##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546820_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2039054
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.986077367249713	33.0	31.0	34.0	30.0	34.0
2	32.39654614345672	34.0	31.0	34.0	31.0	34.0
3	32.48563108186443	34.0	31.0	34.0	31.0	34.0
4	36.07977571952484	37.0	35.0	37.0	35.0	37.0
5	36.035367871571815	37.0	35.0	37.0	35.0	37.0
6	36.09163317891532	37.0	35.0	37.0	35.0	37.0
7	36.269485751726044	37.0	36.0	37.0	35.0	37.0
8	36.146555216291475	37.0	36.0	37.0	35.0	37.0
9	38.01840706523711	39.0	39.0	39.0	35.0	39.0
10	37.75592161855449	39.0	38.0	39.0	35.0	39.0
11	37.49831049104144	39.0	37.0	39.0	35.0	39.0
12	36.72981588520951	39.0	35.0	39.0	33.0	39.0
13	36.50181996160965	39.0	35.0	39.0	33.0	39.0
14	37.54710272508722	40.0	35.0	41.0	33.0	41.0
15	37.743572264393194	40.0	35.0	41.0	33.0	41.0
16	37.85266157737853	40.0	35.0	41.0	34.0	41.0
17	37.81672530496985	40.0	35.0	41.0	34.0	41.0
18	37.75951446111775	39.0	35.0	41.0	34.0	41.0
19	37.693079241648334	39.0	35.0	41.0	34.0	41.0
20	37.558280947929774	39.0	35.0	41.0	34.0	41.0
21	37.412413795809236	39.0	35.0	41.0	33.0	41.0
22	37.38577252000192	39.0	35.0	41.0	33.0	41.0
23	37.31835253014388	39.0	35.0	41.0	33.0	41.0
24	37.27868217320385	39.0	35.0	41.0	33.0	41.0
25	37.16416240079958	39.0	35.0	41.0	33.0	41.0
26	37.11184206009257	39.0	35.0	41.0	33.0	41.0
27	37.0377238660673	39.0	35.0	41.0	33.0	41.0
28	36.99249603002176	38.0	35.0	41.0	33.0	41.0
29	36.912397611833725	38.0	35.0	41.0	33.0	41.0
30	36.87310537141243	38.0	35.0	41.0	33.0	41.0
31	36.67930422637164	38.0	35.0	41.0	32.0	41.0
32	36.53569596489353	38.0	35.0	41.0	32.0	41.0
33	36.43553432130782	38.0	35.0	41.0	31.0	41.0
34	36.294207019529644	38.0	35.0	41.0	31.0	41.0
35	36.181022179893226	38.0	35.0	41.0	31.0	41.0
36	36.0451650618375	38.0	35.0	41.0	31.0	41.0
37	35.759645404192334	38.0	35.0	41.0	30.0	41.0
38	35.82142797591432	38.0	35.0	41.0	30.0	41.0
39	35.71899616194569	37.0	35.0	40.0	30.0	41.0
40	35.580715861374934	37.0	35.0	40.0	30.0	41.0
41	35.5161560213707	37.0	35.0	40.0	30.0	41.0
42	35.50186409972468	37.0	35.0	40.0	30.0	41.0
43	35.38368184462011	36.0	35.0	40.0	30.0	41.0
44	35.24232462700841	36.0	35.0	40.0	29.0	41.0
45	35.197252745635964	36.0	35.0	40.0	29.0	41.0
46	35.052708265695756	36.0	35.0	40.0	29.0	41.0
47	35.02417591687126	35.0	35.0	40.0	29.0	41.0
48	34.88375001348665	35.0	34.0	40.0	28.0	41.0
49	34.85299653662924	36.0	34.0	40.0	28.0	41.0
50	34.720804843814825	35.0	34.0	40.0	28.0	41.0
51	34.16788324389643	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	6.0
13	9.0
14	25.0
15	50.0
16	111.0
17	279.0
18	622.0
19	1216.0
20	2177.0
21	3224.0
22	5183.0
23	7697.0
24	11366.0
25	16962.0
26	23149.0
27	27984.0
28	29723.0
29	31311.0
30	35276.0
31	43201.0
32	54692.0
33	76156.0
34	157964.0
35	341392.0
36	129468.0
37	176099.0
38	280370.0
39	583083.0
40	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.273714183145714	23.08923647926931	29.2912791912328	14.34577014635218
2	30.976080084195907	25.27382796139779	30.562407861684882	13.187684092721428
3	28.919538177998227	25.019347207087208	32.43209841426465	13.62901620064991
4	26.82003517317344	27.037488953210655	31.25532722527211	14.88714864834379
5	23.56122005596713	31.320455466113206	30.228380415624105	14.889944062295553
6	21.15677171864992	41.25364016843105	26.68252042368667	10.90706768923236
7	86.76827587695078	4.3798741965637005	6.820515788203746	2.0313341382817716
8	87.84696236588144	3.5764133760067165	6.349807312606728	2.2268169455051217
9	84.19767205772874	5.019631652717388	7.868452723664994	2.9142435658888877
10	52.743281933681004	22.392687981779787	13.84588147248675	11.018148612052451
11	44.34826149773375	20.345366037387926	20.330849501778765	14.975522963099555
12	38.333168224088226	20.234236072217804	24.69743322148408	16.735162482209887
13	24.08087279689503	33.24409260372702	25.711334766023853	16.963699833354095
14	18.15508564265586	35.971926197148285	28.953671653619768	16.919316506576088
15	17.38713148352128	22.36493001166227	43.211067485216184	17.036871019600266
16	20.671742876843872	18.48141343976177	40.80465745389774	20.042186229496618
17	21.22562717809337	18.75781612453618	27.475142884886814	32.54141381248363
18	25.3084028181696	21.84419833903369	30.81399511734363	22.033403725453077
19	29.676359723675784	23.572892135274497	24.963684139802083	21.78706400124764
20	31.05905974044827	23.391386397809967	23.799958215917773	21.749595645823995
21	25.823543662894654	25.265196507792336	27.559642853990134	21.35161697532287
22	25.29677978121227	21.843953127283537	27.036851402660254	25.822415688843943
23	22.517304593208422	26.936461712146908	25.331894103834422	25.214339590810248
24	22.765606011415098	23.664699414532425	33.37763492286129	20.19205965119119
25	21.906629250623084	23.652684038774844	31.96050717636708	22.480179534234992
26	19.893882163003042	29.938883423391438	26.382920707347623	23.7843137062579
27	21.086199776955393	28.01357884587657	29.32158736355192	21.578634013616117
28	19.08600753094327	25.5630797418803	35.32083995813745	20.030072769038977
29	19.76519503652184	24.15801641349371	34.09929310356665	21.977495446417798
30	22.385576841025298	25.513056544848737	30.992999694956584	21.10836691916938
31	29.776111863638725	24.43765589337016	24.20161506267122	21.584617180319892
32	30.233824116477543	25.68362583825637	24.23005962568917	19.85249041957692
33	27.566999206494774	25.420219376240162	24.830877455918284	22.181903961346784
34	21.676914883078133	26.878787908510514	28.32666520847413	23.117631999937228
35	22.767518663066305	25.595840031700973	29.241942587101665	22.394698718131053
36	30.56221169228476	23.023715899627963	25.755031499901428	20.659040908185855
37	23.82933458358631	27.645221754794136	27.054457606321364	21.47098605529819
38	24.43078996436583	29.36361665752844	22.69822182247258	23.507371555633153
39	23.118759973987938	27.27946390826334	25.558371676277332	24.043404441471388
40	24.95627874494741	24.595130879319527	25.144258072616026	25.304332303117032
41	21.162117334803295	22.301322083672133	27.022187740001	29.514372841523567
42	24.77315460993186	24.77364503343217	24.251245920902537	26.201954435733434
43	24.062285746233304	25.077413349523848	25.634142107075142	25.22615879716771
44	22.810577846393475	27.938004584478882	27.007818331441936	22.24359923768571
45	21.0415221960772	31.147139801103847	23.783283816907254	24.028054185911703
46	24.82808204196652	27.66763410875828	26.01039501651256	21.493888832762647
47	24.214856497179575	25.70613627692057	27.069023184280557	23.0099840416193
48	25.464357491268007	23.665582176832984	28.20293135934605	22.66712897255296
49	23.392465329510646	22.38910789022753	29.454345005085692	24.764081775176138
50	21.74665310482214	28.294738638603977	26.88124002601206	23.07736823056182
51	21.423905399268484	30.76505085201275	24.517840135670756	23.29320361304801
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1843.0
1	2786.5
2	3730.0
3	11751.5
4	19773.0
5	13795.0
6	7817.0
7	8158.0
8	8499.0
9	8956.0
10	9413.0
11	9349.5
12	9286.0
13	8805.5
14	8325.0
15	7852.5
16	7380.0
17	7032.5
18	6685.0
19	6491.0
20	6297.0
21	6053.0
22	5809.0
23	6607.0
24	7405.0
25	7692.5
26	9078.0
27	10176.0
28	12095.0
29	14014.0
30	16523.0
31	19032.0
32	20917.5
33	22803.0
34	27117.0
35	31431.0
36	37056.5
37	42682.0
38	51548.0
39	60414.0
40	85578.5
41	110743.0
42	135786.5
43	160830.0
44	166704.0
45	172578.0
46	167773.5
47	162969.0
48	156776.0
49	150583.0
50	144755.0
51	138927.0
52	138226.5
53	137526.0
54	127699.0
55	117872.0
56	114243.5
57	110615.0
58	106122.0
59	101629.0
60	94610.5
61	87592.0
62	81106.5
63	74621.0
64	67630.0
65	60639.0
66	53219.5
67	45800.0
68	38663.5
69	31527.0
70	27941.5
71	24356.0
72	20616.0
73	16876.0
74	13498.5
75	7882.5
76	5644.0
77	4408.5
78	3173.0
79	2538.0
80	1903.0
81	1542.0
82	1181.0
83	688.5
84	196.0
85	174.0
86	152.0
87	108.0
88	64.0
89	79.0
90	94.0
91	65.0
92	36.0
93	21.5
94	7.0
95	6.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2039054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.684858887619107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48564654310103	15.843485946797323
2	6.468128189356653	2.5464838134903403
3	2.0422328087836408	1.2060319396971595
4	1.1049515049363408	0.8700325780933694
5	0.745575329862529	0.7338272579216975
6	0.5281976887241416	0.6238498180380775
7	0.42863650071351694	0.59063543214399
8	0.3574983990460759	0.5629844430217399
9	0.303548543273012	0.5377779215884424
>10	4.391566486988507	22.603025062166445
>50	2.435607944767955	34.10278588653056
>100	0.70360470839245	18.303832422847403
>500	0.003034959192086073	0.4350765289990937
>1k	0.0015174795960430365	0.46609296996103844
>5k	0.0	0.0
>10k+	2.5291326600717273E-4	0.574077978703347
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11531	0.5655073382068351	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2679	0.1313844557329036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027905097167608115	0.0
2	0.0	0.0	0.0	0.06983630644406671	0.0
3	0.0	0.0	0.0	0.10656902661724506	0.0
4	0.0	0.0	0.0	0.15644509659871686	0.0
5	0.0	0.0	0.0	0.2595811587137957	0.0
6	0.0	0.0	0.0	0.45987011624017804	0.0
7	0.0	0.0	0.0	0.6068500392829224	0.0
8	0.0	0.0	0.0	0.9665266344098783	0.0
9	0.0	0.0	0.0	1.1662761260859202	0.0
10	0.0	0.0	0.0	1.4567049229691809	0.0
11	0.0	0.0	0.0	1.6882828998153065	0.0
12	0.0	0.0	0.0	1.8909258901431742	0.0
13	0.0	0.0	0.0	1.9772404261976386	0.0
14	0.0022069057513925574	0.0	0.0	2.0137769769706932	0.0
15	0.0022069057513925574	0.0	0.0	2.053991703996069	0.0
16	0.002255948101423503	0.0	0.0	2.139374435399945	0.0
17	0.018881304761914103	0.0	0.0	2.2353012720604752	0.0
18	0.018881304761914103	0.0	0.0	2.3822811951032192	0.0
19	0.018881304761914103	0.0	0.0	2.4555504660494525	0.0
20	0.01893034711194505	0.0	0.0	2.5288197369956853	0.0
21	0.01893034711194505	0.0	0.0	2.6261197594570818	0.0
22	0.01893034711194505	0.0	0.0	2.72871635572182	0.0
23	0.01893034711194505	0.0	0.0	2.837933669240736	0.0
24	0.01893034711194505	0.0	0.0	2.923855866494953	0.0
25	0.01893034711194505	0.0	0.0	2.993348876488803	0.0
26	0.023147989214606382	0.0	0.0	3.0730917376391207	0.0
27	0.023147989214606382	0.0	0.0	3.1584254266929666	0.0
28	0.023147989214606382	0.0	0.0	3.2387567960436554	0.0
29	0.023147989214606382	0.0	0.0	3.3345365056540923	0.0
30	0.023147989214606382	0.0	0.0	3.4519929339782074	0.0
31	0.023147989214606382	0.0	0.0	3.549047744689449	0.0
32	0.023147989214606382	0.0	0.0	3.644876496649917	0.0
33	0.023147989214606382	0.0	0.0	3.748061601115027	0.0
34	0.023147989214606382	0.0	0.0	3.8588973121849643	0.0
35	0.023147989214606382	0.0	0.0	3.9907231490681463	0.0
36	0.023147989214606382	0.0	0.0	4.099695250836907	0.0
37	0.023147989214606382	0.0	0.0	4.213424460558671	0.0
38	0.023147989214606382	0.0	0.0	4.367466482005871	0.0
39	0.023147989214606382	0.0	0.0	4.6492147829336545	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.1663327E-6	45.000004	1
TGTTACG	70	0.0	45.0	1
TCGTATA	25	3.891872E-5	45.0	18
CGGTCTA	305	0.0	44.999996	31
GCGATAT	210	0.0	42.857143	9
CGTTTTT	7315	0.0	42.662342	1
TTACGAG	50	1.0822987E-9	40.5	1
ATCGGTA	50	1.0822987E-9	40.5	27
ATAGGGC	560	0.0	39.776783	4
TACGTAG	125	0.0	39.6	1
GGCGATA	665	0.0	39.586468	8
ACGTTAG	105	0.0	38.57143	1
TACGGGT	135	0.0	38.333332	4
TCACGAC	370	0.0	38.31081	25
GTTTACG	65	9.094947E-12	38.076927	1
CTCACGA	380	0.0	37.894737	24
ATAGGGT	620	0.0	37.741936	4
TAGCCGT	240	0.0	37.500004	44
GTAACGA	30	1.14023176E-4	37.500004	2
GTTGATC	1050	0.0	37.5	16
>>END_MODULE