##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546822_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	585369
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92675218537367	33.0	31.0	34.0	30.0	34.0
2	32.32573983248173	34.0	31.0	34.0	31.0	34.0
3	32.34327919654098	34.0	31.0	34.0	30.0	34.0
4	35.98050460478775	37.0	35.0	37.0	35.0	37.0
5	35.94111064986359	37.0	35.0	37.0	35.0	37.0
6	35.99545414943395	37.0	35.0	37.0	35.0	37.0
7	36.222405012906385	37.0	35.0	37.0	35.0	37.0
8	36.08920868716997	37.0	36.0	37.0	35.0	37.0
9	37.91914501792886	39.0	39.0	39.0	35.0	39.0
10	37.64552786362107	39.0	37.0	39.0	35.0	39.0
11	37.434941037191926	39.0	37.0	39.0	35.0	39.0
12	36.99586927220266	39.0	35.0	39.0	33.0	39.0
13	36.890088132442955	39.0	35.0	39.0	33.0	39.0
14	38.09013459885986	40.0	36.0	41.0	33.0	41.0
15	38.20396536201951	40.0	36.0	41.0	33.0	41.0
16	38.271609873430265	40.0	36.0	41.0	34.0	41.0
17	38.18520112954393	40.0	36.0	41.0	34.0	41.0
18	38.042976310669	40.0	36.0	41.0	34.0	41.0
19	37.923077580124676	40.0	36.0	41.0	34.0	41.0
20	37.69576967690465	40.0	35.0	41.0	34.0	41.0
21	37.58915145831091	39.0	35.0	41.0	33.0	41.0
22	37.56363763711437	39.0	35.0	41.0	33.0	41.0
23	37.45334139662333	39.0	35.0	41.0	33.0	41.0
24	37.447919175767765	39.0	35.0	41.0	33.0	41.0
25	37.37003326107122	39.0	35.0	41.0	33.0	41.0
26	37.31139161793672	39.0	35.0	41.0	33.0	41.0
27	37.220939270784754	39.0	35.0	41.0	33.0	41.0
28	37.1695716718856	39.0	35.0	41.0	33.0	41.0
29	37.04545338068808	39.0	35.0	41.0	33.0	41.0
30	36.98168164012785	39.0	35.0	41.0	33.0	41.0
31	36.764384516433225	39.0	35.0	41.0	32.0	41.0
32	36.485485223850254	39.0	35.0	41.0	31.0	41.0
33	36.21757728885541	39.0	35.0	41.0	30.0	41.0
34	35.94449654833105	39.0	35.0	41.0	29.0	41.0
35	35.73573762874358	39.0	35.0	41.0	27.0	41.0
36	35.61312266279902	39.0	35.0	41.0	26.0	41.0
37	35.349757161721925	39.0	35.0	41.0	24.0	41.0
38	35.432422967393215	39.0	35.0	41.0	25.0	41.0
39	35.33301387671708	38.0	35.0	41.0	24.0	41.0
40	35.21967169426464	38.0	35.0	41.0	24.0	41.0
41	35.12337517019179	38.0	35.0	41.0	23.0	41.0
42	35.11710732888144	38.0	35.0	41.0	23.0	41.0
43	34.98795460641066	38.0	35.0	41.0	23.0	41.0
44	34.8702715722903	38.0	35.0	40.0	23.0	41.0
45	34.83703612593082	38.0	34.0	40.0	23.0	41.0
46	34.6663232935123	38.0	34.0	40.0	23.0	41.0
47	34.655282394523795	37.0	34.0	40.0	23.0	41.0
48	34.52915682244875	37.0	34.0	40.0	23.0	41.0
49	34.48730629739532	37.0	34.0	40.0	23.0	41.0
50	34.35701412271576	37.0	34.0	40.0	23.0	41.0
51	33.81786360398313	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	0.0
14	9.0
15	12.0
16	30.0
17	89.0
18	209.0
19	476.0
20	813.0
21	1321.0
22	2144.0
23	3034.0
24	4461.0
25	7092.0
26	10293.0
27	12002.0
28	11501.0
29	10654.0
30	11133.0
31	12269.0
32	14797.0
33	19555.0
34	35556.0
35	68370.0
36	36658.0
37	46665.0
38	80420.0
39	195726.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07737341745121	21.93317377585762	27.528618700341152	18.46083410635001
2	35.25724799229204	24.5265806696289	28.207848382814944	12.008322955264115
3	27.601734974007847	24.696558922662458	34.83751274836898	12.864193354960719
4	24.51205991434463	26.085938954744787	35.35940577652729	14.042595354383302
5	22.992163917118948	30.115021465092955	32.92743551503411	13.965379102753989
6	19.72243149193073	38.79382064988067	30.95056280739158	10.533185050797018
7	82.32721582454828	3.743109047455537	11.991410546168314	1.9382645818278728
8	83.21315272930408	3.7947004368184856	11.027574060122761	1.9645727737546743
9	78.77748907099624	5.172463864673394	12.558744996745643	3.49130206758472
10	40.518032215576845	29.662486397468946	18.925327443031662	10.894153943922552
11	33.11654699855988	22.952872461643853	29.61072417569089	14.319856364105377
12	29.96503060462717	21.623967104510147	31.375081358937695	17.03592093192499
13	23.049392776180493	28.86281986234324	32.025782028088265	16.062005333388
14	17.17685767439	32.383505105326726	33.747260275142686	16.692376945140587
15	16.54119025776903	24.350452449651417	42.40504707287198	16.703310219707568
16	20.184533174800855	21.617475472736	39.63807444534986	18.55991690711329
17	19.624544518073215	22.062323081680102	33.0080684149656	25.30506398528108
18	21.862107491172235	23.68181437691439	34.292557344170945	20.163520787742435
19	25.2717516643348	25.227506068821548	30.072142528900574	19.428599737943074
20	26.068001551158332	25.4668422823894	30.172250324154508	18.292905842297763
21	23.403015875456337	25.511087877902654	32.64163288455658	18.444263362084428
22	21.73176235844399	23.281895693143984	32.484808727486424	22.501533220925605
23	19.976459293197966	26.949325980706185	31.87220368690518	21.202011039190662
24	20.34255315877677	25.47606723280529	35.648625055306994	18.532754553110944
25	19.89360557187005	26.17220932437488	33.91023439915677	20.023950704598295
26	18.665491339650718	29.308863298193106	31.29034848104358	20.735296881112596
27	19.30440457215876	27.42082344640731	33.394833002772614	19.879938978661325
28	17.879833062563954	26.801385109221705	36.81660627740793	18.502175550806417
29	18.50849635016545	25.140723201946123	36.83573950790014	19.515040939988282
30	20.266874398883438	25.90058578435141	34.65540539386268	19.17713442290248
31	24.50010164528699	25.486317177711836	29.830414661521193	20.183166515479982
32	25.757769885320197	26.27402544377991	29.792148200536754	18.17605647036314
33	23.70419342329368	26.07517651259291	29.96451810738184	20.256111956731566
34	20.12030018671983	27.51512293954753	31.7205045022883	20.644072371444334
35	20.518852211169364	26.617056933318985	31.776366702028973	21.087724153482675
36	24.70783386205966	26.53249488783998	29.044585552019324	19.71508569808104
37	21.041599401403218	29.17561401440801	29.791294038461206	19.991492545727567
38	20.72897608175356	29.861847825901265	27.380848661271777	22.028327431073393
39	20.76963419654953	28.34981011977061	28.832411692453817	22.048143991226045
40	22.039089873225265	26.48824929232672	28.256877285951255	23.21578354849676
41	19.800160240805372	26.27180462238349	29.21507630229821	24.712958834512932
42	22.122456091798508	27.826721264706535	27.446789973503893	22.604032669991064
43	21.72800404531159	27.87182102229534	28.35288510324257	22.0472898291505
44	21.47893038408252	28.81584094818824	29.438012604015583	20.267216063713658
45	20.075200429131026	30.038317710708974	27.963216364378706	21.923265495781294
46	22.364696456423214	28.800295198413306	28.606912904509805	20.228095440653675
47	20.909375112108773	27.705601082394182	29.98963047240288	21.395393333094166
48	22.49504158915146	26.14333864622144	30.119804772716012	21.241814991911085
49	21.172286198961682	25.39765515427021	31.13420765363386	22.295850993134245
50	20.071271283583517	28.695916592781646	29.795735681254047	21.43707644238079
51	19.575515614936904	30.014572005008805	28.408405638153027	22.00150674190126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	603.0
1	809.0
2	1015.0
3	7644.5
4	14274.0
5	9592.0
6	4910.0
7	4837.0
8	4764.0
9	5006.5
10	5249.0
11	5116.0
12	4983.0
13	4723.5
14	4464.0
15	4261.5
16	4059.0
17	3797.5
18	3536.0
19	3361.0
20	3186.0
21	3241.5
22	3297.0
23	3463.0
24	3629.0
25	4400.0
26	5393.0
27	5615.0
28	6888.0
29	8161.0
30	9386.5
31	10612.0
32	12348.5
33	14085.0
34	15401.0
35	16717.0
36	18061.5
37	19406.0
38	21710.5
39	24015.0
40	28471.0
41	32927.0
42	36731.0
43	40535.0
44	41808.0
45	43081.0
46	45368.5
47	47656.0
48	46216.5
49	44777.0
50	43555.5
51	42334.0
52	39676.0
53	37018.0
54	32805.0
55	28592.0
56	27014.5
57	25437.0
58	23527.0
59	21617.0
60	19524.0
61	17431.0
62	14875.0
63	12319.0
64	10932.0
65	9545.0
66	8118.5
67	6692.0
68	5639.5
69	4587.0
70	4027.5
71	3468.0
72	2983.0
73	2498.0
74	2028.0
75	1120.5
76	683.0
77	574.5
78	466.0
79	340.5
80	215.0
81	173.0
82	131.0
83	79.0
84	27.0
85	22.0
86	17.0
87	11.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	585369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.22264083578425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07296655256869	23.546039560286957
2	6.347255539391211	4.0905067107749
3	3.227397042576706	3.1198576721226443
4	2.2354327516704013	2.8812618667849685
5	1.7022148352409954	2.7424928630657117
6	1.382588941001207	2.673040012164552
7	1.2390312922073605	2.794740221916679
8	1.1431301879760192	2.9467738780555033
9	0.949913627485687	2.754785307913942
>10	8.645995350875772	48.72848718934467
>50	0.03158771149851639	0.6886805447295662
>100	0.020344627744800763	1.1743107384701956
>500	0.0016061548219579549	0.3241559978824001
>1k	0.0	0.0
>5k	5.353849406526517E-4	1.5348674364873411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8897	1.5198959972256816	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	639	0.10916191325471625	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	626	0.10694109185829792	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	614	0.10489110287698872	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026991521587238134	0.0
2	0.0	0.0	0.0	0.09822863868773372	0.0
3	0.0	0.0	0.0	0.16468244816517444	0.0
4	0.0	0.0	0.0	0.2567611199089805	0.0
5	0.0	0.0	0.0	0.5083972673646879	0.0
6	0.0	0.0	0.0	0.8187997656179264	0.0
7	0.0	0.0	0.0	1.0595026385066513	0.0
8	0.0	0.0	0.0	1.5299751097171186	0.0
9	0.0	0.0	0.0	1.8181693940061738	0.0
10	0.0	0.0	0.0	2.311875073671479	0.0
11	0.0	0.0	0.0	2.5752986577697143	0.0
12	0.0	0.0	0.0	2.8564888130392965	0.0
13	0.0	0.0	0.0	2.9567674407083397	0.0
14	0.0	0.0	0.0	3.010921316297925	0.0
15	0.0	0.0	0.0	3.0671251808688194	0.0
16	1.7083241510910211E-4	0.0	0.0	3.1460497566492247	0.0
17	1.7083241510910211E-4	0.0	0.0	3.2480367084693587	0.0
18	1.7083241510910211E-4	0.0	0.0	3.416135804936715	0.0
19	1.7083241510910211E-4	0.0	0.0	3.508043644265412	0.0
20	1.7083241510910211E-4	0.0	0.0	3.593459851819963	0.0
21	1.7083241510910211E-4	0.0	0.0	3.6810968807709323	0.0
22	1.7083241510910211E-4	0.0	0.0	3.7981170851206674	0.0
23	1.7083241510910211E-4	0.0	0.0	3.900958199016347	0.0
24	1.7083241510910211E-4	0.0	0.0	3.987741065891771	0.0
25	1.7083241510910211E-4	0.0	0.0	4.068886463068594	0.0
26	1.7083241510910211E-4	0.0	0.0	4.148665200924545	0.0
27	1.7083241510910211E-4	0.0	0.0	4.236814727120842	0.0
28	1.7083241510910211E-4	0.0	0.0	4.325647582977575	0.0
29	1.7083241510910211E-4	0.0	0.0	4.415676265740071	0.0
30	1.7083241510910211E-4	0.0	0.0	4.550804706091371	0.0
31	1.7083241510910211E-4	0.0	0.0	4.653304155156833	0.0
32	1.7083241510910211E-4	0.0	0.0	4.750166134523694	0.0
33	1.7083241510910211E-4	0.0	0.0	4.852836416004264	0.0
34	1.7083241510910211E-4	0.0	0.0	4.963364988579853	0.0
35	1.7083241510910211E-4	0.0	0.0	5.086022662628189	0.0
36	1.7083241510910211E-4	0.0	0.0	5.187667949618104	0.0
37	1.7083241510910211E-4	0.0	0.0	5.312888109893076	0.0
38	1.7083241510910211E-4	0.0	0.0	5.446137393678176	0.0
39	1.7083241510910211E-4	0.0	0.0	5.638153028260806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	70	0.0	45.000004	2
GAGTACG	25	3.8884507E-5	45.000004	1
GTAAGCG	25	3.8884507E-5	45.000004	1
TACGTTG	35	1.2105556E-7	45.000004	1
CCGTCGA	20	7.030447E-4	45.0	41
CGTCGAA	20	7.030447E-4	45.0	42
AACCCGA	30	2.1636697E-6	44.999996	24
CGTTTTT	4560	0.0	44.30921	1
TAACGGG	115	0.0	41.086956	3
CGACGGT	50	1.0804797E-9	40.500004	28
GCGTAAG	45	1.9255822E-8	40.0	1
CGATGAA	320	0.0	39.375	19
TGGGCGA	190	0.0	39.07895	6
TACGGGA	150	0.0	39.0	4
GGGCGAT	745	0.0	38.959732	7
AACGGGC	70	0.0	38.57143	4
ACTACGG	35	6.2430354E-6	38.57143	2
TAGTACG	35	6.2430354E-6	38.57143	1
AGGGTCC	165	0.0	38.181816	6
ACCCGCT	260	0.0	38.076927	34
>>END_MODULE