FastQCFastQC Report
Thu 26 May 2016
SRR1546820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546820_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2039054
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115310.5655073382068351No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26790.1313844557329036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG302.1663327E-645.0000041
TGTTACG700.045.01
TCGTATA253.891872E-545.018
CGGTCTA3050.044.99999631
GCGATAT2100.042.8571439
CGTTTTT73150.042.6623421
TTACGAG501.0822987E-940.51
ATCGGTA501.0822987E-940.527
ATAGGGC5600.039.7767834
TACGTAG1250.039.61
GGCGATA6650.039.5864688
ACGTTAG1050.038.571431
TACGGGT1350.038.3333324
TCACGAC3700.038.3108125
GTTTACG659.094947E-1238.0769271
CTCACGA3800.037.89473724
ATAGGGT6200.037.7419364
TAGCCGT2400.037.50000444
GTAACGA301.14023176E-437.5000042
GTTGATC10500.037.516
Produced by FastQC (version 0.11.5)