FastQCFastQC Report
Thu 26 May 2016
SRR1546829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546829_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1776568
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.44084999842392747No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27350.1539485119623904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25860.1455615546379311No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18450.10385192123239864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC453.8562575E-1045.00000433
GGTACGA253.891669E-545.08
CCCGTCA406.8193913E-945.044
TATTACC207.034327E-445.037
GCGATCG207.034327E-445.09
AATTACG351.2124292E-745.01
CACGTTA207.034327E-445.045
CGGTAAT253.891669E-545.06
ACTAACG302.1661745E-644.9999961
TAGCCGT850.042.3529444
CGTTTTT54250.041.350231
GTTCACG556.184564E-1140.9090921
TACGCGG1650.040.909092
ACATACG5750.040.69565217
TTTAGCG1000.040.51
CGCGATA501.0822987E-940.511
TCGTAAT501.0822987E-940.532
TATTACG900.040.0000041
CGGTATA403.459645E-739.37535
CATACGA5900.039.27966318