##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546827_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	686946
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916111310059307	33.0	31.0	34.0	30.0	34.0
2	32.299611614304474	33.0	31.0	34.0	31.0	34.0
3	32.35924366689667	34.0	31.0	34.0	30.0	34.0
4	35.97367333094596	37.0	35.0	37.0	35.0	37.0
5	35.93724834266449	37.0	35.0	37.0	35.0	37.0
6	35.98407880677666	37.0	35.0	37.0	35.0	37.0
7	36.19575483371327	37.0	35.0	37.0	35.0	37.0
8	36.05765518687058	37.0	35.0	37.0	35.0	37.0
9	37.81923761110772	39.0	38.0	39.0	35.0	39.0
10	37.605973395288714	39.0	37.0	39.0	35.0	39.0
11	37.40936842197203	39.0	37.0	39.0	35.0	39.0
12	36.846756513612426	39.0	35.0	39.0	33.0	39.0
13	36.69211262602883	39.0	35.0	39.0	33.0	39.0
14	37.848788696637	40.0	35.0	41.0	33.0	41.0
15	37.97358889927301	40.0	35.0	41.0	33.0	41.0
16	38.04445182008484	40.0	35.0	41.0	34.0	41.0
17	37.993900539489275	40.0	35.0	41.0	34.0	41.0
18	37.898377747304735	40.0	36.0	41.0	34.0	41.0
19	37.855329239852914	40.0	36.0	41.0	34.0	41.0
20	37.72499585120228	40.0	35.0	41.0	34.0	41.0
21	37.56869972312234	39.0	35.0	41.0	33.0	41.0
22	37.54048789861212	39.0	35.0	41.0	33.0	41.0
23	37.44452547944089	39.0	35.0	41.0	33.0	41.0
24	37.436316682825144	39.0	35.0	41.0	33.0	41.0
25	37.319064380606335	39.0	35.0	41.0	33.0	41.0
26	37.27101984726602	39.0	35.0	41.0	33.0	41.0
27	37.19108488876855	39.0	35.0	41.0	33.0	41.0
28	37.111272210624996	39.0	35.0	41.0	33.0	41.0
29	37.025943815088816	39.0	35.0	41.0	33.0	41.0
30	37.008577093395985	39.0	35.0	41.0	32.0	41.0
31	36.828641552611124	39.0	35.0	41.0	32.0	41.0
32	36.68169696016863	39.0	35.0	41.0	31.0	41.0
33	36.57755922590713	39.0	35.0	41.0	31.0	41.0
34	36.409502348073936	39.0	35.0	41.0	31.0	41.0
35	36.28716958829369	39.0	35.0	41.0	30.0	41.0
36	36.194061833098964	39.0	35.0	41.0	30.0	41.0
37	35.92721698648802	38.0	35.0	41.0	29.0	41.0
38	36.01824597566621	38.0	35.0	41.0	30.0	41.0
39	35.921673610443904	38.0	35.0	41.0	30.0	41.0
40	35.81216864207667	38.0	35.0	41.0	29.0	41.0
41	35.74686074305695	38.0	35.0	41.0	29.0	41.0
42	35.74547344332742	38.0	35.0	41.0	29.0	41.0
43	35.64617306163803	38.0	35.0	40.0	29.0	41.0
44	35.50053133725213	38.0	35.0	40.0	28.0	41.0
45	35.451828819150265	38.0	35.0	40.0	28.0	41.0
46	35.275165733551106	37.0	35.0	40.0	27.0	41.0
47	35.259889423622816	37.0	35.0	40.0	28.0	41.0
48	35.13150669776081	37.0	35.0	40.0	27.0	41.0
49	35.100577046812994	37.0	35.0	40.0	27.0	41.0
50	34.97500968052802	37.0	34.0	40.0	27.0	41.0
51	34.41323335458682	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	4.0
13	8.0
14	8.0
15	31.0
16	45.0
17	121.0
18	253.0
19	463.0
20	802.0
21	1265.0
22	2030.0
23	2931.0
24	4316.0
25	6380.0
26	8519.0
27	9982.0
28	10058.0
29	10788.0
30	12192.0
31	14625.0
32	18116.0
33	24014.0
34	46151.0
35	92662.0
36	43075.0
37	58349.0
38	97750.0
39	221919.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.98835134057117	22.24643567325525	26.97679293568927	13.788420050484318
2	34.191479388481774	24.871532842465054	27.7641910717873	13.17279669726587
3	30.661362028456384	24.892204045150564	30.317666890847317	14.128767035545733
4	26.977375223088863	27.08990226305998	29.607567407045092	16.325155106806065
5	23.76678807358948	31.31570749374769	28.982481883583276	15.93502254907955
6	21.589906630215474	39.808078073094535	27.794324444716178	10.807690851973808
7	83.22939503250619	5.545705193712461	8.279835678495836	2.94506409528551
8	83.64543937951456	4.859188349593709	8.25668975436206	3.2386825165296833
9	78.0553347715832	6.7024191130016035	10.569535305540755	4.672710809874429
10	44.51921985134203	25.662133559260848	16.6572044964233	13.161442092973829
11	38.00051241291164	24.290992305072017	22.818824187054005	14.88967109496234
12	33.76014999723414	23.03601738710175	27.139251120175384	16.064581495488728
13	23.395871000049496	32.20355020627531	27.91034520908485	16.490233584590346
14	18.511789864123234	35.219071076911426	29.258922826539496	17.010216232425837
15	16.772642973392376	24.81010152180812	41.71623388155692	16.701021623242585
16	19.345188704789024	21.986298777487605	39.66905695644199	18.99945556128138
17	20.839192600291724	21.336757183242934	28.68653431274074	29.137515903724605
18	23.320464781802354	23.81686478995438	32.02566140570001	20.837009022543256
19	28.04252444879219	24.886381171154646	26.919874342379167	20.151220037673994
20	29.684720487491013	24.00523476372233	25.619335435390845	20.690709313395814
21	25.268507277136777	26.747226128400193	27.93334556136872	20.050921033094305
22	24.071178811726103	24.734695303560976	27.91806051712944	23.276065367583477
23	22.134927636233414	28.535285160696766	26.3840243629048	22.94576284016502
24	21.90667097559342	25.580467751468095	32.8452309206255	19.667630352312994
25	20.406116346845312	26.269022601485414	31.54614773213615	21.77871331953312
26	19.783505544831762	30.72046419951495	27.756039048193017	21.739991207460267
27	20.97544785179621	28.912316251932467	29.412646700031736	20.699589196239586
28	19.222326063475148	26.70748501337805	34.837381686479	19.2328072366678
29	19.720181790126155	25.37288229351361	34.05667985547627	20.85025606088397
30	21.332390027745994	26.867905191965598	30.76864848183118	21.03105629845723
31	26.85043656997784	25.710754557126762	26.120539314589504	21.318269558305893
32	27.462129483249047	27.691696290538122	26.270187176284598	18.575987049928234
33	25.80610411880992	26.63076864848183	26.920456629778762	20.64267060292949
34	21.51843085191558	28.184893717992388	28.879562585705425	21.417112844386605
35	21.903613966745567	26.83704395978723	29.649783243515504	21.6095588299517
36	27.07709194026896	25.90145368049308	27.16705534350589	19.85439903573207
37	22.620846471192788	28.88989818704818	28.344731609180347	20.14452373257869
38	22.440337377319324	30.1052775618462	25.600265523054215	21.85411953778026
39	22.184713208898515	29.027463585201748	26.828891936192946	21.958931269706788
40	23.099049998107564	26.355346708474904	27.317285492600586	23.22831780081695
41	20.331874703397357	25.370698715765137	28.280388851525444	26.017037729312058
42	23.094973986310425	27.373476226661193	26.17614776125052	23.355402025777863
43	23.383788536507964	27.755893476343118	26.196964535785927	22.663353451362987
44	22.293164237072492	29.176820303197047	27.36794449636507	21.162070963365387
45	20.36215364817613	31.33958127713095	25.82619303409584	22.472072040597077
46	23.0753217865742	29.030666165899504	27.35018473067752	20.543827316848777
47	22.849539847382474	27.693734296436688	27.823729958395564	21.63299589778527
48	23.465017628751024	25.329065166694324	28.988304757579197	22.217612446975455
49	21.862562705074343	25.32571701414667	29.866394156163658	22.945326124615324
50	20.64965805172459	29.56753514832316	27.595327725905676	22.187479074046575
51	20.65300620427224	30.862542325015358	25.634183764080436	22.850267706631964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	580.0
1	846.5
2	1113.0
3	3889.0
4	6665.0
5	4739.0
6	2813.0
7	2883.0
8	2953.0
9	3058.5
10	3164.0
11	3170.0
12	3176.0
13	3175.5
14	3175.0
15	3092.5
16	3010.0
17	2987.0
18	2964.0
19	2795.0
20	2626.0
21	2792.0
22	2958.0
23	3411.5
24	3865.0
25	4287.5
26	5282.5
27	5855.0
28	6697.5
29	7540.0
30	9135.0
31	10730.0
32	11967.0
33	13204.0
34	14946.5
35	16689.0
36	18351.5
37	20014.0
38	23655.5
39	27297.0
40	34243.5
41	41190.0
42	48607.0
43	56024.0
44	57330.0
45	58636.0
46	58090.5
47	57545.0
48	55445.5
49	53346.0
50	51810.5
51	50275.0
52	46962.5
53	43650.0
54	40630.0
55	37610.0
56	34240.0
57	30870.0
58	28915.5
59	26961.0
60	25992.0
61	25023.0
62	21591.5
63	18160.0
64	16385.0
65	14610.0
66	12271.0
67	9932.0
68	8267.0
69	6602.0
70	5513.0
71	4424.0
72	3691.0
73	2958.0
74	2405.0
75	1485.5
76	1119.0
77	799.0
78	479.0
79	396.5
80	314.0
81	255.0
82	196.0
83	116.0
84	36.0
85	25.5
86	15.0
87	12.0
88	9.0
89	6.0
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.13369826571661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76917721451153	20.55897992380166
2	5.353209665388144	2.905047516275194
3	2.2859370632080878	1.8607777958251988
4	1.5549706756393058	1.687684204993377
5	1.1957689138221421	1.6222816451586848
6	0.9887107534058013	1.6096427552989414
7	0.9072581109667301	1.7232087482466703
8	0.7448401080926544	1.6168213399351865
9	0.6697237983939809	1.6354875120292711
>10	10.304468930959201	59.94480810337307
>50	0.20805503595965189	3.366305682579133
>100	0.016796109673824396	0.742269079890845
>500	0.0	0.0
>1k	0.0010836199789564128	0.726685692592681
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3801	0.5533186014621236	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1142	0.1662430525834636	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017177478287958587	0.0
2	0.0	0.0	0.0	0.07074791905040571	0.0
3	0.0	0.0	0.0	0.11602076436866944	0.0
4	0.0	0.0	0.0	0.1831293871716263	0.0
5	0.0	0.0	0.0	0.35024587085447767	0.0
6	0.0	0.0	0.0	0.6100916229223258	0.0
7	0.0	0.0	0.0	0.799626171489462	0.0
8	0.0	0.0	0.0	1.298064185540057	0.0
9	0.0	0.0	0.0	1.5574732220582113	0.0
10	0.0	0.0	0.0	1.945422202036259	0.0
11	0.0	0.0	0.0	2.171786428627578	0.0
12	0.0	0.0	0.0	2.396549364870019	0.0
13	0.0	0.0	0.0	2.4854937651576687	0.0
14	0.0	0.0	0.0	2.5218867276321575	0.0
15	0.0	0.0	0.0	2.573710306195829	0.0
16	0.0	0.0	0.0	2.669205439728887	0.0
17	0.0	0.0	0.0	2.7772197523531688	0.0
18	0.0	0.0	0.0	2.947247673033979	0.0
19	0.0	0.0	0.0	3.017704448384589	0.0
20	0.0	0.0	0.0	3.104028555374076	0.0
21	0.0	0.0	0.0	3.2068022814020316	0.0
22	0.0	0.0	0.0	3.307829145231212	0.0
23	0.0	0.0	0.0	3.4196283259528406	0.0
24	0.0	0.0	0.0	3.5149778876360007	0.0
25	0.0	0.0	0.0	3.5948968332299773	0.0
26	0.0	0.0	0.0	3.678455075071403	0.0
27	0.0	0.0	0.0	3.7598297391643594	0.0
28	0.0	0.0	0.0	3.843679124705581	0.0
29	0.0	0.0	0.0	3.933351384242721	0.0
30	0.0	0.0	0.0	4.063201474351696	0.0
31	0.0	0.0	0.0	4.1674309188786305	0.0
32	0.0	0.0	0.0	4.275299659653015	0.0
33	0.0	0.0	0.0	4.375453092382807	0.0
34	0.0	0.0	0.0	4.472549516264743	0.0
35	0.0	0.0	0.0	4.599779313075555	0.0
36	0.0	0.0	0.0	4.726426822486775	0.0
37	0.0	0.0	0.0	4.837352572109016	0.0
38	1.4557184989795414E-4	0.0	0.0	4.952645477228195	0.0
39	1.4557184989795414E-4	0.0	0.0	5.098945186375639	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	20	7.0313015E-4	45.000004	1
CCGGTAC	40	6.8084773E-9	45.000004	17
TGTCGTT	20	7.0313015E-4	45.000004	20
ATCGACG	20	7.0313015E-4	45.000004	1
TAGCCGT	35	1.210974E-7	45.0	44
AACACGT	60	0.0	44.999996	41
CGTTTTT	2520	0.0	42.32143	1
CGGTACA	45	1.9263098E-8	40.0	18
AATGCGG	80	0.0	39.375004	2
CCGACAC	40	3.455516E-7	39.375004	11
ACACGAC	70	0.0	38.571426	26
CGGGCTA	35	6.2446197E-6	38.571426	6
AAACACG	70	0.0	38.571426	40
TGACGGG	270	0.0	38.333332	3
AGGGCGA	500	0.0	38.250004	6
GGACCGA	315	0.0	37.857143	8
TAGGGCG	270	0.0	37.5	5
CGTTCGG	30	1.1394437E-4	37.499996	45
TCGATAC	30	1.1394437E-4	37.499996	24
GGACGTA	30	1.1394437E-4	37.499996	7
>>END_MODULE