##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546833_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2348376
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94144251176132	33.0	31.0	34.0	30.0	34.0
2	32.334791362200946	34.0	31.0	34.0	31.0	34.0
3	32.37709804562813	34.0	31.0	34.0	30.0	34.0
4	35.995662960275524	37.0	35.0	37.0	35.0	37.0
5	35.95740545806974	37.0	35.0	37.0	35.0	37.0
6	36.013763554047564	37.0	35.0	37.0	35.0	37.0
7	36.23747389685468	37.0	35.0	37.0	35.0	37.0
8	36.09690313646537	37.0	35.0	37.0	35.0	37.0
9	37.88479655728044	39.0	38.0	39.0	35.0	39.0
10	37.64807296616896	39.0	37.0	39.0	35.0	39.0
11	37.4546601566359	39.0	37.0	39.0	35.0	39.0
12	36.963285266073235	39.0	35.0	39.0	33.0	39.0
13	36.840249176452154	39.0	35.0	39.0	33.0	39.0
14	38.03646988386868	40.0	36.0	41.0	33.0	41.0
15	38.138781864573644	40.0	36.0	41.0	33.0	41.0
16	38.190652178356444	40.0	36.0	41.0	34.0	41.0
17	38.13617921491277	40.0	36.0	41.0	34.0	41.0
18	37.992787781854354	40.0	36.0	41.0	34.0	41.0
19	37.90860109284033	40.0	36.0	41.0	34.0	41.0
20	37.76579091252849	40.0	35.0	41.0	34.0	41.0
21	37.63880060092592	40.0	35.0	41.0	33.0	41.0
22	37.59874440890215	40.0	35.0	41.0	33.0	41.0
23	37.526535358903345	39.0	35.0	41.0	33.0	41.0
24	37.49607941828736	39.0	35.0	41.0	33.0	41.0
25	37.389920949626465	39.0	35.0	41.0	33.0	41.0
26	37.35101576578878	39.0	35.0	41.0	33.0	41.0
27	37.27987042960753	39.0	35.0	41.0	33.0	41.0
28	37.20271924087114	39.0	35.0	41.0	33.0	41.0
29	37.09335344936245	39.0	35.0	41.0	33.0	41.0
30	37.030922646118	39.0	35.0	41.0	33.0	41.0
31	36.820838315499735	39.0	35.0	41.0	32.0	41.0
32	36.62762905088453	39.0	35.0	41.0	31.0	41.0
33	36.452660902683384	39.0	35.0	41.0	31.0	41.0
34	36.239699690339194	39.0	35.0	41.0	30.0	41.0
35	36.07709497967957	39.0	35.0	41.0	30.0	41.0
36	35.98961665423254	39.0	35.0	41.0	30.0	41.0
37	35.72766243565766	39.0	35.0	41.0	28.0	41.0
38	35.80995547561378	39.0	35.0	41.0	29.0	41.0
39	35.70952011091921	38.0	35.0	41.0	28.0	41.0
40	35.61442460662177	38.0	35.0	41.0	28.0	41.0
41	35.53703282608918	38.0	35.0	41.0	27.0	41.0
42	35.52137988124559	38.0	35.0	41.0	28.0	41.0
43	35.393978221545446	38.0	35.0	41.0	27.0	41.0
44	35.256610099915854	38.0	35.0	40.0	27.0	41.0
45	35.2139661621478	38.0	35.0	40.0	27.0	41.0
46	35.04351688145339	37.0	35.0	40.0	26.0	41.0
47	35.01915877184914	37.0	35.0	40.0	26.0	41.0
48	34.88190306833318	37.0	34.0	40.0	25.0	41.0
49	34.851114131638205	37.0	34.0	40.0	26.0	41.0
50	34.73543972515475	37.0	34.0	40.0	26.0	41.0
51	34.18819516125186	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	10.0
12	8.0
13	22.0
14	38.0
15	58.0
16	120.0
17	323.0
18	765.0
19	1543.0
20	2746.0
21	4444.0
22	6858.0
23	10064.0
24	15019.0
25	23890.0
26	33649.0
27	39412.0
28	40012.0
29	40199.0
30	43002.0
31	49454.0
32	60496.0
33	80438.0
34	150473.0
35	291458.0
36	151003.0
37	199030.0
38	329569.0
39	773901.0
40	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.30421704190471	22.699601767349012	26.620183479987876	16.375997710758412
2	34.312605817807714	23.964603623951188	28.55462668669753	13.16816387154357
3	29.281895233131323	24.026646499538405	32.89192190688374	13.799536360446538
4	26.39130190395405	26.164464293622487	32.45089372400331	14.99334007842015
5	22.72016917222796	30.712458311616196	31.39250273380413	15.174869782351719
6	21.16415769876715	39.638882359554	28.648606526382487	10.548353415296358
7	82.7580847360048	4.91228832180196	9.779694563391892	2.5499323788013504
8	83.15069648131305	4.244124450258392	9.864817218367076	2.7403618500614892
9	78.00356501684568	5.950111907122198	11.974360153569956	4.071962922462161
10	42.95683485097786	26.61311476526757	17.709004009579385	12.721046374175174
11	35.610779534452746	23.61632038481061	25.300803619181938	15.472096461554708
12	31.71481057547854	22.017130135889655	29.69571312260047	16.572346166031334
13	23.733422586502332	28.921986939059163	30.237534364173367	17.107056110265138
14	18.1813304172756	32.47090755483789	32.049978368029656	17.297783659856854
15	17.291055606086932	23.791803356873004	41.67437412066892	17.242766916371146
16	19.956514629684513	21.615831536346818	39.43078110149312	18.996872732475552
17	21.23033108837767	21.123959706622788	30.61600868004102	27.02970052495852
18	23.731293455562483	22.985118226382827	32.623566243225106	20.660022074829584
19	26.395602748452546	24.819109035350387	28.774097503977213	20.011190712219847
20	27.786265913124648	24.458263923664695	27.544354055738946	20.211116107471717
21	24.89971793273309	25.4130514023308	30.05327937263879	19.633951292297315
22	23.549593421155727	23.267951980432436	29.97944111164481	23.203013486767023
23	21.695673946591178	26.344886849465333	29.314726432223804	22.644712771719693
24	21.990345668666347	24.14724047597148	33.46278449447618	20.39962936088599
25	20.96844798277618	25.63839862100447	32.0280057367304	21.36514765948894
26	19.811946638868733	28.48244914783663	29.776236854745576	21.929367358549058
27	20.03256718685594	27.136369985044983	31.6036699404184	21.227392887680676
28	19.306916779936433	25.882269278854835	35.29898959962118	19.51182434158755
29	20.14583695285593	25.184340156772166	34.318652549676884	20.351170340695017
30	21.579806640844566	25.7469842989368	32.569358569496536	20.1038504907221
31	26.126650928130758	24.59244175549401	28.39460120525844	20.886306111116788
32	26.021855103271367	26.397093140110446	28.351720508129873	19.229331248488318
33	25.51976344503606	25.585894252027785	28.148303338136653	20.746038964799503
34	21.53364708206863	27.058273462171307	29.685195215757616	21.72288424000245
35	21.32784528542278	26.870271200182593	30.233914841575626	21.567968672819006
36	25.768573686666873	25.94567479824355	27.889954589895318	20.39579692519426
37	22.531443005719698	28.897672263726083	28.1292689075344	20.441615823019823
38	21.74498461915809	29.592237358923786	26.471442392529987	22.19133562938814
39	21.840838094070115	27.664905449553224	27.963665103032902	22.530591353343755
40	22.62589125421142	25.622685634668386	28.08268352257049	23.668739588549702
41	20.29938987623788	25.08989190828045	28.64694580424941	25.963772411232274
42	23.03970914368057	26.616308461677345	26.51594122917284	23.828041165469244
43	22.43141643416557	26.437333714873596	27.67997969660736	23.451270154353477
44	21.678129907646817	28.098950082950942	28.071058467638917	22.151861541763328
45	20.800331803765665	29.769508800975654	27.02399445403973	22.406164941218954
46	22.975111310965534	28.213923153702815	28.26915281028251	20.54181272504914
47	22.26670686465881	27.149655762109642	28.693318276119328	21.89031909711222
48	22.76811720099337	25.311449273881188	29.823460978991438	22.09697254613401
49	22.129377919038518	24.329792162754178	30.210579566474877	23.33025035173243
50	20.617183960319814	27.777621641508855	29.125787352621558	22.479407045549777
51	20.676927374491992	28.96018354812006	27.85209864178479	22.510790435603155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1799.0
1	2989.0
2	4179.0
3	22365.5
4	40552.0
5	27363.5
6	14175.0
7	14222.5
8	14270.0
9	14669.5
10	15069.0
11	14903.0
12	14737.0
13	14260.0
14	13783.0
15	13072.0
16	12361.0
17	11760.0
18	11159.0
19	11472.0
20	11785.0
21	11947.0
22	12109.0
23	12858.5
24	13608.0
25	14604.0
26	17733.5
27	19867.0
28	23216.0
29	26565.0
30	31910.5
31	37256.0
32	42838.0
33	48420.0
34	55618.5
35	62817.0
36	66337.0
37	69857.0
38	79978.5
39	90100.0
40	108310.5
41	126521.0
42	145020.5
43	163520.0
44	168819.5
45	174119.0
46	177396.5
47	180674.0
48	179358.0
49	178042.0
50	174719.0
51	171396.0
52	159277.5
53	147159.0
54	134138.0
55	121117.0
56	116530.0
57	111943.0
58	105863.5
59	99784.0
60	93970.5
61	88157.0
62	80829.5
63	73502.0
64	66058.5
65	58615.0
66	48084.0
67	37553.0
68	31019.0
69	24485.0
70	20286.5
71	16088.0
72	13601.5
73	11115.0
74	9087.0
75	5520.5
76	3982.0
77	2774.5
78	1567.0
79	1312.0
80	1057.0
81	748.5
82	440.0
83	359.0
84	278.0
85	169.5
86	61.0
87	51.5
88	42.0
89	26.5
90	11.0
91	11.5
92	12.0
93	8.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2348376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.06545847798084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60888147368128	17.786719271139933
2	6.142450302826634	2.7107196422016386
3	2.0352633698209113	1.3472705813561603
4	1.1931625148081169	1.053107117119268
5	0.7644146034183251	0.8433579345844622
6	0.5663381941984742	0.749790714514865
7	0.440040124152626	0.6796780961694713
8	0.3675735935017198	0.6488543892011525
9	0.3221862264278179	0.6398268121278315
>10	5.086114862870555	28.738558577545188
>50	2.0946974544155186	32.25480680871186
>100	0.37336634836644933	10.468319875817132
>500	0.004133198633725818	0.6206005653780563
>1k	0.0011809138953502336	0.39754916553329095
>5k	0.0	0.0
>10k+	1.9681898255837226E-4	1.0608404485997047
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24427	1.0401656293540726	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3237	0.13783993704585637	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.258261879698992E-5	0.0	0.0	0.033512520993231064	0.0
2	4.258261879698992E-5	0.0	0.0	0.11050189577818884	0.0
3	4.258261879698992E-5	0.0	0.0	0.18229619106991385	0.0
4	4.258261879698992E-5	0.0	0.0	0.25788033943457095	0.0
5	4.258261879698992E-5	0.0	0.0	0.45039635901576236	0.0
6	4.258261879698992E-5	0.0	0.0	0.7492837603518346	0.0
7	4.258261879698992E-5	0.0	0.0	0.9513808691623488	0.0
8	4.258261879698992E-5	0.0	0.0	1.4167237273758546	0.0
9	4.258261879698992E-5	0.0	0.0	1.6963212023968905	0.0
10	4.258261879698992E-5	0.0	0.0	2.101196741918671	0.0
11	4.258261879698992E-5	0.0	0.0	2.386372540002112	0.0
12	4.258261879698992E-5	0.0	0.0	2.652939733671269	0.0
13	4.258261879698992E-5	0.0	0.0	2.7695309439374274	0.0
14	4.258261879698992E-5	0.0	0.0	2.829402105965995	0.0
15	8.516523759397984E-5	0.0	0.0	2.8906784944148636	0.0
16	1.2774785639096977E-4	0.0	0.0	3.008078774438165	0.0
17	1.2774785639096977E-4	0.0	0.0	3.135400804641165	0.0
18	1.2774785639096977E-4	0.0	0.0	3.3383495658276185	0.0
19	1.2774785639096977E-4	0.0	0.0	3.4290079612464104	0.0
20	1.2774785639096977E-4	0.0	0.0	3.531887568259938	0.0
21	1.2774785639096977E-4	0.0	0.0	3.6531202839749684	0.0
22	1.2774785639096977E-4	0.0	0.0	3.7813365491727047	0.0
23	1.2774785639096977E-4	0.0	0.0	3.9150033895764564	0.0
24	1.2774785639096977E-4	0.0	0.0	4.026314355111788	0.0
25	1.2774785639096977E-4	0.0	0.0	4.115695271966669	0.0
26	1.2774785639096977E-4	0.0	0.0	4.212059738304258	0.0
27	1.2774785639096977E-4	0.0	0.0	4.318771781009515	0.0
28	1.2774785639096977E-4	0.0	0.0	4.421310727072666	0.0
29	1.2774785639096977E-4	0.0	0.0	4.536070884730554	0.0
30	1.2774785639096977E-4	0.0	0.0	4.693371078566635	0.0
31	1.7033047518795968E-4	0.0	0.0	4.818947221398958	0.0
32	1.7033047518795968E-4	0.0	0.0	4.942649729004214	0.0
33	1.7033047518795968E-4	0.0	0.0	5.063925027338041	0.0
34	1.7033047518795968E-4	0.0	0.0	5.19282261443653	0.0
35	1.7033047518795968E-4	0.0	0.0	5.33994556238013	0.0
36	2.129130939849496E-4	0.0	0.0	5.463775817841777	0.0
37	2.129130939849496E-4	0.0	0.0	5.598422058477858	0.0
38	2.129130939849496E-4	0.0	0.0	5.736815569568076	0.0
39	2.129130939849496E-4	0.0	0.0	5.914981246614682	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13620	0.0	42.80286	1
TATAGCG	160	0.0	40.781254	1
ATACGAC	35	6.2510753E-6	38.571426	1
CGAAGTA	35	6.2510753E-6	38.571426	30
TACGTAG	100	0.0	38.250004	1
CGGTCTA	315	0.0	37.857143	31
GGGCGAT	3295	0.0	37.830048	7
GACCGAT	720	0.0	37.8125	9
AGGGCGA	1825	0.0	37.23288	6
TATCGCG	85	0.0	37.058823	1
GTTTTTT	16570	0.0	37.056427	2
GGCGATT	1010	0.0	36.980198	8
TAAGGGA	1845	0.0	36.707317	4
AGGGATC	1470	0.0	36.428574	6
TAGGGAC	1600	0.0	36.42188	5
AAGGGAT	1930	0.0	36.37306	5
TAGGGAT	2080	0.0	36.129807	5
GCGAACC	25	0.0021075548	36.000004	13
TATCGCC	25	0.0021075548	36.000004	21
AGGGATT	2325	0.0	35.806454	6
>>END_MODULE