FastQCFastQC Report
Thu 26 May 2016
SRR1546817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546817_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4535386
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT549901.2124657085416763No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC119750.2640348583339985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT302.166922E-645.00000412
CGTTTTT326950.043.9470861
CGGTCTA4100.041.70731731
CTCACGA4250.040.76470624
GACCGAT16950.040.353989
CGACGGT4250.040.23529428
TCTAGCG800.039.3751
TACGCAT403.4612276E-739.37525
GGGACCG21300.038.978877
ACGGTCT4450.038.93258730
TACCGGT1100.038.86363620
TATCGCT356.252363E-638.5714261
TACGTTG1750.038.5714261
GTTTTTT389000.038.5102842
AGGGCGA32700.038.3944936
TATTCCG659.094947E-1238.0769271
ATTAGCG4050.037.7777751
GCGATCG1550.037.7419369
TCACGAC4550.037.58241725
GGCGATA18300.037.58
Produced by FastQC (version 0.11.5)