##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546823_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4151877
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954862342983667	33.0	31.0	34.0	30.0	34.0
2	32.356154096087145	34.0	31.0	34.0	31.0	34.0
3	32.37446003337767	34.0	31.0	34.0	30.0	34.0
4	36.007321026128665	37.0	35.0	37.0	35.0	37.0
5	35.970986375559775	37.0	35.0	37.0	35.0	37.0
6	36.014173830294105	37.0	35.0	37.0	35.0	37.0
7	36.239726273201256	37.0	35.0	37.0	35.0	37.0
8	36.10814602648392	37.0	35.0	37.0	35.0	37.0
9	37.90379026160939	39.0	39.0	39.0	35.0	39.0
10	37.667148376505374	39.0	38.0	39.0	35.0	39.0
11	37.48608689515609	39.0	37.0	39.0	35.0	39.0
12	36.988044684368056	39.0	35.0	39.0	33.0	39.0
13	36.85607136242235	39.0	35.0	39.0	33.0	39.0
14	38.03721449358928	40.0	36.0	41.0	33.0	41.0
15	38.14494769474144	40.0	36.0	41.0	33.0	41.0
16	38.20328588732277	40.0	36.0	41.0	34.0	41.0
17	38.14351003172782	40.0	36.0	41.0	34.0	41.0
18	37.971638851536305	39.0	36.0	41.0	34.0	41.0
19	37.8420090479559	39.0	36.0	41.0	34.0	41.0
20	37.66242545239177	39.0	35.0	41.0	34.0	41.0
21	37.52496979077174	39.0	35.0	41.0	33.0	41.0
22	37.509499679301676	39.0	35.0	41.0	33.0	41.0
23	37.42934749752943	39.0	35.0	41.0	33.0	41.0
24	37.38878776996525	39.0	35.0	41.0	33.0	41.0
25	37.272687509769675	39.0	35.0	41.0	33.0	41.0
26	37.2361924498245	39.0	35.0	41.0	33.0	41.0
27	37.171198954111595	39.0	35.0	41.0	33.0	41.0
28	37.087579665775266	39.0	35.0	41.0	33.0	41.0
29	36.95796744460397	39.0	35.0	41.0	33.0	41.0
30	36.85427458472397	39.0	35.0	41.0	32.0	41.0
31	36.623724883950075	39.0	35.0	41.0	31.0	41.0
32	36.368910976890696	39.0	35.0	41.0	31.0	41.0
33	36.10774692988256	39.0	35.0	41.0	30.0	41.0
34	35.822865898965695	39.0	35.0	41.0	29.0	41.0
35	35.610798200428384	38.0	35.0	41.0	27.0	41.0
36	35.492833964011936	38.0	35.0	41.0	26.0	41.0
37	35.23544796726878	38.0	35.0	41.0	24.0	41.0
38	35.29047946266231	38.0	35.0	41.0	25.0	41.0
39	35.17851636741647	38.0	35.0	41.0	25.0	41.0
40	35.04847975987728	38.0	35.0	41.0	24.0	41.0
41	34.953718522971656	38.0	35.0	40.0	23.0	41.0
42	34.93250932048324	38.0	35.0	40.0	24.0	41.0
43	34.81773135379492	37.0	35.0	40.0	23.0	41.0
44	34.65412655529053	37.0	34.0	40.0	23.0	41.0
45	34.5971024671492	37.0	34.0	40.0	23.0	41.0
46	34.41330342878655	36.0	34.0	40.0	23.0	41.0
47	34.38034026537877	36.0	34.0	40.0	23.0	41.0
48	34.221326402492174	36.0	34.0	40.0	23.0	41.0
49	34.17307015598006	36.0	34.0	40.0	23.0	41.0
50	34.03730722273323	35.0	34.0	40.0	23.0	41.0
51	33.49821369949061	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	14.0
12	23.0
13	25.0
14	63.0
15	131.0
16	332.0
17	717.0
18	1559.0
19	3059.0
20	5313.0
21	8536.0
22	13185.0
23	19831.0
24	29817.0
25	48581.0
26	71813.0
27	84916.0
28	84029.0
29	80954.0
30	81415.0
31	92295.0
32	111261.0
33	145396.0
34	268603.0
35	505839.0
36	278548.0
37	363636.0
38	593767.0
39	1257673.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.88533764367297	21.572098595406366	27.723870432577847	18.81869332834282
2	36.41810679844321	23.694873427127057	27.116867864823547	12.770151909606186
3	28.693817278305687	23.77657141577171	34.015241781006516	13.51436952491608
4	25.76791171800128	25.544615122268794	33.46382852863897	15.223644631090949
5	22.90065433055941	29.466696629018635	32.80884766094949	14.823801379472465
6	20.573297330339987	37.644877244677524	30.937091826178857	10.844733598803625
7	80.88079198878	4.878709075437446	11.991949665175534	2.248549270607005
8	81.47110331062312	3.859290629274422	11.973741033272422	2.6958650268300337
9	76.25324642324424	5.633331623263406	13.979195433776098	4.134226519716263
10	42.29751989280992	23.562523648942392	20.43911705476824	13.70083940347944
11	35.877676530398176	21.777355157679285	26.58982913029456	15.755139181627973
12	31.3203401738539	20.94924777395862	30.89005286042915	16.84035919175833
13	23.9160986705531	26.920161652187673	31.473042192723916	17.69069748453531
14	19.170895476913213	30.064618966313304	33.43846650563106	17.32601905114241
15	17.992585040452788	22.261160434184347	42.26999017552784	17.476264349835027
16	21.27565436066627	19.934477827739116	39.759390752664395	19.030477058930213
17	21.52127820742281	20.15844881724579	32.2372266808482	26.083046294483193
18	23.622400181893635	22.053471237225956	34.33480808800453	19.989320492875873
19	26.915031442405446	23.929514289561084	29.499139786655533	19.65631448137794
20	27.48393076191804	23.46683198948331	28.891510996110913	20.15772625248773
21	25.199879476198355	24.435213278235363	31.350880577627905	19.01402666793838
22	23.46781949465266	22.14343054960443	31.31289775684588	23.075852198897028
23	21.842915866727267	25.277723786133354	30.280063691674876	22.599296655464503
24	22.084565607314474	23.66996902846592	34.36166341151243	19.883801952707174
25	21.95946556220235	23.66780133419174	33.14664668534256	21.226086418263353
26	19.817904046772096	27.166243123290982	30.87719120773568	22.138661622201237
27	20.384683842994384	25.374523378221465	32.86400343748141	21.37678934130274
28	19.881272976053964	24.849050200668277	35.83586411639843	19.433812706879323
29	20.622624417823555	24.547523927129824	34.87771916171891	19.952132493327717
30	22.294181643627688	24.270227658478323	33.0869628363268	20.348627861567188
31	26.649199867915165	23.55975381736983	29.2809252297214	20.510121084993607
32	27.16238944458133	24.769977530644574	28.625149540798056	19.44248348397604
33	26.105036348620153	24.62308011533097	28.61520223262876	20.656681303420115
34	22.801518445753572	26.051759240459194	29.927717030152866	21.219005283634367
35	22.981798352889548	25.79806675390432	30.193235493247993	21.02689939995814
36	26.557506400117344	25.49401150371266	27.628660482957468	20.31982161321253
37	23.88105427978719	28.17983288040566	27.865830322044705	20.073282517762447
38	23.66618760623207	28.78153663993418	25.882486403137666	21.669789350696085
39	23.058631072163266	26.882732797720166	27.361383778951065	22.69725235116551
40	23.944423209069054	24.581339957807035	28.615298574596505	22.85893825852741
41	21.058836762264395	24.89052541778092	28.845700390449906	25.204937429504774
42	23.087702260929213	26.605532871036402	27.45762940472466	22.84913546330973
43	22.682463859117213	25.60894747122807	28.11152642527705	23.597062244377664
44	22.187362486894482	27.272821425104837	28.73466627262802	21.805149815372662
45	21.80685988530007	28.56341842496779	27.3124661448304	22.31725554490174
46	23.54761472943442	26.894125235405575	28.558336386169437	20.999923648990563
47	22.841572618842033	26.274935408732002	28.809331297627555	22.07416067479841
48	23.210152901928453	24.98571128190936	29.778122039742506	22.026013776419678
49	22.94343016423656	24.211627656599653	30.183529039998053	22.66141313916573
50	21.456536405100632	26.335462249965495	29.47958236720404	22.72841897772983
51	21.400730320286463	28.13850217624462	28.076000324672428	22.384767178796483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3813.0
1	5920.0
2	8027.0
3	54263.5
4	100500.0
5	67462.0
6	34424.0
7	34380.0
8	34336.0
9	35005.5
10	35675.0
11	35139.5
12	34604.0
13	32717.0
14	30830.0
15	28921.5
16	27013.0
17	25682.0
18	24351.0
19	22695.5
20	21040.0
21	20742.0
22	20444.0
23	21853.0
24	23262.0
25	24290.0
26	30146.0
27	34974.0
28	38342.0
29	41710.0
30	48642.5
31	55575.0
32	63038.5
33	70502.0
34	77572.0
35	84642.0
36	93965.5
37	103289.0
38	119358.0
39	135427.0
40	162599.0
41	189771.0
42	225028.0
43	260285.0
44	265375.5
45	270466.0
46	277111.0
47	283756.0
48	290511.0
49	297266.0
50	295683.0
51	294100.0
52	288856.5
53	283613.0
54	261656.0
55	239699.0
56	226325.0
57	212951.0
58	207770.0
59	202589.0
60	187316.5
61	172044.0
62	156466.5
63	140889.0
64	127504.5
65	114120.0
66	98012.5
67	81905.0
68	67422.5
69	52940.0
70	47514.5
71	42089.0
72	35354.0
73	28619.0
74	23136.0
75	12891.0
76	8129.0
77	6306.0
78	4483.0
79	3538.5
80	2594.0
81	1946.0
82	1298.0
83	888.0
84	478.0
85	335.0
86	192.0
87	137.5
88	83.0
89	71.5
90	60.0
91	42.5
92	25.0
93	18.5
94	12.0
95	10.0
96	8.0
97	6.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4151877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.96249732639162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59070061745192	17.10344843551755
2	6.609533752662205	2.7710466723775333
3	2.109467976122005	1.326591504286988
4	1.1428167604245298	0.9582517313981896
5	0.7265508536155116	0.7615160163201357
6	0.5256959783426195	0.6611940324301193
7	0.42345044533364995	0.6213605179716185
8	0.3466545894516197	0.581339672364877
9	0.2909498214267482	0.5489131368395096
>10	3.5839548257391884	18.47057602923772
>50	1.700882306084929	25.980100024880535
>100	0.9412398044621015	26.815955700580467
>500	0.004227270721581357	0.6030685289937563
>1k	0.00364014978802835	1.177682069435797
>5k	1.1742418671059197E-4	0.1242813470567122
>10k+	1.1742418671059197E-4	1.4946745803085124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60722	1.46251924129737	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5049	0.12160764878150292	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.225647580600292E-5	0.0	0.0	0.018545828790207417	0.0
2	7.225647580600292E-5	0.0	0.0	0.07721808714468179	0.0
3	7.225647580600292E-5	0.0	0.0	0.14985993082165006	0.0
4	7.225647580600292E-5	0.0	0.0	0.22341702319216103	0.0
5	7.225647580600292E-5	0.0	0.0	0.395242922658836	0.0
6	7.225647580600292E-5	0.0	0.0	0.7316449885196503	0.0
7	7.225647580600292E-5	0.0	0.0	0.9847112522842079	0.0
8	7.225647580600292E-5	0.0	0.0	1.4881702902085008	0.0
9	7.225647580600292E-5	0.0	0.0	1.8252949208273752	0.0
10	7.225647580600292E-5	0.0	0.0	2.301368754421193	0.0
11	7.225647580600292E-5	0.0	0.0	2.6328814654191346	0.0
12	7.225647580600292E-5	0.0	0.0	2.8958468663691144	0.0
13	7.225647580600292E-5	0.0	0.0	3.019429525489315	0.0
14	7.225647580600292E-5	0.0	0.0	3.0778368434325007	0.0
15	7.225647580600292E-5	0.0	0.0	3.135714280553109	0.0
16	1.9268393548267445E-4	0.0	0.0	3.2374995694718316	0.0
17	1.9268393548267445E-4	0.0	0.0	3.343451648495367	0.0
18	1.9268393548267445E-4	0.0	0.0	3.5160723691959084	0.0
19	1.9268393548267445E-4	0.0	0.0	3.596686510703472	0.0
20	1.9268393548267445E-4	0.0	0.0	3.6907885276948234	0.0
21	2.1676942741800877E-4	0.0	0.0	3.7936817492425714	0.0
22	2.1676942741800877E-4	0.0	0.0	3.909557050943465	0.0
23	2.1676942741800877E-4	0.0	0.0	4.034488497612044	0.0
24	2.1676942741800877E-4	0.0	0.0	4.133431698482397	0.0
25	2.1676942741800877E-4	0.0	0.0	4.221126974618949	0.0
26	2.1676942741800877E-4	0.0	0.0	4.308870421739372	0.0
27	2.1676942741800877E-4	0.0	0.0	4.4037190889807185	0.0
28	2.4085491935334308E-4	0.0	0.0	4.4970021992462685	0.0
29	2.4085491935334308E-4	0.0	0.0	4.596619793890811	0.0
30	2.4085491935334308E-4	0.0	0.0	4.743709893139898	0.0
31	2.4085491935334308E-4	0.0	0.0	4.85339522341341	0.0
32	2.4085491935334308E-4	0.0	0.0	4.965248247961103	0.0
33	2.4085491935334308E-4	0.0	0.0	5.079341223258782	0.0
34	2.4085491935334308E-4	0.0	0.0	5.196155859145153	0.0
35	2.4085491935334308E-4	0.0	0.0	5.327831243555625	0.0
36	2.4085491935334308E-4	0.0	0.0	5.444453195506514	0.0
37	2.4085491935334308E-4	0.0	0.0	5.566759323554142	0.0
38	2.4085491935334308E-4	0.0	0.0	5.707201827029076	0.0
39	2.4085491935334308E-4	0.0	0.0	5.899548565624656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32660	0.0	43.808178	1
CGGTCTA	515	0.0	41.06796	31
TCACGAC	535	0.0	40.373833	25
CGCATTA	35	6.252234E-6	38.57143	11
TATGCGC	35	6.252234E-6	38.57143	29
GTTTTTT	39290	0.0	38.036396	2
TAGGGAC	2590	0.0	37.528957	5
TAACGCC	325	0.0	37.384617	12
AGGGCGA	3095	0.0	37.075928	6
CGGAATA	55	2.75395E-9	36.81818	24
CGACGGT	595	0.0	36.30252	28
GGGCGAT	5885	0.0	36.12999	7
TCAGACG	1825	0.0	35.50685	22
CACGACG	615	0.0	35.487804	26
CGCTAAT	45	8.707211E-7	35.000004	16
ATAACGC	360	0.0	35.000004	11
GTTGATC	1795	0.0	34.972145	16
CTCACGA	620	0.0	34.838707	24
ACACGCG	815	0.0	34.78528	36
TATGGGC	1795	0.0	34.72145	4
>>END_MODULE