FastQCFastQC Report
Thu 26 May 2016
SRR1546833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546833_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2348376
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244271.0401656293540726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32370.13783993704585637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136200.042.802861
TATAGCG1600.040.7812541
ATACGAC356.2510753E-638.5714261
CGAAGTA356.2510753E-638.57142630
TACGTAG1000.038.2500041
CGGTCTA3150.037.85714331
GGGCGAT32950.037.8300487
GACCGAT7200.037.81259
AGGGCGA18250.037.232886
TATCGCG850.037.0588231
GTTTTTT165700.037.0564272
GGCGATT10100.036.9801988
TAAGGGA18450.036.7073174
AGGGATC14700.036.4285746
TAGGGAC16000.036.421885
AAGGGAT19300.036.373065
TAGGGAT20800.036.1298075
GCGAACC250.002107554836.00000413
TATCGCC250.002107554836.00000421
AGGGATT23250.035.8064546
Produced by FastQC (version 0.11.5)