FastQCFastQC Report
Thu 26 May 2016
SRR1546834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546834_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3187204
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104140.32674406784128035No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC100800.31626466332246067No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT207.0351706E-445.00000418
ACCGGTA800.045.00000441
CGAACCG207.0351706E-445.00000416
TCCGATT551.8189894E-1245.00000438
CACGCTA406.8212103E-945.00000437
TATGCGT406.8212103E-945.00000420
CCGTAGA207.0351706E-445.00000432
TGCTCGA207.0351706E-445.00000418
CTTACGC207.0351706E-445.00000433
GTCGACG351.2128294E-745.01
TTTACGT453.8562575E-1045.032
ATTACGA253.892368E-545.040
CTAACCG351.2128294E-745.01
CGTAGGT253.892368E-545.03
TAACACG253.892368E-545.031
GATATCG253.892368E-545.011
GTTCACG453.8562575E-1045.01
CCGAATA351.2128294E-745.036
ACCGTAG351.2128294E-745.042
CACGTTA351.2128294E-745.027