##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546825_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1843115
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.961773410774693	33.0	31.0	34.0	30.0	34.0
2	32.360280828922775	34.0	31.0	34.0	31.0	34.0
3	32.41866079978732	34.0	31.0	34.0	30.0	34.0
4	36.01194173993484	37.0	35.0	37.0	35.0	37.0
5	35.965607137915974	37.0	35.0	37.0	35.0	37.0
6	36.016159056814146	37.0	35.0	37.0	35.0	37.0
7	36.25083567764356	37.0	36.0	37.0	35.0	37.0
8	36.1281195150601	37.0	36.0	37.0	35.0	37.0
9	37.9353621450642	39.0	39.0	39.0	35.0	39.0
10	37.67062934217344	39.0	38.0	39.0	35.0	39.0
11	37.428076924120305	39.0	37.0	39.0	35.0	39.0
12	36.74098252143789	39.0	35.0	39.0	33.0	39.0
13	36.55052940266885	39.0	35.0	39.0	33.0	39.0
14	37.67163579049598	40.0	35.0	41.0	33.0	41.0
15	37.82609658105978	40.0	35.0	41.0	33.0	41.0
16	37.915547863264095	40.0	35.0	41.0	34.0	41.0
17	37.85893500948123	40.0	35.0	41.0	34.0	41.0
18	37.799919701158096	39.0	36.0	41.0	34.0	41.0
19	37.748551229847294	39.0	36.0	41.0	34.0	41.0
20	37.63046635722676	39.0	35.0	41.0	34.0	41.0
21	37.47308116965029	39.0	35.0	41.0	33.0	41.0
22	37.43442161775038	39.0	35.0	41.0	33.0	41.0
23	37.368780027290754	39.0	35.0	41.0	33.0	41.0
24	37.35846705170323	39.0	35.0	41.0	33.0	41.0
25	37.25101797771707	39.0	35.0	41.0	33.0	41.0
26	37.20733595027983	39.0	35.0	41.0	33.0	41.0
27	37.126235747633764	39.0	35.0	41.0	33.0	41.0
28	37.093526990990796	39.0	35.0	41.0	33.0	41.0
29	37.00671146401608	39.0	35.0	41.0	33.0	41.0
30	36.978545017538245	39.0	35.0	41.0	33.0	41.0
31	36.81383418831706	38.0	35.0	41.0	32.0	41.0
32	36.68400940798594	39.0	35.0	41.0	32.0	41.0
33	36.5885324572802	39.0	35.0	41.0	32.0	41.0
34	36.43707256465277	39.0	35.0	41.0	31.0	41.0
35	36.34513201834937	38.0	35.0	41.0	31.0	41.0
36	36.24337927910087	38.0	35.0	41.0	31.0	41.0
37	35.97702313746022	38.0	35.0	41.0	30.0	41.0
38	36.072434980996846	38.0	35.0	41.0	30.0	41.0
39	36.004485341392154	38.0	35.0	41.0	30.0	41.0
40	35.88400778030671	38.0	35.0	41.0	30.0	41.0
41	35.83052658135819	38.0	35.0	41.0	30.0	41.0
42	35.81637608071119	38.0	35.0	40.0	30.0	41.0
43	35.70409117173915	38.0	35.0	40.0	30.0	41.0
44	35.570743008439514	37.0	35.0	40.0	29.0	41.0
45	35.52918727263356	37.0	35.0	40.0	29.0	41.0
46	35.3768522311413	37.0	35.0	40.0	29.0	41.0
47	35.370807573048886	37.0	35.0	40.0	29.0	41.0
48	35.2402639010588	37.0	35.0	40.0	29.0	41.0
49	35.22203714906558	36.0	35.0	40.0	29.0	41.0
50	35.08549439400146	36.0	34.0	40.0	28.0	41.0
51	34.54923485512298	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	7.0
12	6.0
13	6.0
14	16.0
15	52.0
16	108.0
17	233.0
18	592.0
19	1079.0
20	1888.0
21	3102.0
22	4670.0
23	7183.0
24	10214.0
25	15410.0
26	20804.0
27	24854.0
28	26265.0
29	27920.0
30	31744.0
31	38264.0
32	48464.0
33	65602.0
34	131582.0
35	288817.0
36	112113.0
37	147140.0
38	246678.0
39	588049.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.627635823049566	23.61616068449337	28.72994902651218	14.026254465944882
2	32.34800867010469	25.595527137481927	28.39556945714185	13.660894735271537
3	29.16285744514043	25.304064043752017	31.397389745078303	14.13568876602925
4	26.30562932860945	27.039061588669185	30.586642721696695	16.06866636102468
5	23.68132210958079	30.67567677545894	29.919402750235335	15.723598364724936
6	21.590730909357255	39.73588191729762	27.405831974673312	11.267555198671813
7	85.72411379648042	4.708224934418091	7.222066989851419	2.345594279250074
8	86.61109046369869	3.7982437341131727	6.951058398417896	2.6396074037702477
9	81.4814051212214	5.576971594284676	9.166655363338695	3.774967921155218
10	47.789910016466685	25.579141833255115	14.544561788059887	12.08638636221831
11	41.69175553343118	21.110402769224926	22.411298264080102	14.786543433263796
12	36.0217891992632	21.539513269654904	26.128049524853303	16.310648006228586
13	22.881100745205806	34.55101824899695	26.90070885430372	15.66717215149353
14	17.276133068202473	37.76492514031951	29.462024887215392	15.49691690426262
15	15.94241270891941	24.008594146322938	43.822333386685045	16.22665975807261
16	18.41512873586293	20.578965501338768	41.943394742053535	19.062511020744772
17	19.29771066916606	19.95073557537104	29.65935386560253	31.09219988986037
18	22.967476256229265	23.356111799860564	32.43997254647702	21.23643939743315
19	27.93726924255947	25.056873825019053	26.30926447888493	20.696592453536542
20	29.230189109198285	24.87338012006847	25.186382835579984	20.71004793515326
21	24.197296424802577	27.04915319988172	28.966613586238516	19.786936789077185
22	23.622725657378947	23.625546968040517	27.795389869867044	24.95633750471349
23	20.464810931493695	28.51314215336536	27.084799374971176	23.937247540169768
24	20.802391603345423	25.083622020329717	34.668862225091765	19.445124151233102
25	19.845207705433467	25.46048401754638	33.101027336872626	21.593280940147523
26	18.51013094679388	31.05563136320848	28.07258364236632	22.36165404763132
27	19.483374613087083	29.850334895001122	29.96231922587576	20.70397126603603
28	18.183130189923038	26.554935530338586	36.46153387064833	18.800400409090045
29	18.40568819634152	25.363257311670733	35.5507388307295	20.68031566125825
30	19.87922620129509	27.055338380947475	32.36726954096733	20.69816587679011
31	27.465350778437593	25.05497486591992	26.170314928802597	21.309359426839887
32	28.29942787075142	26.262441573097718	26.005648046920566	19.432482509230297
33	26.029466419621127	25.41886968528822	26.796157591902837	21.75550630318781
34	19.97417415625178	27.00797291541765	30.21629144139134	22.80156148693923
35	20.783944572096694	25.25295491599819	30.8936772800395	23.069423231865617
36	27.5138556194269	23.647303613719163	28.365077599607186	20.47376316724675
37	22.24646861427529	28.11685651736327	28.82137034314191	20.81530452521953
38	21.735702872582557	30.580783076476507	25.009888151309063	22.673625899631876
39	21.81448254720948	28.54493615428229	27.133521239857522	22.50706005865071
40	23.06383486651674	25.03728741831085	27.05919055512	24.839687160052414
41	19.98204127251962	24.067407622421825	27.852141618944014	28.09840948611454
42	23.042783548503483	26.694536152112047	25.689064437107834	24.573615862276636
43	22.43115595065962	26.72378012223871	26.345670237614037	24.499393689487633
44	21.723875070193664	28.3938332659655	28.52654337900782	21.355748284833016
45	19.463245646636267	31.922045016181844	25.9694593120885	22.64525002509339
46	22.390301202041112	29.55626751450669	27.37268157439986	20.680749709052336
47	22.171703881743678	26.69187760937326	27.65296793743201	23.483450571451048
48	23.50444763349004	24.22550953141828	29.95776172403784	22.312281111053842
49	22.08706456189657	23.363002308591703	30.819834899070326	23.7300982304414
50	20.56214614931787	29.688597835729187	27.550966705821395	22.198289309131553
51	20.029515249997964	31.165499711086937	25.685483542806608	23.11950149610849
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1440.0
1	2300.5
2	3161.0
3	9903.5
4	16646.0
5	11586.0
6	6526.0
7	6864.0
8	7202.0
9	7625.0
10	8048.0
11	8103.5
12	8159.0
13	7875.5
14	7592.0
15	7338.5
16	7085.0
17	6934.5
18	6784.0
19	6457.0
20	6130.0
21	6735.5
22	7341.0
23	7921.5
24	8502.0
25	10015.5
26	13029.5
27	14530.0
28	16714.0
29	18898.0
30	22605.0
31	26312.0
32	30745.0
33	35178.0
34	39254.0
35	43330.0
36	47487.5
37	51645.0
38	63595.5
39	75546.0
40	95764.5
41	115983.0
42	135904.0
43	155825.0
44	158813.5
45	161802.0
46	158728.0
47	155654.0
48	156969.5
49	158285.0
50	150197.5
51	142110.0
52	130581.5
53	119053.0
54	109130.5
55	99208.0
56	90110.0
57	81012.0
58	75096.5
59	69181.0
60	63750.0
61	58319.0
62	52398.5
63	46478.0
64	41719.5
65	36961.0
66	30069.5
67	23178.0
68	20094.5
69	17011.0
70	14650.5
71	12290.0
72	10687.0
73	9084.0
74	7070.0
75	3696.5
76	2337.0
77	1885.5
78	1434.0
79	986.5
80	539.0
81	519.5
82	500.0
83	322.0
84	144.0
85	92.5
86	41.0
87	29.5
88	18.0
89	16.5
90	15.0
91	13.0
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1843115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.32992373047452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85220694235954	16.437192005787118
2	6.580190240128619	2.6754953142765525
3	2.1122779715466744	1.2882735017731597
4	1.0856534603669115	0.8828500818794022
5	0.7042748607042633	0.715892710170412
6	0.5174246783171527	0.6311522547871816
7	0.4054664692597655	0.5770171676721076
8	0.3438992545942159	0.5593156493493958
9	0.2829286284187117	0.5176725693228157
>10	4.0971870308111145	20.963560595222532
>50	2.2297110500573845	32.731661542314754
>100	0.784458814116362	20.681273798878603
>500	0.0029704120319851285	0.38566132423622645
>1k	0.001080149829812774	0.4168436206299882
>5k	2.700374574531935E-4	0.5361378636997847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9766	0.5298638446325921	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3898	0.21148978766924476	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016873607995160366	0.0
2	0.0	0.0	0.0	0.08507336764119439	0.0
3	0.0	0.0	0.0	0.15001776883157047	0.0
4	0.0	0.0	0.0	0.25522010292358316	0.0
5	0.0	0.0	0.0	0.4846686180732076	0.0
6	0.0	0.0	0.0	0.815630061065099	0.0
7	0.0	0.0	0.0	1.0384593473548855	0.0
8	0.0	0.0	0.0	1.5920330527395197	0.0
9	0.0	0.0	0.0	1.8947271331414481	0.0
10	0.0	0.0	0.0	2.363173214910627	0.0
11	0.0	0.0	0.0	2.6712386367643908	0.0
12	0.0	0.0	0.0	2.9615623550348187	0.0
13	5.425597426096581E-5	0.0	0.0	3.081956361919902	0.0
14	5.425597426096581E-5	0.0	0.0	3.129701619269552	0.0
15	5.425597426096581E-5	0.0	0.0	3.192638549412272	0.0
16	1.0851194852193162E-4	0.0	0.0	3.321008184513717	5.425597426096581E-5
17	1.0851194852193162E-4	0.0	0.0	3.4537182975560397	5.425597426096581E-5
18	1.0851194852193162E-4	0.0	0.0	3.681159341658008	5.425597426096581E-5
19	1.0851194852193162E-4	0.0	0.0	3.7812073581952292	5.425597426096581E-5
20	1.0851194852193162E-4	0.0	0.0	3.8999736858524834	5.425597426096581E-5
21	1.0851194852193162E-4	0.0	0.0	4.033063590714632	5.425597426096581E-5
22	1.0851194852193162E-4	0.0	0.0	4.157309771772244	5.425597426096581E-5
23	1.0851194852193162E-4	0.0	0.0	4.294197594832661	5.425597426096581E-5
24	1.0851194852193162E-4	0.0	0.0	4.4034691269942465	5.425597426096581E-5
25	1.0851194852193162E-4	0.0	0.0	4.493696812190232	5.425597426096581E-5
26	1.0851194852193162E-4	0.0	0.0	4.58511812881996	5.425597426096581E-5
27	1.0851194852193162E-4	0.0	0.0	4.681802274953001	5.425597426096581E-5
28	1.0851194852193162E-4	0.0	0.0	4.777509813549345	5.425597426096581E-5
29	1.0851194852193162E-4	0.0	0.0	4.8822780998472695	5.425597426096581E-5
30	1.0851194852193162E-4	0.0	0.0	5.036093786877108	5.425597426096581E-5
31	1.0851194852193162E-4	0.0	0.0	5.159092080526717	5.425597426096581E-5
32	1.0851194852193162E-4	0.0	0.0	5.277641384286928	5.425597426096581E-5
33	1.0851194852193162E-4	0.0	0.0	5.392881073617219	5.425597426096581E-5
34	1.0851194852193162E-4	0.0	0.0	5.5119729371200386	5.425597426096581E-5
35	1.0851194852193162E-4	0.0	0.0	5.657433204113688	5.425597426096581E-5
36	1.0851194852193162E-4	0.0	0.0	5.77777295502451	5.425597426096581E-5
37	1.0851194852193162E-4	0.0	0.0	5.900065921008727	5.425597426096581E-5
38	1.0851194852193162E-4	0.0	0.0	6.027079156753648	5.425597426096581E-5
39	1.0851194852193162E-4	0.0	0.0	6.166245730733025	5.425597426096581E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	30	2.166218E-6	45.000004	41
GTATGCG	75	0.0	45.000004	1
ACGTACA	20	7.0343964E-4	45.0	29
GCGTGAT	20	7.0343964E-4	45.0	14
CGGTATA	20	7.0343964E-4	45.0	45
TATCGCG	105	0.0	42.857143	1
CGTTTTT	6030	0.0	42.425373	1
CGGTCTA	495	0.0	41.81818	31
TCACGAC	485	0.0	41.75258	25
CACGACG	485	0.0	41.75258	26
CGACGGT	480	0.0	41.718754	28
TACGGGA	420	0.0	41.25	4
TACGGGC	280	0.0	40.98214	4
ATTAACG	50	1.0822987E-9	40.5	1
TAACGCC	195	0.0	40.384617	12
ACGTTAG	85	0.0	39.705883	1
GCGCGAC	955	0.0	39.109947	9
AGTCGGC	180	0.0	38.75	30
GTTGCGA	35	6.250344E-6	38.571426	11
CGACAGG	165	0.0	38.181816	2
>>END_MODULE