##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546831_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2202022
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988107748242296	33.0	31.0	34.0	30.0	34.0
2	32.37717243515278	34.0	31.0	34.0	31.0	34.0
3	32.443612734114375	34.0	31.0	34.0	31.0	34.0
4	36.022248642384135	37.0	35.0	37.0	35.0	37.0
5	35.971224174872006	37.0	35.0	37.0	35.0	37.0
6	36.03454870114831	37.0	35.0	37.0	35.0	37.0
7	36.26439427035697	37.0	36.0	37.0	35.0	37.0
8	36.14181874658836	37.0	36.0	37.0	35.0	37.0
9	37.979053796919374	39.0	39.0	39.0	35.0	39.0
10	37.70395709034696	39.0	38.0	39.0	35.0	39.0
11	37.438807605010304	39.0	37.0	39.0	35.0	39.0
12	36.574130957819676	39.0	35.0	39.0	33.0	39.0
13	36.29689803280803	39.0	35.0	39.0	33.0	39.0
14	37.35903773895084	40.0	35.0	41.0	33.0	41.0
15	37.55181101732862	40.0	35.0	41.0	33.0	41.0
16	37.672031887056534	40.0	35.0	41.0	34.0	41.0
17	37.61756240400868	39.0	35.0	41.0	34.0	41.0
18	37.5752989752146	39.0	35.0	41.0	34.0	41.0
19	37.53633251620556	39.0	35.0	41.0	34.0	41.0
20	37.41011579357518	39.0	35.0	41.0	34.0	41.0
21	37.25165325323725	39.0	35.0	41.0	33.0	41.0
22	37.208129164922056	39.0	35.0	41.0	33.0	41.0
23	37.1615501570829	38.0	35.0	41.0	33.0	41.0
24	37.13777927740958	38.0	35.0	41.0	33.0	41.0
25	37.026290382203264	38.0	35.0	41.0	33.0	41.0
26	36.953824712014686	38.0	35.0	41.0	33.0	41.0
27	36.89741973513434	38.0	35.0	41.0	33.0	41.0
28	36.88539351559612	38.0	35.0	41.0	33.0	41.0
29	36.85267903772078	38.0	35.0	41.0	33.0	41.0
30	36.850822562172404	38.0	35.0	41.0	33.0	41.0
31	36.67039657187803	38.0	35.0	41.0	32.0	41.0
32	36.519765470099756	38.0	35.0	41.0	32.0	41.0
33	36.445018260489675	38.0	35.0	41.0	32.0	41.0
34	36.34604876790513	38.0	35.0	41.0	32.0	41.0
35	36.24466921765541	38.0	35.0	41.0	31.0	41.0
36	36.14163982012895	38.0	35.0	41.0	31.0	41.0
37	35.86583376551188	38.0	35.0	41.0	30.0	41.0
38	35.96554348684981	38.0	35.0	41.0	31.0	41.0
39	35.921668357536845	37.0	35.0	41.0	31.0	41.0
40	35.79239489887022	37.0	35.0	40.0	30.0	41.0
41	35.76973981186382	37.0	35.0	40.0	30.0	41.0
42	35.76291199633791	37.0	35.0	40.0	30.0	41.0
43	35.64600898628624	37.0	35.0	40.0	30.0	41.0
44	35.53618764935137	37.0	35.0	40.0	30.0	41.0
45	35.484068279063514	36.0	35.0	40.0	30.0	41.0
46	35.34480127809804	36.0	35.0	40.0	30.0	41.0
47	35.33076372533971	36.0	35.0	40.0	30.0	41.0
48	35.212590973205536	36.0	35.0	40.0	29.0	41.0
49	35.21541564979823	36.0	35.0	40.0	29.0	41.0
50	35.08690694280075	35.0	34.0	40.0	29.0	41.0
51	34.54553769217564	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	3.0
13	9.0
14	13.0
15	43.0
16	114.0
17	227.0
18	570.0
19	1144.0
20	2174.0
21	3455.0
22	5308.0
23	8129.0
24	11482.0
25	17024.0
26	23294.0
27	28347.0
28	30355.0
29	33007.0
30	38004.0
31	46088.0
32	59568.0
33	81929.0
34	171438.0
35	423052.0
36	125310.0
37	159309.0
38	268389.0
39	663783.0
40	445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.73518611530675	24.57032672698093	29.08168038284813	13.612806774864191
2	29.448752101477645	25.99787831365899	31.27493730762	13.278432277243372
3	28.83381728247947	25.318911436852133	32.18650858165813	13.660762699010274
4	26.864127606354522	27.309445591370114	30.690020354020074	15.136406448255284
5	23.092775639843744	32.51384409420069	29.324003120768094	15.069377145187469
6	20.810555026244064	41.054085744829074	26.84446386094235	11.290895367984517
7	86.45463124346622	5.056806880221905	6.3611535216269415	2.1274083546849214
8	87.69980499740693	4.039923306851612	5.931321303783522	2.3289503919579366
9	83.58145377294142	5.462025356694893	7.647107976214588	3.3094128941491046
10	53.52889299016994	23.14781596187504	12.211867093062649	11.111423954892368
11	46.829641120751745	20.148663364852847	19.422421756004255	13.599273758391151
12	41.49676978704118	21.285709225430082	22.517486201318608	14.700034786210129
13	21.93524860332912	39.744607456237944	23.335461680219364	14.984682260213567
14	15.805291681917803	42.195264170839344	26.433659609213713	15.56578453802914
15	15.018060673326605	22.8446854754403	47.45311354745775	14.684140303775347
16	16.376721031851634	18.50194957180264	45.78968784144754	19.33164155489818
17	17.06150074794893	18.684009514891315	28.75139303785339	35.50309669930636
18	22.925701923050724	22.692688810556845	32.48473448494157	21.89687478145087
19	29.240307317547238	25.042983221784343	24.692623416114827	21.0240860445536
20	31.671527350771246	23.65335132891497	23.36284560281414	21.31227571749964
21	23.594814220748024	27.538099074396165	26.558363177116306	22.308723527739506
22	22.93551108935333	24.498756143217463	25.561733715648614	27.003999051780593
23	19.94298876214679	30.27358491422883	24.373462208824435	25.409964114799944
24	21.68311669910655	24.325506284678355	35.380709184558555	18.610667831656542
25	19.01416062146518	25.644793739572087	33.22310131324755	22.117944325715182
26	17.62566404876972	33.28518062035711	26.97221008691103	22.116945243962142
27	19.40852543707556	30.484527402541843	29.719639494973254	20.387307665409338
28	16.295341281785557	27.18269844715448	39.12613043829716	17.395829832762796
29	16.735618445228976	24.81051506297394	36.69840719120881	21.755459300588278
30	19.35693648837296	28.325602559829104	31.370622091877376	20.946838859920565
31	29.605471698284575	25.43884666002429	23.134464596629826	21.82121704506131
32	29.32977054725157	27.09600539867449	25.616637799259045	17.957586254814892
33	26.488518280017182	27.32633915555794	25.237395448365184	20.947747116059695
34	19.676279346891175	28.265930131488243	28.307210373011714	23.75058014860887
35	20.5442089134441	25.366504058542557	30.631891961115738	23.457395066897607
36	29.39180444155417	24.22818663937054	26.823710208163227	19.556298710912063
37	21.248788613374433	30.487933363063586	28.056940393874356	20.206337629687624
38	21.68179972770481	32.32342819463202	22.78319653482118	23.211575542841988
39	21.52303655458483	29.29825405922375	26.329164740406775	22.849544645784647
40	23.406941438368918	26.314859706215472	24.620825768316575	25.65737308709904
41	17.95254543324272	24.771732525833077	26.541424200121526	30.734297840802682
42	23.23201130597242	26.819759293958008	23.236915889123726	26.71131351094585
43	23.19018611076547	27.229155748670998	24.732041732553082	24.848616408010457
44	21.261595024936174	31.359495954173028	26.182299722709402	21.1966092981814
45	18.55971466225133	35.101329596162074	23.746720060017566	22.592235681569033
46	23.925964409074933	30.005195225115827	25.93298341251813	20.13585695329111
47	23.503625304379337	26.627345230883254	26.842738174278004	23.026291290459405
48	24.29626043699836	23.212256734946337	29.33222283882722	23.159259989228083
49	21.281485834383126	23.066299973388098	30.79206293125137	24.86015126097741
50	19.270833806383404	30.67571531982878	27.707897559606582	22.345553314181238
51	20.339215502842386	32.80235165679544	23.63968207402106	23.218750766341117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1616.0
1	2576.5
2	3537.0
3	9019.0
4	14501.0
5	10624.0
6	6747.0
7	7109.0
8	7471.0
9	7902.5
10	8334.0
11	8291.0
12	8248.0
13	8040.0
14	7832.0
15	7103.5
16	6375.0
17	6128.5
18	5882.0
19	6032.5
20	6183.0
21	6262.0
22	6341.0
23	6563.0
24	6785.0
25	9104.0
26	12594.5
27	13766.0
28	18414.5
29	23063.0
30	27073.0
31	31083.0
32	32332.5
33	33582.0
34	38543.0
35	43504.0
36	48345.5
37	53187.0
38	64661.5
39	76136.0
40	113381.5
41	150627.0
42	179121.5
43	207616.0
44	214862.5
45	222109.0
46	212752.5
47	203396.0
48	195555.0
49	187714.0
50	182591.0
51	177468.0
52	160864.0
53	144260.0
54	129601.0
55	114942.0
56	109085.0
57	103228.0
58	95615.0
59	88002.0
60	76543.5
61	65085.0
62	59850.5
63	54616.0
64	46340.0
65	38064.0
66	31220.5
67	24377.0
68	19878.5
69	15380.0
70	13290.5
71	11201.0
72	9685.5
73	8170.0
74	6570.5
75	3828.0
76	2685.0
77	2073.5
78	1462.0
79	985.0
80	508.0
81	448.5
82	389.0
83	229.0
84	69.0
85	49.5
86	30.0
87	26.0
88	22.0
89	17.0
90	12.0
91	11.0
92	10.0
93	7.5
94	5.0
95	6.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2202022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.69845221081983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25295310292249	14.380515074800584
2	7.854983663842901	2.780421059825881
3	2.301684685795937	1.2220876924780577
4	1.0996416447476633	0.7784782039437534
5	0.6569762173948068	0.5813731093603584
6	0.44778249659999475	0.47550342701499604
7	0.3328218558177656	0.4123302196934965
8	0.26148239178979804	0.3702266892050086
9	0.21507775890788167	0.3425889093877236
>10	2.451322229200049	10.487164644239568
>50	1.3376974962368804	17.572078631425704
>100	1.7745740094310607	48.20151183193736
>500	0.009361762065214125	1.0883446260857068
>1k	0.003380636301327323	0.9358649988085913
>5k	2.600489462559479E-4	0.3715108817932011
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8072	0.3665721777529925	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4478	0.20335854955127608	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2923	0.13274163473389458	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.082561391303084E-5	0.0	0.0	0.031516488027821705	0.0
2	9.082561391303084E-5	0.0	0.0	0.1263838417599824	0.0
3	9.082561391303084E-5	0.0	0.0	0.1813787509843226	0.0
4	9.082561391303084E-5	0.0	0.0	0.2897791211895249	0.0
5	9.082561391303084E-5	0.0	0.0	0.5506302843477495	0.0
6	9.082561391303084E-5	0.0	0.0	0.8605272790190107	0.0
7	9.082561391303084E-5	0.0	0.0	1.0840945276659362	0.0
8	9.082561391303084E-5	0.0	0.0	1.6927169664971558	0.0
9	9.082561391303084E-5	0.0	0.0	1.9935768125840705	0.0
10	9.082561391303084E-5	0.0	0.0	2.5196841811753017	0.0
11	9.082561391303084E-5	0.0	0.0	2.8432050179335175	0.0
12	9.082561391303084E-5	0.0	0.0	3.1928382186917297	0.0
13	9.082561391303084E-5	0.0	0.0	3.3130913315125827	0.0
14	9.082561391303084E-5	0.0	0.0	3.349694053919534	0.0
15	9.082561391303084E-5	0.0	0.0	3.4145889550603945	0.0
16	1.3623842086954625E-4	0.0	0.0	3.543697565237768	0.0
17	1.3623842086954625E-4	0.0	0.0	3.6901992804794865	0.0
18	1.3623842086954625E-4	0.0	0.0	3.96540089063597	0.0
19	1.3623842086954625E-4	0.0	0.0	4.072075574176825	0.0
20	1.3623842086954625E-4	0.0	0.0	4.182701171922896	0.0
21	1.3623842086954625E-4	0.0	0.0	4.3237533503298335	0.0
22	1.3623842086954625E-4	0.0	0.0	4.4584023229559016	0.0
23	1.3623842086954625E-4	0.0	0.0	4.615212745376749	0.0
24	1.3623842086954625E-4	0.0	0.0	4.737100719248037	0.0
25	1.3623842086954625E-4	0.0	0.0	4.826927251408024	0.0
26	1.8165122782606169E-4	0.0	0.0	4.923429466190619	0.0
27	1.8165122782606169E-4	0.0	0.0	5.013301411157563	0.0
28	1.8165122782606169E-4	0.0	0.0	5.118931600138418	0.0
29	1.8165122782606169E-4	0.0	0.0	5.225470045258404	0.0
30	1.8165122782606169E-4	0.0	0.0	5.3759680875122955	4.541280695651542E-5
31	1.8165122782606169E-4	0.0	0.0	5.510389996103581	4.541280695651542E-5
32	1.8165122782606169E-4	0.0	0.0	5.627963753314	4.541280695651542E-5
33	1.8165122782606169E-4	0.0	0.0	5.746173289821809	4.541280695651542E-5
34	2.270640347825771E-4	0.0	0.0	5.874736946315704	4.541280695651542E-5
35	2.270640347825771E-4	0.0	0.0	6.0436725881939415	4.541280695651542E-5
36	2.270640347825771E-4	0.0	0.0	6.178594037661749	4.541280695651542E-5
37	2.724768417390925E-4	0.0	0.0	6.306158612402601	4.541280695651542E-5
38	2.724768417390925E-4	0.0	0.0	6.436357129946931	4.541280695651542E-5
39	2.724768417390925E-4	0.0	0.0	6.565919868193869	4.541280695651542E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	45	3.8562575E-10	45.0	1
AAATCGT	20	7.034698E-4	45.0	36
TCCGATT	25	3.8919745E-5	45.0	29
ATTCGCA	20	7.034698E-4	45.0	17
ACGATAT	25	3.8919745E-5	45.0	24
ACCTAGT	40	6.8193913E-9	45.0	35
TCGTGAC	35	1.2126111E-7	45.0	17
CGGATAT	35	1.2126111E-7	45.0	12
TCGCTTA	20	7.034698E-4	45.0	41
TCGCTGA	40	6.8193913E-9	45.0	42
ACGTCCA	20	7.034698E-4	45.0	21
TCGACCC	20	7.034698E-4	45.0	17
CAATACG	25	3.8919745E-5	45.0	1
TACGTCA	35	1.2126111E-7	45.0	32
AGTCGAT	20	7.034698E-4	45.0	30
GTACGAT	20	7.034698E-4	45.0	27
ATCGTTC	30	2.166411E-6	44.999996	32
CGTAAAT	30	2.166411E-6	44.999996	18
ATACCGG	60	0.0	44.999996	2
TACCGGG	250	0.0	43.2	3
>>END_MODULE