FastQCFastQC Report
Thu 26 May 2016
SRR1546835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546835_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences983205
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134421.3671614770063212No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10000.10170818903484014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG253.8903927E-545.01
CTCGACG351.2116834E-745.01
TCACGTA207.0327887E-445.013
CGTTTTT86500.043.855491
ATGCGCG556.002665E-1140.9090928
TCGACGG451.927765E-840.02
TCGGGTA403.457535E-739.3755
GCGATAC403.457535E-739.3759
ACGGGTA2600.038.9423075
GTAGGGC3550.038.6619724
TACGGGT1750.038.5714264
CTTGCGG1400.038.5714262
GCGATCG356.24737E-638.5714269
CGATGTC356.24737E-638.57142610
GCGTAAG356.24737E-638.5714261
GTTTTTT101600.038.511322
CGTTAGG1700.038.3823552
GACGGGT2950.038.1355934
TACGCGG659.094947E-1238.076922
TAGGGTA7850.037.8343935
Produced by FastQC (version 0.11.5)