FastQCFastQC Report
Thu 26 May 2016
SRR1546823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546823_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4151877
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT607221.46251924129737No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50490.12160764878150292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT326600.043.8081781
CGGTCTA5150.041.0679631
TCACGAC5350.040.37383325
CGCATTA356.252234E-638.5714311
TATGCGC356.252234E-638.5714329
GTTTTTT392900.038.0363962
TAGGGAC25900.037.5289575
TAACGCC3250.037.38461712
AGGGCGA30950.037.0759286
CGGAATA552.75395E-936.8181824
CGACGGT5950.036.3025228
GGGCGAT58850.036.129997
TCAGACG18250.035.5068522
CACGACG6150.035.48780426
CGCTAAT458.707211E-735.00000416
ATAACGC3600.035.00000411
GTTGATC17950.034.97214516
CTCACGA6200.034.83870724
ACACGCG8150.034.7852836
TATGGGC17950.034.721454
Produced by FastQC (version 0.11.5)