##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546835_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	983205
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94006844961122	33.0	31.0	34.0	30.0	34.0
2	32.357050665934366	34.0	31.0	34.0	31.0	34.0
3	32.38566524783743	34.0	31.0	34.0	30.0	34.0
4	36.02350577956784	37.0	35.0	37.0	35.0	37.0
5	36.002602712557405	37.0	35.0	37.0	35.0	37.0
6	36.04893892931789	37.0	35.0	37.0	35.0	37.0
7	36.26945957353756	37.0	36.0	37.0	35.0	37.0
8	36.14993922935705	37.0	36.0	37.0	35.0	37.0
9	37.96033685752208	39.0	39.0	39.0	35.0	39.0
10	37.60519423721401	39.0	37.0	39.0	35.0	39.0
11	37.496519037230286	39.0	37.0	39.0	35.0	39.0
12	37.167897844294934	39.0	37.0	39.0	34.0	39.0
13	37.09618238312458	39.0	37.0	39.0	33.0	39.0
14	38.32999120224165	40.0	37.0	41.0	34.0	41.0
15	38.40519932262346	40.0	37.0	41.0	34.0	41.0
16	38.44653251356533	40.0	37.0	41.0	34.0	41.0
17	38.39268819829029	40.0	37.0	41.0	34.0	41.0
18	38.2232047233283	40.0	37.0	41.0	34.0	41.0
19	38.08761651944406	40.0	37.0	41.0	34.0	41.0
20	37.898505398162136	40.0	35.0	41.0	34.0	41.0
21	37.78615446422669	40.0	35.0	41.0	34.0	41.0
22	37.76076301483414	40.0	35.0	41.0	34.0	41.0
23	37.66279463591011	40.0	35.0	41.0	33.0	41.0
24	37.63001001825662	40.0	35.0	41.0	33.0	41.0
25	37.53160429411974	39.0	35.0	41.0	33.0	41.0
26	37.49359696095931	39.0	35.0	41.0	33.0	41.0
27	37.43182754359467	39.0	35.0	41.0	33.0	41.0
28	37.36163261985039	39.0	35.0	41.0	33.0	41.0
29	37.25192609882985	39.0	35.0	41.0	33.0	41.0
30	37.14591972172639	39.0	35.0	41.0	33.0	41.0
31	36.910077755910514	39.0	35.0	41.0	32.0	41.0
32	36.634069192081	39.0	35.0	41.0	31.0	41.0
33	36.35170691768248	39.0	35.0	41.0	30.0	41.0
34	36.05761463784257	39.0	35.0	41.0	29.0	41.0
35	35.856322943841825	39.0	35.0	41.0	27.0	41.0
36	35.73694397404407	39.0	35.0	41.0	26.0	41.0
37	35.476059417924034	39.0	35.0	41.0	24.0	41.0
38	35.562217441937335	39.0	35.0	41.0	25.0	41.0
39	35.45170234081397	39.0	35.0	41.0	24.0	41.0
40	35.36205776008055	39.0	35.0	41.0	24.0	41.0
41	35.269543991334466	39.0	35.0	41.0	23.0	41.0
42	35.254098585747634	39.0	35.0	41.0	23.0	41.0
43	35.15527077262626	38.0	35.0	41.0	23.0	41.0
44	35.009337828835285	38.0	35.0	41.0	23.0	41.0
45	34.970151697763946	38.0	35.0	40.0	23.0	41.0
46	34.802631190850335	38.0	35.0	40.0	23.0	41.0
47	34.7990408917774	38.0	35.0	40.0	23.0	41.0
48	34.65492852457015	38.0	34.0	40.0	23.0	41.0
49	34.620741350989874	38.0	34.0	40.0	23.0	41.0
50	34.51370772117717	37.0	34.0	40.0	23.0	41.0
51	33.98464918302897	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	8.0
13	5.0
14	7.0
15	24.0
16	51.0
17	116.0
18	320.0
19	598.0
20	1103.0
21	1813.0
22	2769.0
23	4417.0
24	6812.0
25	11592.0
26	17570.0
27	20535.0
28	19834.0
29	18163.0
30	18006.0
31	20036.0
32	23624.0
33	30779.0
34	55120.0
35	102974.0
36	61943.0
37	80611.0
38	139529.0
39	344717.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.260011899858114	22.519616966960097	27.22595999816925	18.994411135012534
2	36.310026901816	23.88525282113089	27.36936854470838	12.43535173234473
3	28.016232626969963	23.582264125996105	35.08281589292162	13.318687354112315
4	24.530794696935025	25.882598237397087	34.96737709836707	14.619229967300818
5	22.56345319643411	29.522632614764976	33.45558657655321	14.4583276122477
6	19.963588468325526	38.41823424413017	31.267741722224763	10.350435565319541
7	82.01961950966482	4.031407488773959	12.041639332590863	1.9073336689703573
8	82.80012815231818	3.280292512751664	11.832527295935233	2.0870520389949196
9	77.39525327881775	4.741839189182317	14.462395939809095	3.4005115921908455
10	35.80952090357555	22.733712704878435	28.980222842642174	12.476543548903841
11	32.53624625586729	23.743878438372466	27.890521305322896	15.829354000437345
12	29.080405408841493	23.12132261329021	31.28869360916594	16.509578368702357
13	22.902243174109163	27.73531460885573	32.58842255684216	16.77401966019294
14	18.777060735045083	30.407493859368085	33.436363728825626	17.379081676761203
15	18.65256991166644	24.26320045158436	40.64544016761509	16.438789469134107
16	20.70076942245005	22.21144115418453	39.20209925702168	17.885690166343743
17	20.76087896216964	22.224358094191953	32.99383139833504	24.02093154530337
18	22.711642027857874	23.934479584623755	34.401777859144325	18.952100528374043
19	24.956850300801968	25.069542974252574	31.414404930813006	18.559201794132456
20	25.811605921450763	24.566494271286253	30.880233522002026	18.74166628526096
21	23.917290900676868	25.825641651537573	32.18260688259315	18.074460565192407
22	22.39909276295381	23.61369195640787	33.05231360703007	20.93490167360825
23	20.81549625968135	26.556313281563863	32.17263948006774	20.455550978687047
24	20.53793461180527	25.63585417079856	35.414791421931334	18.41141979546483
25	20.104149185571675	26.132495257855687	33.887032714439	19.876322842133636
26	19.106188434761826	28.94971038593172	32.024552356833006	19.91954882247344
27	19.610152511429458	27.290951530962516	33.58536622576167	19.51352973184636
28	18.23465096292228	26.83590909322064	36.58830050701532	18.341139436841758
29	19.521361262402042	25.945657314598687	35.532467796644646	19.000513626354625
30	20.828311491499736	25.925112260413645	34.343804191394476	18.902772056692143
31	24.220279595811654	25.916060231589544	30.578973866080826	19.28468630651797
32	24.536083522764834	27.05254753586485	29.974522098646773	18.436846842723543
33	23.833585061101196	26.72453862622749	29.909835690420618	19.5320406222507
34	20.973245660874383	27.54532371173865	31.62839896054231	19.853031666844657
35	21.302780193347267	27.63279275430861	31.015607121607395	20.048819930736723
36	24.76838502652041	27.73694193988029	28.867428461002536	18.627244572596762
37	22.108715883259343	29.560162936518836	28.947167681205855	19.383953499015973
38	21.967544916878985	30.112438402978015	27.56586876592369	20.354147914219315
39	21.428796639561433	29.061691102059083	28.639093576619324	20.87041868176016
40	22.525312625546047	27.427749045214377	28.45632396092371	21.590614368315865
41	20.059600998774414	27.565563641356583	29.04165458881922	23.33318077104978
42	21.840307972396396	28.54013150868842	27.599127343738083	22.020433175177097
43	21.838782349560876	28.139096119324048	28.217411424880872	21.804710106234204
44	21.56132240987383	29.207337228756973	29.000157647693005	20.23118271367619
45	20.754979887205618	29.867118251025982	27.832344221194973	21.54555764057343
46	21.516875931265606	29.573181584715293	29.114274235790095	19.795668248229006
47	21.73402291485499	27.829089559145853	29.61895026978097	20.817937256218183
48	22.213475317965226	26.737455566234914	30.189329793888355	20.8597393219115
49	21.21754873093607	26.466301534268034	30.879216440111673	21.43693329468422
50	20.23514933304855	28.596071012657585	30.193703246016852	20.975076408277012
51	20.02969879119817	30.08619769020703	28.394790506557637	21.489313012037165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	658.0
1	1029.0
2	1400.0
3	12308.5
4	23217.0
5	15982.0
6	8747.0
7	8906.5
8	9066.0
9	9262.5
10	9459.0
11	9446.5
12	9434.0
13	9062.5
14	8691.0
15	8230.0
16	7769.0
17	7254.0
18	6739.0
19	6446.0
20	6153.0
21	6090.0
22	6027.0
23	6438.0
24	6849.0
25	7334.0
26	9352.0
27	10885.0
28	12466.5
29	14048.0
30	15316.5
31	16585.0
32	19351.5
33	22118.0
34	25241.5
35	28365.0
36	31164.0
37	33963.0
38	38177.5
39	42392.0
40	48945.5
41	55499.0
42	60748.5
43	65998.0
44	67977.0
45	69956.0
46	70568.0
47	71180.0
48	71506.0
49	71832.0
50	71723.5
51	71615.0
52	67934.0
53	64253.0
54	58014.0
55	51775.0
56	47991.5
57	44208.0
58	40448.5
59	36689.0
60	33408.5
61	30128.0
62	26763.5
63	23399.0
64	20161.0
65	16923.0
66	13750.5
67	10578.0
68	8859.5
69	7141.0
70	5866.0
71	4591.0
72	3786.0
73	2981.0
74	2443.5
75	1491.0
76	1076.0
77	799.0
78	522.0
79	419.0
80	316.0
81	229.0
82	142.0
83	102.0
84	62.0
85	42.5
86	23.0
87	15.5
88	8.0
89	8.0
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	983205.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.61731251857381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66022876389641	21.365750992446376
2	5.922989245766138	3.390000685804827
3	2.6373734807004605	2.264236233762116
4	1.826008677809427	2.090218439780005
5	1.3695801874128306	1.9596852121219932
6	1.1392229553844617	1.9560899605542243
7	1.0245653737964184	2.052421524834905
8	0.8347582889952077	1.9110831066916647
9	0.8138202333469761	2.096041315646617
>10	9.610227149732454	55.79300302183413
>50	0.14030599444441968	2.4183977639652294
>100	0.020198281517765298	1.2123980711809006
>500	3.6068359853151226E-4	0.10321795259500097
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.6068359853151226E-4	1.387455718782003
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13442	1.3671614770063212	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1000	0.10170818903484014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0170818903484015E-4	0.0	0.0	0.016578434812678945	0.0
2	1.0170818903484015E-4	0.0	0.0	0.06550007373843705	0.0
3	1.0170818903484015E-4	0.0	0.0	0.13089843928783926	0.0
4	1.0170818903484015E-4	0.0	0.0	0.18510890404340904	0.0
5	1.0170818903484015E-4	0.0	0.0	0.3124475567150289	0.0
6	1.0170818903484015E-4	0.0	0.0	0.5972304860125813	0.0
7	1.0170818903484015E-4	0.0	0.0	0.7904760451787776	0.0
8	1.0170818903484015E-4	0.0	0.0	1.1747295833524036	0.0
9	1.0170818903484015E-4	0.0	0.0	1.4193377779811942	0.0
10	1.0170818903484015E-4	0.0	0.0	1.7246657614637844	0.0
11	1.0170818903484015E-4	0.0	0.0	1.9363205028452866	0.0
12	1.0170818903484015E-4	0.0	0.0	2.119903784053173	0.0
13	1.0170818903484015E-4	0.0	0.0	2.2117462787516335	0.0
14	1.0170818903484015E-4	0.0	0.0	2.2593457112199387	0.0
15	1.0170818903484015E-4	0.0	0.0	2.3017580260474673	0.0
16	1.0170818903484015E-4	0.0	0.0	2.381293829872712	0.0
17	1.0170818903484015E-4	0.0	0.0	2.467237249607152	0.0
18	1.0170818903484015E-4	0.0	0.0	2.603627931102873	0.0
19	1.0170818903484015E-4	0.0	0.0	2.6635340544443937	0.0
20	1.0170818903484015E-4	0.0	0.0	2.731271708341597	0.0
21	1.0170818903484015E-4	0.0	0.0	2.80592551909317	0.0
22	1.0170818903484015E-4	0.0	0.0	2.8881057358333204	0.0
23	1.0170818903484015E-4	0.0	0.0	2.9778123585620495	0.0
24	1.0170818903484015E-4	0.0	0.0	3.05073713010003	0.0
25	1.0170818903484015E-4	0.0	0.0	3.113592790923561	0.0
26	1.0170818903484015E-4	0.0	0.0	3.1783809073387546	0.0
27	2.034163780696803E-4	0.0	0.0	3.2553740064381285	0.0
28	2.034163780696803E-4	0.0	0.0	3.328705610732248	0.0
29	2.034163780696803E-4	0.0	0.0	3.410987535661434	0.0
30	2.034163780696803E-4	0.0	0.0	3.5276468284843956	0.0
31	2.034163780696803E-4	0.0	0.0	3.615115871054358	0.0
32	2.034163780696803E-4	0.0	0.0	3.7171291846563026	0.0
33	3.051245671045204E-4	0.0	0.0	3.8085648465986237	0.0
34	3.051245671045204E-4	0.0	0.0	3.9042722524804083	0.0
35	3.051245671045204E-4	0.0	0.0	4.003437736789378	0.0
36	3.051245671045204E-4	0.0	0.0	4.0987383099150225	0.0
37	3.051245671045204E-4	0.0	0.0	4.200446498949863	0.0
38	3.051245671045204E-4	0.0	0.0	4.3109015922416996	0.0
39	3.051245671045204E-4	0.0	0.0	4.463667292172029	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	25	3.8903927E-5	45.0	1
CTCGACG	35	1.2116834E-7	45.0	1
TCACGTA	20	7.0327887E-4	45.0	13
CGTTTTT	8650	0.0	43.85549	1
ATGCGCG	55	6.002665E-11	40.90909	28
TCGACGG	45	1.927765E-8	40.0	2
TCGGGTA	40	3.457535E-7	39.375	5
GCGATAC	40	3.457535E-7	39.375	9
ACGGGTA	260	0.0	38.942307	5
GTAGGGC	355	0.0	38.661972	4
TACGGGT	175	0.0	38.571426	4
CTTGCGG	140	0.0	38.571426	2
GCGATCG	35	6.24737E-6	38.571426	9
CGATGTC	35	6.24737E-6	38.571426	10
GCGTAAG	35	6.24737E-6	38.571426	1
GTTTTTT	10160	0.0	38.51132	2
CGTTAGG	170	0.0	38.382355	2
GACGGGT	295	0.0	38.135593	4
TACGCGG	65	9.094947E-12	38.07692	2
TAGGGTA	785	0.0	37.834393	5
>>END_MODULE