FastQCFastQC Report
Thu 26 May 2016
SRR1546824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546824_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1675447
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52610.31400575488212995No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47400.28290957577291315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAT351.2123746E-745.00000412
TACTACG351.2123746E-745.0000041
GCGTATC351.2123746E-745.00000430
CGTATTA253.891573E-545.023
GACGTAT253.891573E-545.026
CGCGATA207.0342125E-445.012
GTCCGAT253.891573E-545.017
GCGATAT1000.045.09
ACGTATC253.891573E-545.027
CGATATC207.0342125E-445.010
TGCGTAT253.891573E-545.018
ACGGTAC207.0342125E-445.034
CGCATCG1150.044.99999621
ATAACGG600.044.9999962
CACGACG1850.042.56756626
TTGAGCG1400.041.7857171
CGTTTTT41850.041.7741931
CGATTCG556.184564E-1140.90909210
ATGTACG501.0822987E-940.51
AACGGGA4350.040.344834
Produced by FastQC (version 0.11.5)