FastQCFastQC Report
Thu 26 May 2016
SRR1546830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546830_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1369288
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501893.6653355612551923No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT28790.21025525674657194No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT26470.19331214470586172No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21730.15869561407096244No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC15310.1118099333376178No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC14610.10669778746326558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG302.165807E-645.0000041
CGGCTAT351.2121563E-745.00000440
CCGGATA406.8175723E-945.020
CACGACG3250.045.026
CTAACCG207.03376E-445.01
CGCGTCA406.8175723E-945.039
TCGGTAC406.8175723E-945.036
CGTTTTT206600.044.42281
CGACGGT3300.044.3181828
TCACGAC3400.043.01470625
ACGGTCT3400.043.01470630
GCCGACC900.042.514
GACGGTC3450.042.391329
CGGTCTA3450.042.391331
CGCGGGT850.042.352944
CGTTTTC4000.041.6251
GTTAGCG650.041.538461
TCGTTAG603.6379788E-1241.2500041
GCGATAT1550.040.645169
CTCACGA3600.040.62524