FastQCFastQC Report
Thu 26 May 2016
SRR1546819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546819_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3187548
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197880.6207906516231285No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37310.11704921776864222No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA207.0351723E-445.019
TATTACG502.1827873E-1145.01
CGTTTTT114200.042.221981
CGTAAGG5350.041.6355132
GTTTACG1250.039.61
CAATCGA356.2517774E-638.57142620
TAGGGTA12550.038.1872525
GACCGAT9450.038.0952349
CGGTCTA4400.036.8181831
TAGCATA10550.036.68246530
AGGGTAC9650.036.6062166
TTACGGG8500.036.529413
GGGCGAT36100.036.274247
ACGGGTA1800.036.255
TCGTAAG2050.036.2195131
AGGGCGA20550.036.1313866
TAGGGAC20350.036.0442285
TCGTTAG1250.036.01
GTCGATC504.8836228E-836.012
GGACGTA250.00210766836.08
Produced by FastQC (version 0.11.5)