FastQCFastQC Report
Thu 26 May 2016
SRR1546832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546832_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2226806
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187960.8440789184149854No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31420.1410989551851396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119300.043.2271581
GCGATAC900.042.59
CGGTCTA2450.041.3265331
CGTTGTA451.9297659E-840.035
GCGCGAC5500.038.8636369
CACGACG2550.038.8235326
CGCTATT356.250928E-638.57142629
GCGATCG700.038.5714269
TCACGAC2700.038.33333625
TGCGTAG1650.038.1818161
TAATGCG659.094947E-1238.076921
AGGGCGA19150.037.950396
CGACTCG301.1402654E-437.50000412
GGGCGAT35200.037.3934677
ATAGGGC9050.037.292824
TACGGGA5750.037.1739124
TCGTTAG552.752131E-936.818181
GTCGACG552.752131E-936.8181811
GTTTTTT148850.036.761842
CGCATCG1350.036.66666821
Produced by FastQC (version 0.11.5)