FastQCFastQC Report
Thu 26 May 2016
SRR1546825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546825_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1843115
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97660.5298638446325921No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38980.21148978766924476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA302.166218E-645.00000441
GTATGCG750.045.0000041
ACGTACA207.0343964E-445.029
GCGTGAT207.0343964E-445.014
CGGTATA207.0343964E-445.045
TATCGCG1050.042.8571431
CGTTTTT60300.042.4253731
CGGTCTA4950.041.8181831
TCACGAC4850.041.7525825
CACGACG4850.041.7525826
CGACGGT4800.041.71875428
TACGGGA4200.041.254
TACGGGC2800.040.982144
ATTAACG501.0822987E-940.51
TAACGCC1950.040.38461712
ACGTTAG850.039.7058831
GCGCGAC9550.039.1099479
AGTCGGC1800.038.7530
GTTGCGA356.250344E-638.57142611
CGACAGG1650.038.1818162
Produced by FastQC (version 0.11.5)