##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546828_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4686462
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95137739300991	33.0	31.0	34.0	30.0	34.0
2	32.35613304876899	34.0	31.0	34.0	31.0	34.0
3	32.395917645336716	34.0	31.0	34.0	30.0	34.0
4	36.018900185257024	37.0	35.0	37.0	35.0	37.0
5	35.98277250514354	37.0	35.0	37.0	35.0	37.0
6	36.03451025528426	37.0	35.0	37.0	35.0	37.0
7	36.26477479172988	37.0	36.0	37.0	35.0	37.0
8	36.13813554873591	37.0	36.0	37.0	35.0	37.0
9	37.94122026381522	39.0	39.0	39.0	35.0	39.0
10	37.60177144293499	39.0	37.0	39.0	35.0	39.0
11	37.47869949654985	39.0	37.0	39.0	35.0	39.0
12	37.07639771751057	39.0	35.0	39.0	34.0	39.0
13	36.9861255676457	39.0	35.0	39.0	33.0	39.0
14	38.198676101502585	40.0	37.0	41.0	33.0	41.0
15	38.29983962315282	40.0	37.0	41.0	33.0	41.0
16	38.345406620175304	40.0	36.0	41.0	34.0	41.0
17	38.2866857770318	40.0	36.0	41.0	34.0	41.0
18	38.13852966267517	40.0	36.0	41.0	34.0	41.0
19	38.04252397650936	40.0	36.0	41.0	34.0	41.0
20	37.891281312000395	40.0	35.0	41.0	34.0	41.0
21	37.775266715061385	40.0	35.0	41.0	34.0	41.0
22	37.738118862374215	40.0	35.0	41.0	33.0	41.0
23	37.64428944478799	40.0	35.0	41.0	33.0	41.0
24	37.622739926195926	40.0	35.0	41.0	33.0	41.0
25	37.53834726495168	39.0	35.0	41.0	33.0	41.0
26	37.49746866612809	39.0	35.0	41.0	33.0	41.0
27	37.43407713537419	39.0	35.0	41.0	33.0	41.0
28	37.3811160743435	39.0	35.0	41.0	33.0	41.0
29	37.276182971290496	39.0	35.0	41.0	33.0	41.0
30	37.194663692994844	39.0	35.0	41.0	33.0	41.0
31	36.98188462853214	39.0	35.0	41.0	32.0	41.0
32	36.756660568249565	39.0	35.0	41.0	31.0	41.0
33	36.54283679244599	39.0	35.0	41.0	31.0	41.0
34	36.30888013174971	39.0	35.0	41.0	30.0	41.0
35	36.138090952193785	39.0	35.0	41.0	30.0	41.0
36	36.027884574760236	39.0	35.0	41.0	29.0	41.0
37	35.77563138248	39.0	35.0	41.0	27.0	41.0
38	35.83748443922089	39.0	35.0	41.0	28.0	41.0
39	35.73422423994903	39.0	35.0	41.0	27.0	41.0
40	35.619502729351055	39.0	35.0	41.0	27.0	41.0
41	35.551039568868795	39.0	35.0	41.0	26.0	41.0
42	35.54065604287413	38.0	35.0	41.0	27.0	41.0
43	35.44548766212123	38.0	35.0	41.0	26.0	41.0
44	35.31105704047104	38.0	35.0	41.0	26.0	41.0
45	35.26676904667103	38.0	35.0	40.0	26.0	41.0
46	35.11150010391634	38.0	35.0	40.0	25.0	41.0
47	35.092227569539666	38.0	35.0	40.0	25.0	41.0
48	34.94864270744114	38.0	35.0	40.0	24.0	41.0
49	34.91436887784431	37.0	34.0	40.0	24.0	41.0
50	34.796342528756234	37.0	34.0	40.0	24.0	41.0
51	34.26301056105864	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	4.0
12	13.0
13	33.0
14	55.0
15	91.0
16	249.0
17	601.0
18	1444.0
19	2905.0
20	5187.0
21	8649.0
22	13310.0
23	19979.0
24	30269.0
25	48634.0
26	71481.0
27	84032.0
28	83199.0
29	79908.0
30	83143.0
31	95465.0
32	115468.0
33	152088.0
34	273585.0
35	525954.0
36	292173.0
37	388697.0
38	668445.0
39	1640747.0
40	644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.01076590400178	22.356993399285006	27.973533125842053	16.658707570871158
2	35.16847037274601	24.12960992748901	28.055983383627137	12.645936316137846
3	28.91737946450862	24.04775713534005	33.61695880602467	13.417904594126655
4	25.869643240465834	26.46907197796547	33.11679471635532	14.544490065213376
5	23.269195397295444	30.42273681083939	31.776978027347706	14.531089764517455
6	20.977018484306498	38.99210107752927	29.2882775961909	10.742602841973326
7	83.26957521473555	4.264176259190836	10.382096344747914	2.0841521813256993
8	83.80780640064937	3.5635837866603848	10.182030708880173	2.4465791038100813
9	78.22359383261829	5.149983078919663	12.848925266010905	3.777497822451137
10	38.51257089036463	23.654710099004323	24.83784142493847	12.994877585692578
11	34.69610977321484	24.174633230782625	25.50030278704916	15.628954208953363
12	30.3763692098645	22.940525283252057	29.626336455944802	17.056769050938637
13	23.115476024344165	29.00584705477181	30.213602500137632	17.665074420746397
14	18.498923068190887	31.620804777676636	32.17156140389061	17.708710750241867
15	18.020822530941253	24.23514796449859	40.56510006909264	17.178929435467523
16	20.32563157452253	22.365549960716635	38.73954381791637	18.569274646844462
17	21.072975733079666	21.349218237553192	31.724934502829637	25.852871526537502
18	23.344027968219947	23.48315210920306	33.30149695015131	19.87132297242568
19	26.21288724841896	25.27815226070328	29.46403918350346	19.044921307374306
20	27.70215569869125	24.74399664395017	28.47403862444633	19.079809032912248
21	24.631886484943223	25.76619633318269	30.18998126945231	19.41193591242178
22	23.084791896317522	23.70600679147724	31.047813894575484	22.16138741762976
23	21.379496942469608	26.43776904624427	30.298762691343704	21.88397131994242
24	21.04327315574094	25.190538192777407	34.372944878247175	19.393243773234477
25	20.87380629566611	25.48878023549535	32.71277991798504	20.9246335508535
26	19.766702471928717	29.23687421342582	29.891013732747645	21.105409581897817
27	20.140929340726544	27.13595031817179	32.41193463213827	20.311185708963393
28	19.166548240442363	26.17277169856493	35.81654988347287	18.844130177519844
29	20.111696200673343	25.214756889098854	34.36671416518474	20.306832745043064
30	21.471890735484468	25.979619593629483	32.80461892148064	19.743870749405414
31	25.36508351075929	25.348653205765885	29.133320615850504	20.15294266762432
32	25.92192148362667	26.32800607366495	29.059405581438618	18.690666861269758
33	24.095661076522116	26.994927089988142	28.833926317977188	20.075485515512558
34	21.46064557869028	27.703137249379168	30.403233825431634	20.432983346498915
35	21.661137975726678	27.34706906830782	30.338686198671834	20.653106757293667
36	24.68631987200579	27.455807814082352	28.328171656998393	19.52970065691347
37	22.259691852830557	29.28612245228917	28.731973074784346	19.722212620095927
38	22.294238169433573	29.300440289497708	27.23711405320261	21.168207487866113
39	22.58300611420726	27.775558619700746	28.020882277504867	21.62055298858713
40	23.09913107158449	25.6735891595835	29.09777141050114	22.129508358330867
41	20.677602848374743	26.038448620729245	29.070864118817134	24.213084412078878
42	22.886134572306357	27.01558233055128	27.50501337682883	22.593269720313533
43	22.88015991594512	27.093828990825063	27.688413989060408	22.33759710416941
44	22.070252570062447	28.483320679864683	28.700008663251726	20.746418086821144
45	20.59216526240904	29.943740075135572	27.733970743814844	21.730123918640544
46	22.405388115811032	28.606611981490516	28.450865492988104	20.537134409710355
47	22.385458369234616	27.59113378066439	29.02402708055672	20.999380769544274
48	22.933249005326406	25.9117218916957	29.66461693277359	21.490412170204305
49	21.882136246917185	25.787449039381947	29.907806784734415	22.422607928966457
50	20.29206680860743	28.516757417429183	29.5012527574106	21.689923016552786
51	20.510973950071502	29.482283223463668	27.78112785295176	22.225614973513068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3316.0
1	5517.5
2	7719.0
3	46533.5
4	85348.0
5	58575.5
6	31803.0
7	32197.5
8	32592.0
9	33203.0
10	33814.0
11	33442.5
12	33071.0
13	31500.5
14	29930.0
15	28480.5
16	27031.0
17	25791.0
18	24551.0
19	22847.0
20	21143.0
21	22272.5
22	23402.0
23	25324.5
24	27247.0
25	27828.0
26	34430.5
27	40452.0
28	51190.5
29	61929.0
30	68233.5
31	74538.0
32	86900.5
33	99263.0
34	109194.5
35	119126.0
36	136085.0
37	153044.0
38	176812.0
39	200580.0
40	239840.5
41	279101.0
42	308766.5
43	338432.0
44	358184.5
45	377937.0
46	375556.0
47	373175.0
48	362920.5
49	352666.0
50	337874.5
51	323083.0
52	310558.5
53	298034.0
54	271414.0
55	244794.0
56	230400.5
57	216007.0
58	203198.0
59	190389.0
60	175004.0
61	159619.0
62	139405.0
63	119191.0
64	102279.5
65	85368.0
66	72531.5
67	59695.0
68	50324.5
69	40954.0
70	33740.0
71	26526.0
72	22845.0
73	19164.0
74	15764.5
75	8914.0
76	5463.0
77	4286.0
78	3109.0
79	2440.5
80	1772.0
81	1232.5
82	693.0
83	541.0
84	389.0
85	240.0
86	91.0
87	86.0
88	81.0
89	53.0
90	25.0
91	17.0
92	9.0
93	8.5
94	8.0
95	5.5
96	3.0
97	6.5
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4686462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.38327673366374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37816686769035	15.773755284747079
2	7.082539822022707	2.745656586949193
3	2.245459024498106	1.3057306099784822
4	1.1000135661680646	0.8528746945527971
5	0.713788346884686	0.6917778528465116
6	0.5089267017706305	0.5918800258542533
7	0.3879354381993247	0.5263621967388834
8	0.3060618879452121	0.4745985817335703
9	0.2660270211139802	0.46408278319960355
>10	3.1465085381089137	14.823608560683818
>50	1.542852818312042	21.915486374617874
>100	1.3140473442105343	37.24555172210275
>500	0.0040619769223094095	0.5010789889176592
>1k	0.0034978134608771635	1.004410657153395
>5k	0.0	0.0
>10k+	1.1283269228636011E-4	1.0831450799242657
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49525	1.056767343893965	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.535223373197094E-5	0.0	0.0	0.028657012475509242	0.0
2	8.535223373197094E-5	0.0	0.0	0.1051539519577882	0.0
3	8.535223373197094E-5	0.0	0.0	0.17768201257153052	0.0
4	8.535223373197094E-5	0.0	0.0	0.26363171193962526	0.0
5	8.535223373197094E-5	0.0	0.0	0.47654285898402676	0.0
6	8.535223373197094E-5	0.0	0.0	0.8067279751761564	0.0
7	8.535223373197094E-5	0.0	0.0	1.0354719615778385	0.0
8	8.535223373197094E-5	0.0	0.0	1.5525998077014174	0.0
9	8.535223373197094E-5	0.0	0.0	1.8477691700050058	0.0
10	8.535223373197094E-5	0.0	0.0	2.3089699649757107	0.0
11	8.535223373197094E-5	0.0	0.0	2.5708946322406967	0.0
12	8.535223373197094E-5	0.0	0.0	2.810990465728731	0.0
13	1.0669029216496367E-4	0.0	0.0	2.9132211036811992	0.0
14	1.0669029216496367E-4	0.0	0.0	2.9597807471819895	0.0
15	1.0669029216496367E-4	0.0	0.0	3.016454630380018	0.0
16	1.4936640903094915E-4	0.0	0.0	3.125876194024405	0.0
17	1.4936640903094915E-4	0.0	0.0	3.247609817384628	0.0
18	1.4936640903094915E-4	0.0	0.0	3.4371984665617688	0.0
19	1.4936640903094915E-4	0.0	0.0	3.521846544365451	0.0
20	1.4936640903094915E-4	0.0	0.0	3.624909366596806	0.0
21	1.4936640903094915E-4	0.0	0.0	3.7410950947644515	0.0
22	1.4936640903094915E-4	0.0	0.0	3.8698276012906967	0.0
23	1.4936640903094915E-4	0.0	0.0	4.004449411944448	0.0
24	1.4936640903094915E-4	0.0	0.0	4.116004781432133	0.0
25	1.4936640903094915E-4	0.0	0.0	4.210062943004766	0.0
26	1.4936640903094915E-4	0.0	0.0	4.304761246330387	0.0
27	1.4936640903094915E-4	0.0	0.0	4.407909420795474	0.0
28	1.707044674639419E-4	0.0	0.0	4.50777153426188	0.0
29	1.707044674639419E-4	0.0	0.0	4.625536278753567	0.0
30	1.707044674639419E-4	0.0	0.0	4.782925797755322	0.0
31	1.707044674639419E-4	0.0	0.0	4.90497949199204	0.0
32	1.707044674639419E-4	0.0	0.0	5.033050518706863	0.0
33	1.707044674639419E-4	0.0	0.0	5.152628998165354	0.0
34	1.707044674639419E-4	0.0	0.0	5.278288824277248	0.0
35	1.707044674639419E-4	0.0	0.0	5.421744591122258	0.0
36	1.707044674639419E-4	0.0	0.0	5.5492181522009565	0.0
37	1.707044674639419E-4	0.0	0.0	5.683861300913141	0.0
38	1.707044674639419E-4	0.0	0.0	5.8305177765230995	0.0
39	1.707044674639419E-4	0.0	0.0	6.030220665397479	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	80	0.0	45.0	1
CGTTTTT	31245	0.0	43.17811	1
CGATTCG	205	0.0	38.414635	10
TAGGGTA	3100	0.0	38.39516	5
TATTACG	100	0.0	38.25	1
GCGATAT	465	0.0	37.258064	9
GTTTTTT	38065	0.0	37.21529	2
GGCGATT	2235	0.0	37.14765	8
GGGCGAT	7600	0.0	37.095394	7
ATTAGCG	360	0.0	36.875004	1
TAGGGCG	1655	0.0	36.70695	5
ATAGGGC	2260	0.0	36.63717	4
AGGGCGA	4100	0.0	36.4939	6
TAGGGAC	2970	0.0	36.439396	5
GCGTTAG	470	0.0	36.38298	1
CGTTAGG	885	0.0	36.355934	2
ACGGGTA	1015	0.0	36.35468	5
CGAGGGT	1125	0.0	35.8	4
GCGCGAC	1120	0.0	35.758926	9
GTACGGG	1630	0.0	35.751534	3
>>END_MODULE