FastQCFastQC Report
Thu 26 May 2016
SRR1546827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546827_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences686946
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.5533186014621236No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11420.1662430525834636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG207.0313015E-445.0000041
CCGGTAC406.8084773E-945.00000417
TGTCGTT207.0313015E-445.00000420
ATCGACG207.0313015E-445.0000041
TAGCCGT351.210974E-745.044
AACACGT600.044.99999641
CGTTTTT25200.042.321431
CGGTACA451.9263098E-840.018
AATGCGG800.039.3750042
CCGACAC403.455516E-739.37500411
ACACGAC700.038.57142626
CGGGCTA356.2446197E-638.5714266
AAACACG700.038.57142640
TGACGGG2700.038.3333323
AGGGCGA5000.038.2500046
GGACCGA3150.037.8571438
TAGGGCG2700.037.55
CGTTCGG301.1394437E-437.49999645
TCGATAC301.1394437E-437.49999624
GGACGTA301.1394437E-437.4999967