FastQCFastQC Report
Thu 26 May 2016
SRR1546826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546826_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4298882
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511021.188727673846363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT283200.043.3712961
CGGTCTA5200.040.24038331
CGTAAGG7000.039.5357132
TACGGGA15000.039.1499984
TAGGGAC37850.038.104365
ACGGGTC2800.036.9642835
AGGGCGA29500.036.8389856
ACGGGAT15000.036.755
TAGCGGG24450.036.6257673
GTTTTTT350250.036.603852
CGTTAGG6100.036.5163962
AGGGATT56150.036.22446
CGACGGT6100.036.1475428
CGTACCG250.002107749236.00000436
TCGCGAT250.002107749236.00000423
TCGTTAG2250.036.01
GTAGGGA49350.035.7446824
GTACGGG14150.035.6183743
GGGCGAT59950.035.617187
GCGCGAC20500.035.5609789
Produced by FastQC (version 0.11.5)