FastQCFastQC Report
Thu 26 May 2016
SRR1546831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546831_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2202022
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.3665721777529925No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44780.20335854955127608No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29230.13274163473389458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG453.8562575E-1045.01
AAATCGT207.034698E-445.036
TCCGATT253.8919745E-545.029
ATTCGCA207.034698E-445.017
ACGATAT253.8919745E-545.024
ACCTAGT406.8193913E-945.035
TCGTGAC351.2126111E-745.017
CGGATAT351.2126111E-745.012
TCGCTTA207.034698E-445.041
TCGCTGA406.8193913E-945.042
ACGTCCA207.034698E-445.021
TCGACCC207.034698E-445.017
CAATACG253.8919745E-545.01
TACGTCA351.2126111E-745.032
AGTCGAT207.034698E-445.030
GTACGAT207.034698E-445.027
ATCGTTC302.166411E-644.99999632
CGTAAAT302.166411E-644.99999618
ATACCGG600.044.9999962
TACCGGG2500.043.23