FastQCFastQC Report
Thu 26 May 2016
SRR1546828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546828_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4686462
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495251.056767343893965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG800.045.01
CGTTTTT312450.043.178111
CGATTCG2050.038.41463510
TAGGGTA31000.038.395165
TATTACG1000.038.251
GCGATAT4650.037.2580649
GTTTTTT380650.037.215292
GGCGATT22350.037.147658
GGGCGAT76000.037.0953947
ATTAGCG3600.036.8750041
TAGGGCG16550.036.706955
ATAGGGC22600.036.637174
AGGGCGA41000.036.49396
TAGGGAC29700.036.4393965
GCGTTAG4700.036.382981
CGTTAGG8850.036.3559342
ACGGGTA10150.036.354685
CGAGGGT11250.035.84
GCGCGAC11200.035.7589269
GTACGGG16300.035.7515343
Produced by FastQC (version 0.11.5)