FastQCFastQC Report
Thu 26 May 2016
SRR1546822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546822_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences585369
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88971.5198959972256816No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG6390.10916191325471625No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6260.10694109185829792No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC6140.10489110287698872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG700.045.0000042
GAGTACG253.8884507E-545.0000041
GTAAGCG253.8884507E-545.0000041
TACGTTG351.2105556E-745.0000041
CCGTCGA207.030447E-445.041
CGTCGAA207.030447E-445.042
AACCCGA302.1636697E-644.99999624
CGTTTTT45600.044.309211
TAACGGG1150.041.0869563
CGACGGT501.0804797E-940.50000428
GCGTAAG451.9255822E-840.01
CGATGAA3200.039.37519
TGGGCGA1900.039.078956
TACGGGA1500.039.04
GGGCGAT7450.038.9597327
AACGGGC700.038.571434
ACTACGG356.2430354E-638.571432
TAGTACG356.2430354E-638.571431
AGGGTCC1650.038.1818166
ACCCGCT2600.038.07692734
Produced by FastQC (version 0.11.5)