##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546821_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2103777
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00448716760379	33.0	31.0	34.0	30.0	34.0
2	32.3908826838586	34.0	31.0	34.0	31.0	34.0
3	32.44261582857879	34.0	31.0	34.0	31.0	34.0
4	36.027258592521925	37.0	35.0	37.0	35.0	37.0
5	35.97994036440174	37.0	35.0	37.0	35.0	37.0
6	36.042020138065965	37.0	35.0	37.0	35.0	37.0
7	36.26034365809684	37.0	36.0	37.0	35.0	37.0
8	36.13946630274977	37.0	36.0	37.0	35.0	37.0
9	38.00337155506501	39.0	39.0	39.0	35.0	39.0
10	37.71969890344842	39.0	38.0	39.0	35.0	39.0
11	37.456611608549764	39.0	37.0	39.0	35.0	39.0
12	36.53829279434085	38.0	35.0	39.0	33.0	39.0
13	36.22841394311279	38.0	35.0	39.0	33.0	39.0
14	37.28424448028475	39.0	35.0	41.0	33.0	41.0
15	37.49528300765718	39.0	35.0	41.0	33.0	41.0
16	37.64521001988329	39.0	35.0	41.0	34.0	41.0
17	37.56603004976288	39.0	35.0	41.0	34.0	41.0
18	37.544710774953806	39.0	35.0	41.0	34.0	41.0
19	37.50993950404439	39.0	35.0	41.0	34.0	41.0
20	37.32937473886253	39.0	35.0	41.0	34.0	41.0
21	37.198112252391766	39.0	35.0	41.0	33.0	41.0
22	37.14239389440991	38.0	35.0	41.0	33.0	41.0
23	37.08548719754993	38.0	35.0	41.0	33.0	41.0
24	37.073409871863795	38.0	35.0	41.0	33.0	41.0
25	36.98706516897941	38.0	35.0	41.0	33.0	41.0
26	36.9069140883278	38.0	35.0	41.0	33.0	41.0
27	36.84283933135499	38.0	35.0	41.0	33.0	41.0
28	36.836452247552856	38.0	35.0	41.0	33.0	41.0
29	36.77192924915521	38.0	35.0	41.0	33.0	41.0
30	36.76934152241421	38.0	35.0	41.0	33.0	41.0
31	36.57404705917025	38.0	35.0	41.0	32.0	41.0
32	36.388824956257245	38.0	35.0	41.0	32.0	41.0
33	36.28201563188494	38.0	35.0	41.0	31.0	41.0
34	36.15616769267845	38.0	35.0	41.0	31.0	41.0
35	36.04254395784344	38.0	35.0	41.0	31.0	41.0
36	35.93302759750677	38.0	35.0	41.0	31.0	41.0
37	35.667404387442204	37.0	35.0	40.0	30.0	41.0
38	35.76026166271425	37.0	35.0	40.0	30.0	41.0
39	35.71585676618767	37.0	35.0	40.0	30.0	41.0
40	35.57784451488917	37.0	35.0	40.0	30.0	41.0
41	35.54655935491262	37.0	35.0	40.0	30.0	41.0
42	35.528633975939464	36.0	35.0	40.0	30.0	41.0
43	35.41307990343083	36.0	35.0	40.0	30.0	41.0
44	35.29210415362465	36.0	35.0	40.0	30.0	41.0
45	35.24747965207339	36.0	35.0	40.0	29.0	41.0
46	35.10043745130781	35.0	35.0	40.0	29.0	41.0
47	35.07759615206365	35.0	35.0	40.0	29.0	41.0
48	34.994316888149264	35.0	35.0	40.0	29.0	41.0
49	34.9945008430076	36.0	35.0	40.0	29.0	41.0
50	34.84457192943929	35.0	34.0	40.0	29.0	41.0
51	34.31053671563098	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	4.0
12	4.0
13	16.0
14	14.0
15	36.0
16	99.0
17	244.0
18	524.0
19	1178.0
20	2174.0
21	3761.0
22	6337.0
23	8963.0
24	12416.0
25	17940.0
26	24393.0
27	28680.0
28	29632.0
29	31331.0
30	35992.0
31	44027.0
32	56765.0
33	79517.0
34	168455.0
35	413785.0
36	120706.0
37	154172.0
38	253323.0
39	608967.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3592391208764	22.90047852029944	30.267038759336184	13.473243599487969
2	30.497624035247085	25.747928606501546	30.73724068663171	13.017206671619663
3	28.49593849538235	25.44019637062293	32.64224297537239	13.421622158622327
4	25.39190227861603	26.460646732044317	32.452821758199654	15.694629231139992
5	23.378428417080325	31.226788770863074	29.83709775323145	15.557685058825152
6	20.184838982458693	40.542177236465655	28.037619956868053	11.235363824207603
7	86.14891217082419	4.462877957121882	7.746400878039831	1.6418089940140994
8	87.51464627667286	4.149346627518031	6.121799030980945	2.214208064828164
9	83.710916128468	4.601438270310969	7.718451147626388	3.9691944535946537
10	54.51628190630471	20.813089980544515	13.487741333801065	11.182886779349714
11	47.45645569848896	19.318872675193237	18.958425726681106	14.2662458996367
12	41.04993067230985	20.409149829093103	23.559959064102326	14.980960434494719
13	22.40432327190572	39.29090393135775	23.50258606306657	14.802186733669966
14	14.840926581096761	42.14767059436433	27.463462144514367	15.547940680024547
15	13.27802328859	22.617178531755027	48.32147133465192	15.78332684500306
16	15.333659413521492	17.03588355609934	45.93737834380735	21.693078686571816
17	16.0061641514286	18.634674682725404	28.61291857454474	36.74624259130127
18	21.104993542566536	22.45670524965336	33.6377382203532	22.8005629874269
19	28.25879358886422	24.96324467849967	24.796782168452264	21.981179564183847
20	31.125257097116283	22.887882128191343	24.08558511667349	21.901275658018886
21	22.275697471737736	28.425446233132124	26.994163354766215	22.30469294036393
22	22.108854693249334	23.296385500934747	26.149587147306963	28.44517265850896
23	19.361510274140272	30.50161685387757	24.682654102597375	25.454218769384777
24	20.345977734332106	24.70946302768782	36.16224533303672	18.782313904943347
25	18.746140869493296	25.37421979610957	33.778675211298534	22.1009641230986
26	16.903597672186738	33.16939010170755	26.574679730788958	23.35233249531676
27	18.476102742828733	31.19332514805514	29.017571729323024	21.313000379793106
28	15.478304021766565	27.475202932630218	39.04011689451876	18.006376151084456
29	15.965047626245557	24.16125853643233	38.36628121706816	21.50741262025395
30	19.40709495350505	28.06766116370699	33.21839719704132	19.30684668574664
31	29.631039791765	24.928307515482867	24.249623415409523	21.191029277342608
32	29.895326358259457	26.053997167950786	25.5769979422724	18.47367853151736
33	27.311022033228806	25.748926811159166	25.306722147832204	21.633329007779817
34	19.390553276321587	26.93674282017533	28.49940844490647	25.173295458596613
35	20.25937159689454	24.868652903801117	31.297138432447923	23.57483706685642
36	30.01962660491107	24.045181594817322	26.644649123932812	19.290542676338795
37	20.746590536924778	29.402165723838603	29.407156747126717	20.444086992109906
38	21.16108313761392	32.02116954411043	23.86621775977207	22.951529558503587
39	20.23303800735534	29.812760573007502	26.046201664910303	23.907999754726855
40	22.475861272368697	25.6014301896066	26.783494638452648	25.139213899572056
41	18.36173700919822	22.287343192743336	27.210441030584516	32.14047876747393
42	22.744425858824393	25.91933460628194	24.33285467043323	27.003384864460443
43	22.937934961737867	26.359685460959025	24.968520903118534	25.733858674184575
44	20.46756856834161	29.642210177219354	28.235216945522268	21.655004308916773
45	18.670562516844704	34.661658531298706	22.852612230288667	23.815166721567923
46	22.90537447647731	31.35522443681056	25.88520551370226	19.85419557300988
47	21.913063979689863	27.04174444344624	27.41502545184209	23.630166125021805
48	23.325048234675062	23.59209174736676	29.631467593761123	23.451392424197053
49	20.87226925667502	22.700267186113358	31.924961628537623	24.502501928674
50	19.012376311747868	30.499192642566204	27.667856431551442	22.820574614134483
51	19.47521053799904	32.11186356728874	24.92269855597813	23.490227338734098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1538.0
1	2394.0
2	3250.0
3	9723.0
4	16196.0
5	11516.5
6	6837.0
7	7503.0
8	8169.0
9	8886.0
10	9603.0
11	9733.5
12	9864.0
13	9551.5
14	9239.0
15	8954.0
16	8669.0
17	7893.5
18	7118.0
19	6671.5
20	6225.0
21	5862.5
22	5500.0
23	6416.5
24	7333.0
25	8185.0
26	10733.5
27	12430.0
28	13753.5
29	15077.0
30	18837.0
31	22597.0
32	26381.5
33	30166.0
34	37031.5
35	43897.0
36	46213.0
37	48529.0
38	58581.5
39	68634.0
40	104447.0
41	140260.0
42	172556.5
43	204853.0
44	217577.0
45	230301.0
46	212914.5
47	195528.0
48	184556.5
49	173585.0
50	166235.0
51	158885.0
52	148603.5
53	138322.0
54	125421.5
55	112521.0
56	105041.5
57	97562.0
58	88290.5
59	79019.0
60	73776.0
61	68533.0
62	59942.5
63	51352.0
64	42431.5
65	33511.0
66	28158.5
67	22806.0
68	19831.5
69	16857.0
70	14553.0
71	12249.0
72	10469.0
73	8689.0
74	6395.5
75	3185.0
76	2268.0
77	1897.0
78	1526.0
79	1097.0
80	668.0
81	482.5
82	297.0
83	214.0
84	131.0
85	73.0
86	15.0
87	13.5
88	12.0
89	9.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	3.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2103777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.076138216771188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68652186690693	14.585007214967405
2	7.995522449299367	2.890563378176645
3	2.5630620405794926	1.3899079111102353
4	1.2308201265499166	0.8899389891000038
5	0.7200926359218998	0.6508247007901677
6	0.4989086054782889	0.541100454609727
7	0.3902789888183856	0.4938315861487984
8	0.29203983618121154	0.42231619548918364
9	0.24076720627016396	0.3916927168744807
>10	2.4792203964534476	10.470960496218423
>50	1.2125969578466307	16.350410192375843
>100	1.6763106774174468	47.74615332503147
>500	0.009061138796424055	1.1062114390785713
>1k	0.004264065315964262	1.3375894620674829
>5k	2.6650408224776636E-4	0.24472212300305457
>10k+	2.6650408224776636E-4	0.4887698149584629
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10146	0.4822754502972511	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5080	0.24147046003449987	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	4153	0.1974068544337161	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	2876	0.13670650453921684	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2511	0.11935675691862778	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	2195	0.10433615349915891	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.506711024980309E-5	0.0	0.0	0.040973924517665135	0.0
2	1.4260066537470463E-4	0.0	0.0	0.15191724217918534	0.0
3	1.4260066537470463E-4	0.0	0.0	0.21912968912579614	0.0
4	1.4260066537470463E-4	0.0	0.0	0.3811715785465855	0.0
5	1.4260066537470463E-4	0.0	0.0	0.7670014454954114	0.0
6	1.4260066537470463E-4	0.0	0.0	1.1047748882129618	0.0
7	1.4260066537470463E-4	0.0	0.0	1.377427360409397	0.0
8	1.4260066537470463E-4	0.0	0.0	2.0391419813031515	0.0
9	1.4260066537470463E-4	0.0	0.0	2.335228496176163	0.0
10	1.4260066537470463E-4	0.0	0.0	2.9979888552826655	0.0
11	1.4260066537470463E-4	0.0	0.0	3.3562967938141735	0.0
12	1.4260066537470463E-4	0.0	0.0	3.808340903051987	0.0
13	1.4260066537470463E-4	0.0	0.0	3.951559504643315	0.0
14	1.4260066537470463E-4	0.0	0.0	3.999378261098966	0.0
15	1.4260066537470463E-4	0.0	0.0	4.080042704145924	0.0
16	2.3766777562450774E-4	0.0	0.0	4.226350986820371	0.0
17	2.3766777562450774E-4	0.0	0.0	4.389200946678284	0.0
18	2.3766777562450774E-4	0.0	0.0	4.664135029520715	0.0
19	2.3766777562450774E-4	0.0	0.0	4.802267540713678	0.0
20	2.3766777562450774E-4	0.0	0.0	4.924618911605175	0.0
21	2.3766777562450774E-4	0.0	0.0	5.076346019563861	0.0
22	2.3766777562450774E-4	0.0	0.0	5.233682087027285	0.0
23	2.3766777562450774E-4	0.0	0.0	5.406228892130677	0.0
24	2.3766777562450774E-4	0.0	0.0	5.5347596251884115	0.0
25	2.3766777562450774E-4	0.0	0.0	5.634627624505829	0.0
26	2.3766777562450774E-4	0.0	0.0	5.736111764697494	0.0
27	2.3766777562450774E-4	0.0	0.0	5.846104411256516	0.0
28	2.3766777562450774E-4	0.0	0.0	5.949680027873677	0.0
29	2.3766777562450774E-4	0.0	0.0	6.074740811407293	0.0
30	2.3766777562450774E-4	0.0	0.0	6.253086710235923	0.0
31	2.3766777562450774E-4	0.0	0.0	6.393453298519757	0.0
32	2.3766777562450774E-4	0.0	0.0	6.517040541844501	0.0
33	2.3766777562450774E-4	0.0	0.0	6.643337197811365	0.0
34	2.3766777562450774E-4	0.0	0.0	6.777714558149461	0.0
35	2.3766777562450774E-4	0.0	0.0	6.967658644428568	0.0
36	2.3766777562450774E-4	0.0	0.0	7.09946919278992	0.0
37	2.3766777562450774E-4	0.0	0.0	7.231755076702521	0.0
38	2.3766777562450774E-4	0.0	0.0	7.375686681620723	0.0
39	2.8520133074940926E-4	9.506711024980309E-5	0.0	7.57271326761344	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	35	1.2125747E-7	45.000004	32
TCGATAG	85	0.0	45.000004	1
TACGACC	35	1.2125747E-7	45.000004	31
GATATAC	35	1.2125747E-7	45.000004	11
ACACGAT	20	7.034624E-4	45.0	44
ACGCCGT	25	3.891915E-5	45.0	40
GACCGTT	25	3.891915E-5	45.0	44
TAAGTCG	40	6.8193913E-9	45.0	1
TCGCTAC	20	7.034624E-4	45.0	19
ACGTACT	20	7.034624E-4	45.0	30
CAATACG	25	3.891915E-5	45.0	1
TCGGTAA	20	7.034624E-4	45.0	12
TACACGA	20	7.034624E-4	45.0	34
TAATCGC	20	7.034624E-4	45.0	28
TATGCGA	30	2.1663654E-6	44.999996	19
TCACGAC	445	0.0	43.483147	25
CGACGGT	455	0.0	42.52747	28
ATTAGCG	180	0.0	42.500004	1
TAACGCC	170	0.0	42.352943	12
CGTTTTT	6745	0.0	41.997776	1
>>END_MODULE