FastQCFastQC Report
Thu 26 May 2016
SRR1546818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546818_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2882316
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352711.223703438484885No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39850.13825687398605843No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA253.892275E-545.011
TACGCGC207.03506E-445.045
GTATCGA253.892275E-545.012
CGCAATA406.8212103E-945.015
ATACGAC207.03506E-445.011
CGTTTTT191350.043.7418371
TAATGCG1500.043.51
ACGGTCT5550.041.35135330
ACGGGTA5050.040.99015
TATAGCG2150.040.8139531
CGGTCTA5700.040.65789431
CGACGGT5850.040.38461728
CTCACGA6000.040.12524
AGGGTAC16750.040.029856
CCAACGG850.039.7058832
GGCGATA8850.039.406788
TAGGGTC11700.039.230775
TACGGGT4100.038.9634134
ATAGCGG5900.038.8983042
ACGACGG6100.038.72950727
Produced by FastQC (version 0.11.5)