FastQCFastQC Report
Thu 26 May 2016
SRR1546821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546821_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2103777
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101460.4822754502972511No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50800.24147046003449987No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG41530.1974068544337161No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC28760.13670650453921684No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT25110.11935675691862778No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC21950.10433615349915891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA351.2125747E-745.00000432
TCGATAG850.045.0000041
TACGACC351.2125747E-745.00000431
GATATAC351.2125747E-745.00000411
ACACGAT207.034624E-445.044
ACGCCGT253.891915E-545.040
GACCGTT253.891915E-545.044
TAAGTCG406.8193913E-945.01
TCGCTAC207.034624E-445.019
ACGTACT207.034624E-445.030
CAATACG253.891915E-545.01
TCGGTAA207.034624E-445.012
TACACGA207.034624E-445.034
TAATCGC207.034624E-445.028
TATGCGA302.1663654E-644.99999619
TCACGAC4450.043.48314725
CGACGGT4550.042.5274728
ATTAGCG1800.042.5000041
TAACGCC1700.042.35294312
CGTTTTT67450.041.9977761