549769599421	0.8155565836804773
5	0.6918087696854028	0.5830776985634871
6	0.46155661367143314	0.4668169237207187
7	0.3473392007055956	0.4098474186798132
8	0.25693128426856526	0.3464793358740498
9	0.18507719884257695	0.28077975503578034
>10	1.8051287749833178	7.009971294748352
>50	0.9606329397992809	12.220049969425393
>100	2.0040086496730316	57.86333322976698
>500	0.010656286219718382	1.1368925193669597
>1k	9.402605487986807E-4	0.2269664140155425
>5k	6.268403658657871E-4	0.8224890906410534
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9559	0.49767976787786616	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6009	0.312852570894246	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1996	0.10391974230402981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.2063999150315536E-5	0.0	0.0	0.0412346873270499	0.0
2	5.2063999150315536E-5	0.0	0.0	0.1799852450626408	0.0
3	5.2063999150315536E-5	0.0	0.0	0.2582374357855651	0.0
4	5.2063999150315536E-5	0.0	0.0	0.39865404149396605	0.0
5	5.2063999150315536E-5	0.0	0.0	0.6569435412786814	0.0
6	5.2063999150315536E-5	0.0	0.0	0.9719307361380903	0.0
7	5.2063999150315536E-5	0.0	0.0	1.1976802364538586	0.0
8	5.2063999150315536E-5	0.0	0.0	1.7431547555517144	0.0
9	5.2063999150315536E-5	0.0	0.0	1.9987889913797636	0.0
10	5.2063999150315536E-5	0.0	0.0	2.443519672121759	0.0
11	5.2063999150315536E-5	0.0	0.0	2.809113074155275	0.0
12	5.2063999150315536E-5	0.0	0.0	3.1511735485728476	0.0
13	5.2063999150315536E-5	0.0	0.0	3.3042417060747753	0.0
14	5.2063999150315536E-5	0.0	0.0	3.351567881302412	0.0
15	5.2063999150315536E-5	0.0	0.0	3.4240409681196513	0.0
16	1.0412799830063107E-4	0.0	0.0	3.596685189302098	0.0
17	1.0412799830063107E-4	0.0	0.0	3.785469250221142	0.0
18	1.0412799830063107E-4	0.0	0.0	4.1491362842860955	0.0
19	1.0412799830063107E-4	0.0	0.0	4.253993178574832	0.0
20	1.0412799830063107E-4	0.0	0.0	4.382643320475261	0.0
21	1.0412799830063107E-4	0.0	0.0	4.551747189715486	0.0
22	1.0412799830063107E-4	0.0	0.0	4.700494035287937	0.0
23	1.0412799830063107E-4	0.0	0.0	4.889226032207831	0.0
24	1.0412799830063107E-4	0.0	0.0	5.007411310279047	0.0
25	1.0412799830063107E-4	0.0	0.0	5.105604012676542	0.0
26	1.0412799830063107E-4	0.0	0.0	5.215250794887107	0.0
27	1.0412799830063107E-4	0.0	0.0	5.30808090537212	0.0
28	1.0412799830063107E-4	0.0	0.0	5.401015143855433	0.0
29	1.0412799830063107E-4	0.0	0.0	5.498843398258876	0.0
30	1.0412799830063107E-4	0.0	0.0	5.664719299551781	0.0
31	1.0412799830063107E-4	0.0	0.0	5.820442721010375	0.0
32	1.0412799830063107E-4	0.0	0.0	5.941908031028061	0.0
33	1.0412799830063107E-4	0.0	0.0	6.060249501096728	0.0
34	1.0412799830063107E-4	0.0	0.0	6.18598405904474	0.0
35	1.0412799830063107E-4	0.0	0.0	6.378152279908555	0.0
36	1.0412799830063107E-4	0.0	0.0	6.521744789565125	0.0
37	1.0412799830063107E-4	0.0	0.0	6.651592403446012	0.0
38	1.0412799830063107E-4	0.0	0.0	6.772797393467947	0.0
39	1.0412799830063107E-4	0.0	0.0	6.8971262234389	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTC	30	2.1662654E-6	45.000004	36
GTTTACG	60	0.0	45.000004	1
TTCACCG	30	2.1662654E-6	45.000004	15
ACGATTC	30	2.1662654E-6	45.000004	26
GTCACAA	30	2.1662654E-6	45.000004	21
GATATCG	30	2.1662654E-6	45.000004	11
CCGACAC	30	2.1662654E-6	45.000004	45
CACGTTA	30	2.1662654E-6	45.000004	12
ACGAGTT	30	2.1662654E-6	45.000004	29
TAGTCGA	30	2.1662654E-6	45.000004	31
TCACGGT	20	7.034471E-4	45.0	22
TCTGCGT	25	3.8917868E-5	45.0	45
TCGCCTC	20	7.034471E-4	45.0	36
CTATGCG	135	0.0	45.0	1
CACGGTT	20	7.034471E-4	45.0	16
ACGCATC	25	3.8917868E-5	45.0	18
CGACGAT	20	7.034471E-4	45.0	38
TCCGATT	20	7.034471E-4	45.0	37
CCCTAGT	20	7.034471E-4	45.0	15
CGTTGAT	50	2.1827873E-11	45.0	25
>>END_MODULE