##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546826_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4298882
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95707767740543	33.0	31.0	34.0	30.0	34.0
2	32.35664737948146	34.0	31.0	34.0	31.0	34.0
3	32.383019817710746	34.0	31.0	34.0	30.0	34.0
4	36.00495942898642	37.0	35.0	37.0	35.0	37.0
5	35.96790863298876	37.0	35.0	37.0	35.0	37.0
6	36.02312461705159	37.0	35.0	37.0	35.0	37.0
7	36.24909104274088	37.0	36.0	37.0	35.0	37.0
8	36.1351130363662	37.0	36.0	37.0	35.0	37.0
9	37.946576807644405	39.0	39.0	39.0	35.0	39.0
10	37.6809821716437	39.0	38.0	39.0	35.0	39.0
11	37.4654442713245	39.0	37.0	39.0	35.0	39.0
12	37.02542870448642	39.0	35.0	39.0	34.0	39.0
13	36.91705913304901	39.0	35.0	39.0	33.0	39.0
14	38.117720142120675	40.0	36.0	41.0	33.0	41.0
15	38.234090863624544	40.0	36.0	41.0	33.0	41.0
16	38.30038368115245	40.0	36.0	41.0	34.0	41.0
17	38.23449748097296	40.0	36.0	41.0	34.0	41.0
18	38.08834738892577	40.0	36.0	41.0	34.0	41.0
19	38.00064947118809	40.0	36.0	41.0	34.0	41.0
20	37.83203260754773	40.0	35.0	41.0	34.0	41.0
21	37.7058912061322	40.0	35.0	41.0	33.0	41.0
22	37.67027822582709	40.0	35.0	41.0	33.0	41.0
23	37.59169756229643	39.0	35.0	41.0	33.0	41.0
24	37.57240766320174	39.0	35.0	41.0	33.0	41.0
25	37.46782675123439	39.0	35.0	41.0	33.0	41.0
26	37.43227820628712	39.0	35.0	41.0	33.0	41.0
27	37.36260218354447	39.0	35.0	41.0	33.0	41.0
28	37.29330067678062	39.0	35.0	41.0	33.0	41.0
29	37.16328687319168	39.0	35.0	41.0	33.0	41.0
30	37.10736396114152	39.0	35.0	41.0	33.0	41.0
31	36.90091516817628	39.0	35.0	41.0	32.0	41.0
32	36.70076545483221	39.0	35.0	41.0	31.0	41.0
33	36.48327355810185	39.0	35.0	41.0	31.0	41.0
34	36.238062128711604	39.0	35.0	41.0	30.0	41.0
35	36.070028905189766	39.0	35.0	41.0	30.0	41.0
36	35.97649435364823	39.0	35.0	41.0	29.0	41.0
37	35.72929496552825	39.0	35.0	41.0	27.0	41.0
38	35.80262891607632	39.0	35.0	41.0	29.0	41.0
39	35.70395093421964	39.0	35.0	41.0	28.0	41.0
40	35.59557764088431	38.0	35.0	41.0	27.0	41.0
41	35.50903002222438	38.0	35.0	41.0	27.0	41.0
42	35.49593824626961	38.0	35.0	41.0	27.0	41.0
43	35.38864267500248	38.0	35.0	41.0	26.0	41.0
44	35.253269803637316	38.0	35.0	40.0	26.0	41.0
45	35.208795914844835	38.0	35.0	40.0	26.0	41.0
46	35.04975107481434	38.0	35.0	40.0	25.0	41.0
47	35.027642070659304	38.0	35.0	40.0	25.0	41.0
48	34.89471820812946	37.0	34.0	40.0	24.0	41.0
49	34.85069955397706	37.0	34.0	40.0	24.0	41.0
50	34.73294521691919	37.0	34.0	40.0	24.0	41.0
51	34.19495347860211	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	13.0
13	30.0
14	41.0
15	109.0
16	229.0
17	595.0
18	1315.0
19	2675.0
20	4803.0
21	7723.0
22	12113.0
23	18055.0
24	27651.0
25	44819.0
26	65855.0
27	77428.0
28	76422.0
29	74105.0
30	78326.0
31	88738.0
32	108826.0
33	143268.0
34	263953.0
35	497039.0
36	269294.0
37	357124.0
38	603965.0
39	1473806.0
40	549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.24554663282221	22.98481326075012	27.765358528101025	17.004281578326644
2	34.77848426637437	23.891560642976476	28.313849973086025	13.016105117563125
3	28.639632350922867	24.12013169935811	34.15255408266614	13.087681867052876
4	25.87633249761217	26.265294092743186	33.02235325370643	14.836020155938218
5	22.564052700213683	30.77169831598076	32.08108526821625	14.58316371558931
6	20.794197188943546	38.67051945133642	29.848737415914183	10.686545943805854
7	83.27169715288765	4.257781441779514	10.371115094575753	2.0994063107570757
8	84.3084783439043	3.4224712378706834	9.880638733512574	2.3884116847124437
9	79.250651681065	5.581567486616288	11.807721170294974	3.3600596620237537
10	41.74050369375107	29.44842403210881	17.36167682667261	11.449395447467504
11	33.14454781499004	23.326506752220695	27.790481339101653	15.738464093687615
12	30.409092410538367	21.78405920423031	30.55347878820586	17.253369597025458
13	23.327855940218875	28.641400252437727	31.31546760297212	16.71527620437128
14	17.76054797503165	31.65839397313069	32.94775246215178	17.633305589685875
15	17.08379062277122	24.70409748395048	41.33088556513066	16.881226328147644
16	20.37711200260905	21.871384234319528	39.20819413047392	18.5433096325975
17	20.540735940181655	21.363554524176287	32.31472741052209	25.780982125119973
18	22.773828172068924	23.57901426463904	33.525274710959735	20.121882852332305
19	25.995572802417	25.066936008013247	29.652477085902802	19.285014103666953
20	26.91657970607242	24.991544313149326	28.91640198544645	19.17547399533181
21	24.5903935023106	25.005710787130237	31.478789136338236	18.925106574220926
22	22.832820254196324	23.182934539724513	31.22632814764397	22.757917058435194
23	20.488792202251656	26.400398987457667	30.8957073025033	22.215101507787374
24	21.058265846794587	25.07314692517729	34.23120243821533	19.637384789812792
25	21.05684687321029	25.808012408807684	32.24745410550929	20.88768661247273
26	19.339330551524792	28.966926749792155	29.87767517228898	21.81606752639407
27	19.469759811969716	27.49421826419055	32.55323128199378	20.48279064184595
28	18.96062743755237	26.087061705810953	35.622610715995464	19.32970014064122
29	19.24539915261689	25.572928031055515	35.16542207950811	20.01625073681948
30	21.341711635722962	24.746620167755246	33.583359580467665	20.328308616054127
31	25.614380669206554	25.095222432250992	29.126154195439653	20.164242703102804
32	25.492790916335924	25.68907450820934	28.927381584328206	19.890752991126533
33	24.6732522548886	25.767025007897402	28.612555543511082	20.94716719370292
34	21.405076947913436	26.894411151550567	30.514398859982666	21.186113040553334
35	21.267366724650735	26.19813709704058	30.608376782614645	21.926119395694045
36	25.29238997488184	26.193484724633052	28.509482232822393	20.00464306766271
37	21.982227006928778	28.671780244258855	28.915471510964945	20.430521237847422
38	22.16064548875731	29.631052910966154	26.59924138415523	21.60906021612131
39	22.12931176059264	27.81965171409683	28.10453973847154	21.946496786838996
40	22.55160760402356	26.353130883797228	28.813072794275346	22.282188717903864
41	20.473997657995728	25.46566758520006	29.255978647471597	24.804356109332613
42	22.621044262205846	27.273091003660955	27.135915803225117	22.96994893090808
43	22.3209197181965	27.14780261472634	27.799623250882437	22.731654416194722
44	21.468186379621493	28.33041241885681	28.837381440104657	21.364019761417037
45	20.530105269230464	29.576527106349975	27.726208814291713	22.167158810127845
46	22.8976743255572	28.33660007415882	28.53181362037851	20.23391197990547
47	21.57761017864645	27.3271050473123	29.31818086656019	21.77710390748106
48	22.421829675715685	26.043492238214494	29.570083570565558	21.964594515504263
49	21.769287921836423	25.287225841509493	30.324163352239026	22.619322884415062
50	20.33717138549046	27.833050546630496	29.949414754813	21.88036331306605
51	20.61305706925661	28.983233315080525	28.292076870218814	22.111632745444048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3413.0
1	5350.0
2	7287.0
3	46171.5
4	85056.0
5	57542.0
6	30028.0
7	30030.5
8	30033.0
9	30611.5
10	31190.0
11	30845.5
12	30501.0
13	29212.0
14	27923.0
15	26488.0
16	25053.0
17	24133.0
18	23213.0
19	22174.5
20	21136.0
21	21759.0
22	22382.0
23	25003.5
24	27625.0
25	29335.5
26	35440.0
27	39834.0
28	47668.5
29	55503.0
30	64619.5
31	73736.0
32	81661.5
33	89587.0
34	102270.5
35	114954.0
36	125738.0
37	136522.0
38	152833.0
39	169144.0
40	204306.5
41	239469.0
42	268953.0
43	298437.0
44	310895.5
45	323354.0
46	315957.0
47	308560.0
48	316149.5
49	323739.0
50	320539.5
51	317340.0
52	305406.5
53	293473.0
54	267158.5
55	240844.0
56	221235.5
57	201627.0
58	189176.5
59	176726.0
60	162088.0
61	147450.0
62	130312.0
63	113174.0
64	96936.5
65	80699.0
66	67241.0
67	53783.0
68	45239.0
69	36695.0
70	31702.0
71	26709.0
72	23449.0
73	20189.0
74	16236.0
75	8527.5
76	4772.0
77	3685.5
78	2599.0
79	1805.0
80	1011.0
81	678.0
82	345.0
83	253.5
84	162.0
85	191.5
86	221.0
87	122.0
88	23.0
89	19.5
90	16.0
91	10.5
92	5.0
93	4.5
94	4.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4298882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.50186765539997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36959856764689	15.868591424392726
2	6.8108314326351795	2.6564786644497893
3	2.18440445614851	1.2779989982902256
4	1.1720111549392762	0.9142562573711294
5	0.7313992367744824	0.7131825559418251
6	0.5226544304402263	0.6115642519172246
7	0.4169132643138246	0.5691411113100296
8	0.32393041993313915	0.5053798543275373
9	0.2707794369900066	0.4752634269584541
>10	3.3524900756143854	15.891148742322317
>50	1.5867161420448601	22.67511341548486
>100	1.2508095503399486	35.10587306419926
>500	0.004159054001595024	0.5456271715578473
>1k	0.0031804530600432475	0.9713079711940048
>5k	0.0	0.0
>10k+	1.2232511769397108E-4	1.219073090282838
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51102	1.188727673846363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.652372407523631E-5	0.0	0.0	0.02177310286721059	0.0
2	4.652372407523631E-5	0.0	0.0	0.07464731527871665	0.0
3	4.652372407523631E-5	0.0	0.0	0.12140365797432914	0.0
4	4.652372407523631E-5	0.0	0.0	0.18516442181944048	0.0
5	4.652372407523631E-5	0.0	0.0	0.33720395209731274	0.0
6	4.652372407523631E-5	0.0	0.0	0.6049712460123353	0.0
7	4.652372407523631E-5	0.0	0.0	0.8021620505052244	0.0
8	4.652372407523631E-5	0.0	0.0	1.249580704936772	0.0
9	4.652372407523631E-5	0.0	0.0	1.4985989380494742	0.0
10	4.652372407523631E-5	0.0	0.0	1.8547845695694833	0.0
11	4.652372407523631E-5	0.0	0.0	2.0957774602792076	0.0
12	4.652372407523631E-5	0.0	0.0	2.3029010798621594	0.0
13	4.652372407523631E-5	0.0	0.0	2.40436932207025	0.0
14	4.652372407523631E-5	0.0	0.0	2.454196230554828	0.0
15	4.652372407523631E-5	0.0	0.0	2.5032554975921646	0.0
16	9.304744815047262E-5	0.0	0.0	2.5910922886462107	0.0
17	9.304744815047262E-5	0.0	0.0	2.697631616778502	0.0
18	9.304744815047262E-5	0.0	0.0	2.860627484076092	0.0
19	9.304744815047262E-5	0.0	0.0	2.938089484661361	0.0
20	9.304744815047262E-5	0.0	0.0	3.024995801233902	0.0
21	9.304744815047262E-5	0.0	0.0	3.127580612819798	0.0
22	9.304744815047262E-5	0.0	0.0	3.2363065559836257	0.0
23	9.304744815047262E-5	0.0	0.0	3.358780259611685	0.0
24	9.304744815047262E-5	0.0	0.0	3.4545958693446344	0.0
25	9.304744815047262E-5	0.0	0.0	3.5364543618550126	0.0
26	9.304744815047262E-5	0.0	0.0	3.6189874483644817	0.0
27	9.304744815047262E-5	0.0	0.0	3.7080105943824466	0.0
28	9.304744815047262E-5	0.0	0.0	3.80075563832643	0.0
29	9.304744815047262E-5	0.0	0.0	3.905410755633674	0.0
30	9.304744815047262E-5	0.0	0.0	4.046726567512204	0.0
31	9.304744815047262E-5	0.0	0.0	4.164733993629041	0.0
32	9.304744815047262E-5	0.0	0.0	4.276321145823496	0.0
33	9.304744815047262E-5	0.0	0.0	4.396026687869078	0.0
34	9.304744815047262E-5	0.0	0.0	4.520198507425884	0.0
35	9.304744815047262E-5	0.0	0.0	4.658606586549713	0.0
36	9.304744815047262E-5	0.0	0.0	4.778405176043446	0.0
37	9.304744815047262E-5	0.0	0.0	4.909532292349499	0.0
38	1.1630931018809076E-4	0.0	0.0	5.047963633335365	0.0
39	1.1630931018809076E-4	0.0	0.0	5.23854806900957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28320	0.0	43.371296	1
CGGTCTA	520	0.0	40.240383	31
CGTAAGG	700	0.0	39.535713	2
TACGGGA	1500	0.0	39.149998	4
TAGGGAC	3785	0.0	38.10436	5
ACGGGTC	280	0.0	36.964283	5
AGGGCGA	2950	0.0	36.838985	6
ACGGGAT	1500	0.0	36.75	5
TAGCGGG	2445	0.0	36.625767	3
GTTTTTT	35025	0.0	36.60385	2
CGTTAGG	610	0.0	36.516396	2
AGGGATT	5615	0.0	36.2244	6
CGACGGT	610	0.0	36.14754	28
CGTACCG	25	0.0021077492	36.000004	36
TCGCGAT	25	0.0021077492	36.000004	23
TCGTTAG	225	0.0	36.0	1
GTAGGGA	4935	0.0	35.744682	4
GTACGGG	1415	0.0	35.618374	3
GGGCGAT	5995	0.0	35.61718	7
GCGCGAC	2050	0.0	35.560978	9
>>END_MODULE